Recombinant Anti-MLC1 antibody [EPR10942(2)] - N-terminal (ab186436)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR10942(2)] to MLC1 - N-terminal
- Suitable for: WB
- Reacts with: Human
Related conjugates and formulations
Overview
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Product name
Anti-MLC1 antibody [EPR10942(2)] - N-terminal
See all MLC1 primary antibodies -
Description
Rabbit monoclonal [EPR10942(2)] to MLC1 - N-terminal -
Host species
Rabbit -
Tested applications
Suitable for: WBmore details -
Species reactivity
Reacts with: Human -
Immunogen
Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- Human hypothalamus and hippocampus lysates.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR10942(2) -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab186436 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
1/1000 - 1/10000. Detects a band of approximately 32 kDa (predicted molecular weight: 41 kDa).
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Notes |
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WB
1/1000 - 1/10000. Detects a band of approximately 32 kDa (predicted molecular weight: 41 kDa). |
Target
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Function
Regulates the response of astrocytes to hypo-osmosis by promoting calcium influx. -
Tissue specificity
Expressed in the brain, with highest levels found in the amygdala, nucleus caudatus, thalamus and hippocampus. -
Involvement in disease
Leukoencephalopathy, megalencephalic, with subcortical cysts, 1 -
Cellular localization
Membrane. Cell membrane. Cytoplasm, perinuclear region. Endoplasmic reticulum. - Information by UniProt
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Database links
- Entrez Gene: 23209 Human
- Omim: 605908 Human
- SwissProt: Q15049 Human
- Unigene: 517729 Human
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Alternative names
- KIAA0027 antibody
- LVM antibody
- Megalencephalic leukoencephalopathy with subcortical cysts 1 antibody
see all
Images
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Anti-MLC1 antibody [EPR10942(2)] - N-terminal (ab186436) at 1/10000 dilution + Human hypothalamus lysate at 20 µg
Secondary
Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution
Predicted band size: 41 kDaThe observed MW is lower than the predicted 41kDa, which may be due to the high hydrophobicity of the protein.
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Anti-MLC1 antibody [EPR10942(2)] - N-terminal (ab186436) at 1/1000 dilution + Human hippocampus lysate at 10 µg
Secondary
Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution
Predicted band size: 41 kDaThe observed MW is lower than the predicted 41kDa, which may be due to the high hydrophobicity of the protein.
Datasheets and documents
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SDS download
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Datasheet download
Certificate of Compliance
References (1)
ab186436 has been referenced in 1 publication.
- Simon MJ et al. Transcriptional network analysis of human astrocytic endfoot genes reveals region-specific associations with dementia status and tau pathology. Sci Rep 8:12389 (2018). PubMed: 30120299