Recombinant Anti-MLD antibody [EPR9680] - BSA and Azide free (ab232606)
![Western blot - Anti-MLD antibody [EPR9680] - BSA and Azide free (ab232606)](https://www.abcam.com/ps/products/232/ab232606/Images/ab232606-412789-anti-mld-antibody-epr9680-western-blot-human-knockout-lysate.jpg "Western blot - Anti-MLD antibody [EPR9680] - BSA and Azide free (ab232606)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR9680] to MLD - BSA and Azide free
- Suitable for: WB
- Knockout validated
- Reacts with: Human
Overview
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Product name
Anti-MLD antibody [EPR9680] - BSA and Azide free
See all MLD primary antibodies -
Description
Rabbit monoclonal [EPR9680] to MLD - BSA and Azide free -
Host species
Rabbit -
Tested applications
Suitable for: WBmore details -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic peptide within Human MLD aa 1-100. The exact sequence is proprietary.
Database link: O15121 -
Positive control
- WB: HepG2, U87-MG, 293 and T47D cell lysates.
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General notes
ab232606 is the carrier-free version of ab185237 This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.
Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
Ab232606 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.
Maxpar® is a trademark of Fluidigm Canada Inc.
This product was previously labelled as DEGS1
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
Storage buffer
pH: 7.2
Constituent: PBS -
Carrier free
Yes -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR9680 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Compatible Secondaries
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Conjugation kits
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Isotype control
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KO cell lines
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KO cell lysates
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Positive Controls
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Recombinant Protein
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Related Products
Applications
Our Abpromise guarantee covers the use of ab232606 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | Use at an assay dependent concentration. Predicted molecular weight: 38 kDa. |
Target
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Relevance
DEGS1 is a member of the membrane fatty acid desaturase family which is responsible for inserting double bonds into specific positions in fatty acids. It contains three His containing consensus motifs that are characteristic of a group of membrane fatty acid desaturases. It has sphingolipid-delta-4-desaturase activity and converts D-erythro-sphinganine to D-erythro-sphingosine (E-sphing-4-enine). -
Cellular localization
Endoplasmic reticulum and Mitochondrial -
Database links
- Entrez Gene: 8560 Human
- Omim: 615843 Human
- SwissProt: O15121 Human
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Alternative names
- Degenerative spermatocyte homolog 1 antibody
- Degenerative spermatocyte homolog 1 lipid desaturase antibody
- Degenerative spermatocyte homolog lipid desaturase antibody
see all
Images
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Western blot - Anti-MLD antibody [EPR9680] - BSA and Azide free (ab232606)All lanes : Anti-MLD antibody [EPR9680] (ab185237) at 1/1000 dilution
Lane 1 : Wild-type HEK-293T (Human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate
Lane 2 : DEGS1 knockout HEK-293T (Human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate
Lane 3 : U-87 MG (Human glioblastoma-astrocytoma epithelial cell line) whole cell lysate
Lane 4 : T-47D (Human ductal breast epithelial tumor cell line) whole cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) at 1/10000 dilution
Predicted band size: 38 kDa
Observed band size: 38 kDaThis data was developed using ab185237, the same antibody clone in a different buffer formulation.
Lanes 1-4: Merged signal (red and green). Green - ab185237 observed at 38 kDa. Red - loading control ab8245 observed at 36 kDa.
ab185237 Anti-MLD antibody [EPR9680] was shown to specifically react with MLD in wild-type HEK-293T cells. Loss of signal was observed when knockout cell line ab266481 (knockout cell lysate ab257918) was used. Wild-type and MLD knockout samples were subjected to SDS-PAGE. ab185237 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) were incubated overnight at 4°C at 1 in 1000 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.
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![Western blot - Anti-MLD antibody [EPR9680] - BSA and Azide free (ab232606)](https://www.abcam.com/ps/products/232/ab232606/Images/ab232606-308752-232606888888.jpg)
Western blot - Anti-MLD antibody [EPR9680] - BSA and Azide free (ab232606)All lanes : Anti-MLD antibody [EPR9680] (ab185237) at 1/10000 dilution
Lane 1 : U87-MG (Human glioblastoma-astrocytoma epithelial cell line) cell lysate
Lane 2 : HEK-293 (Human epithelial cell line from embryonic kidney) cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
Predicted band size: 38 kDaThis data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab185237).
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![Anti-MLD antibody [EPR9680] - BSA and Azide free (ab232606)](https://www.abcam.com/ps/products/232/ab232606/Images/ab232606-3-benefits-of-recombinant-antibodies.png)
Anti-MLD antibody [EPR9680] - BSA and Azide free (ab232606)
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
Certificate of Compliance
References (0)
ab232606 has not yet been referenced specifically in any publications.
