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Validated using a knockout cell lineRecombinantRabMAb

Recombinant Anti-MLD antibody [EPR9680] - BSA and Azide free (ab232606)

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  • Certificate of Compliance
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Western blot - Anti-MLD antibody [EPR9680] - BSA and Azide free (ab232606)
  • Western blot - Anti-MLD antibody [EPR9680] - BSA and Azide free (ab232606)
  • Anti-MLD antibody [EPR9680] - BSA and Azide free (ab232606)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR9680] to MLD - BSA and Azide free
  • Suitable for: WB
  • Knockout validated
  • Reacts with: Human

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Recombinant Human MLD protein (ab160100)

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Overview

  • Product name

    Anti-MLD antibody [EPR9680] - BSA and Azide free
    See all MLD primary antibodies
  • Description

    Rabbit monoclonal [EPR9680] to MLD - BSA and Azide free
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WBmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Synthetic peptide within Human MLD aa 1-100. The exact sequence is proprietary.
    Database link: O15121

  • Positive control

    • WB: HepG2, U87-MG, 293 and T47D cell lysates.
  • General notes

    ab232606 is the carrier-free version of ab185237 This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

    Ab232606 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product was previously labelled as DEGS1

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C. Do Not Freeze.
  • Storage buffer

    pH: 7.2
    Constituent: PBS
  • Carrier free

    Yes
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Monoclonal
  • Clone number

    EPR9680
  • Isotype

    IgG
  • Research areas

    • Cardiovascular
    • Lipids / Lipoproteins
    • Fatty Acids
    • Metabolism
    • Cancer
    • Cancer Metabolism
    • Metabolic signaling pathway
    • Metabolism of lipids and lipoproteins
    • Metabolism
    • Pathways and Processes
    • Metabolic signaling pathways
    • Lipid and lipoprotein metabolism
    • Lipid metabolism
    • Metabolism
    • Pathways and Processes
    • Metabolic signaling pathways
    • Lipid and lipoprotein metabolism
    • Fatty acids
    • Metabolism
    • Pathways and Processes
    • Redox metabolism
    • Fatty acid oxidation

Associated products

  • Alternative Versions

    • Anti-MLD antibody [EPR9680] (ab185237)
  • Compatible Secondaries

    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
    • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
  • Conjugation kits

    • HRP Conjugation Kit - Lightning-Link® (ab102890)
  • Isotype control

    • Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730)
  • KO cell lines

    • Human DEGS1 (MLD) knockout HEK293T cell line (ab266481)
  • KO cell lysates

    • Human DEGS1 (MLD) knockout HEK293T cell lysate (ab257918)
  • Positive Controls

    • T47D whole cell lysate (ab14899)
  • Recombinant Protein

    • Recombinant Human MLD protein (ab160100)
  • Related Products

    • Recombinant Human MLD protein (ab160100)

Applications

Our Abpromise guarantee covers the use of ab232606 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use at an assay dependent concentration. Predicted molecular weight: 38 kDa.

Target

  • Relevance

    DEGS1 is a member of the membrane fatty acid desaturase family which is responsible for inserting double bonds into specific positions in fatty acids. It contains three His containing consensus motifs that are characteristic of a group of membrane fatty acid desaturases. It has sphingolipid-delta-4-desaturase activity and converts D-erythro-sphinganine to D-erythro-sphingosine (E-sphing-4-enine).
  • Cellular localization

    Endoplasmic reticulum and Mitochondrial
  • Database links

    • Entrez Gene: 8560 Human
    • Omim: 615843 Human
    • SwissProt: O15121 Human
    • Alternative names

      • Degenerative spermatocyte homolog 1 antibody
      • Degenerative spermatocyte homolog 1 lipid desaturase antibody
      • Degenerative spermatocyte homolog lipid desaturase antibody
      • DEGS 1 antibody
      • DEGS antibody
      • Delta 4 desaturase sphingolipid 1 antibody
      • Des 1 antibody
      • DES1 antibody
      • Dihydroceramide desaturase antibody
      • FADS7 antibody
      • Membrane fatty acid (lipid) desaturase antibody
      • Membrane lipid desaturase antibody
      • MGC5079 antibody
      • MIG15 antibody
      • Migration inducing gene 15 protein antibody
      • MLD antibody
      • Sphingolipid delta 4 desaturase antibody
      • Sphingolipid delta(4) desaturase DES1 antibody
      see all

    Images

    • Western blot - Anti-MLD antibody [EPR9680] - BSA and Azide free (ab232606)
      Western blot - Anti-MLD antibody [EPR9680] - BSA and Azide free (ab232606)
      All lanes : Anti-MLD antibody [EPR9680] (ab185237) at 1/1000 dilution

      Lane 1 : Wild-type HEK-293T (Human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate
      Lane 2 : DEGS1 knockout HEK-293T (Human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate
      Lane 3 : U-87 MG (Human glioblastoma-astrocytoma epithelial cell line) whole cell lysate
      Lane 4 : T-47D (Human ductal breast epithelial tumor cell line) whole cell lysate

      Lysates/proteins at 20 µg per lane.

      Secondary
      All lanes : Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) at 1/10000 dilution

      Predicted band size: 38 kDa
      Observed band size: 38 kDa



      This data was developed using ab185237, the same antibody clone in a different buffer formulation.

      Lanes 1-4: Merged signal (red and green). Green - ab185237 observed at 38 kDa. Red - loading control ab8245 observed at 36 kDa.

       ab185237 Anti-MLD antibody [EPR9680] was shown to specifically react with MLD in wild-type HEK-293T cells. Loss of signal was observed when knockout cell line ab266481 (knockout cell lysate ab257918) was used. Wild-type and MLD knockout samples were subjected to SDS-PAGE. ab185237 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) were incubated overnight at 4°C at 1 in 1000 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.

    • Western blot - Anti-MLD antibody [EPR9680] - BSA and Azide free (ab232606)
      Western blot - Anti-MLD antibody [EPR9680] - BSA and Azide free (ab232606)
      All lanes : Anti-MLD antibody [EPR9680] (ab185237) at 1/10000 dilution

      Lane 1 : U87-MG (Human glioblastoma-astrocytoma epithelial cell line) cell lysate
      Lane 2 : HEK-293 (Human epithelial cell line from embryonic kidney) cell lysate

      Lysates/proteins at 20 µg per lane.

      Secondary
      All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

      Predicted band size: 38 kDa



      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab185237).

    • Anti-MLD antibody [EPR9680] - BSA and Azide free (ab232606)
      Anti-MLD antibody [EPR9680] - BSA and Azide free (ab232606)

    Protocols

    To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

    Click here to view the general protocols

    Datasheets and documents

    • Datasheet

    Certificate of Compliance

    To download a Certificate of Compliance, please enter your Lot number below:

  • References (0)

    Publishing research using ab232606? Please let us know so that we can cite the reference in this datasheet.

    ab232606 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

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