Recombinant
RabMAb

Recombinant Anti-MLD antibody [EPR9681] (ab167169)

Submit a review Q&A (1)References (3)

Overview

  • Product name

    Anti-MLD antibody [EPR9681]
    See all MLD primary antibodies

  • Description

    Rabbit monoclonal [EPR9681] to MLD

  • Host species

    Rabbit

  • Tested applications

    Suitable for: WB, IHC-P, ICC/IFmore details
    Unsuitable for: Flow Cyt or IP

  • Species reactivity

    Reacts with: Human

  • Immunogen

    Synthetic peptide within Human MLD (C terminal). The exact sequence is proprietary.

  • Positive control

    • 293T, T47D and HepG2 whole cell lysate (ab7900); Human breast carcinoma tissue.

  • General notes

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

     

     This product was previously labelled as DEGS1

     

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Applications

Our Abpromise guarantee covers the use of ab167169 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Predicted molecular weight: 38 kDa.
IHC-P 1/50 - 1/100. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
ICC/IF 1/100 - 1/250.
  • Application notes
    Is unsuitable for Flow Cyt or IP.

    • Target

    • Relevance

      DEGS1 is a member of the membrane fatty acid desaturase family which is responsible for inserting double bonds into specific positions in fatty acids. It contains three His containing consensus motifs that are characteristic of a group of membrane fatty acid desaturases. It has sphingolipid-delta-4-desaturase activity and converts D-erythro-sphinganine to D-erythro-sphingosine (E-sphing-4-enine).

    • Cellular localization

      Endoplasmic reticulum and Mitochondrial

    • Database links

      • Alternative names

        • Degenerative spermatocyte homolog 1 antibody
        • Degenerative spermatocyte homolog 1 lipid desaturase antibody
        • Degenerative spermatocyte homolog lipid desaturase antibody
        • DEGS 1 antibody
        • DEGS antibody
        • Delta 4 desaturase sphingolipid 1 antibody
        • Des 1 antibody
        • DES1 antibody
        • Dihydroceramide desaturase antibody
        • FADS7 antibody
        • Membrane fatty acid (lipid) desaturase antibody
        • Membrane lipid desaturase antibody
        • MGC5079 antibody
        • MIG15 antibody
        • Migration inducing gene 15 protein antibody
        • MLD antibody
        • Sphingolipid delta 4 desaturase antibody
        • Sphingolipid delta(4) desaturase DES1 antibody
        see all

      Images

      • Western blot - Anti-MLD antibody [EPR9681] (ab167169)
        Western blot - Anti-MLD antibody [EPR9681] (ab167169)
        All lanes : Anti-MLD antibody [EPR9681] (ab167169) at 1/1000 dilution

        Lane 1 : 293T cell lysates
        Lane 2 : T47D cell lysates
        Lane 3 : HepG2 cell lysates

        Lysates/proteins at 10 µg per lane.

        Secondary
        All lanes : Goat anti-rabbit HRP at 1/2000 dilution

        Predicted band size: 38 kDa

      • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MLD antibody [EPR9681] (ab167169)
        Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MLD antibody [EPR9681] (ab167169)

        Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue labeling MLD with ab167169 at 1/50 dilution.

        Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

      References

      This product has been referenced in:

      • White-Gilbertson S  et al. Genetic and pharmacological inhibition of acid ceramidase prevents asymmetric cell division by neosis. J Lipid Res N/A:N/A (2019). Read more (PubMed: 30988134) »
      • Karsai G  et al. DEGS1-associated aberrant sphingolipid metabolism impairs nervous system function in humans. J Clin Invest 129:1229-1239 (2019). Read more (PubMed: 30620338) »
      See all 3 Publications for this product

      Publishing research using ab167169? Please let us know so that we can cite the reference in this datasheet.

      Customer reviews and Q&As

      Show All Reviews Q&A
      Submit a review Submit a question

      Question

      Do you have any data suggesting that this will work with mouse tissue?

      Read More
      Answer

      Unfortunately, this antibody failed all of our initial in-house validation assays in mouse samples. We would not recommend it for use in mouse samples.

      Read More

      Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
      For licensing inquiries, please contact partnerships@abcam.com