Key features and details
- Rabbit polyclonal to MLF1 Interacting Protein/PBIP1 (phospho T78)
- Suitable for: WB, IP, ELISA, IHC-P
- Reacts with: Human
- Isotype: IgG
Product nameAnti-MLF1 Interacting Protein/PBIP1 (phospho T78) antibody
See all MLF1 Interacting Protein/PBIP1 primary antibodies
DescriptionRabbit polyclonal to MLF1 Interacting Protein/PBIP1 (phospho T78)
Tested applicationsSuitable for: WB, IP, ELISA, IHC-Pmore details
Species reactivityReacts with: Human
Predicted to work with: Mouse, Rat, Cow, Dog, Chimpanzee
Synthetic phosphopeptide conjugated to KLH, corresponding to amino acids surrounding Thr78 of Human MLF1 Interacting Protein/PBIP1.
- Human prostate tissue
Storage instructionsShipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Storage bufferpH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 0.88% Sodium chloride, 0.424% Potassium phosphate
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab115570 in the following tested applications.
|WB||1/500 - 1/2000. Predicted molecular weight: 48 kDa.|
|IP||Use at an assay dependent concentration.|
|ELISA||1/5000 - 1/25000.|
|IHC-P||Use a concentration of 10 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.|
FunctionComponent of the CENPA-NAC (nucleosome-associated) complex, a complex that plays a central role in assembly of kinetochore proteins, mitotic progression and chromosome segregation. The CENPA-NAC complex recruits the CENPA-CAD (nucleosome distal) complex and may be involved in incorporation of newly synthesized CENPA into centromeres. Plays an important role in the correct PLK1 localization to the mitotic kinetochores. A scaffold protein responsible for the initial recruitment and maintenance of the kinetochore PLK1 population until its degradation. Involved in transcriptional repression.
Tissue specificityExpressed at high levels in the testis, fetal liver, thymus, bone marrow and at lower levels in the lymph nodes, placenta, colon and spleen. Present in all cell lines examined, including B-cells, T-cells, epithelial cells and fibroblast cells. Expressed at high levels in glioblastoma cell lines.
modificationsPhosphorylated by PLK1 at Thr-78, creating a self-tethering site that specifically interacts with the polo-box domain of PLK1.
Cellular localizationCytoplasm. Nucleus. Chromosome > centromere > kinetochore. Localizes in the kinetochore domain of centromeres. Colocalizes with PLK1 at the interzone between the inner and the outer kinetochore plates.
- Information by UniProt
- CENP U antibody
- CENP-50 antibody
- CENP-U antibody
ab115570 has not yet been referenced specifically in any publications.