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  1. Link

    mlh1-antibody-epr3894-ab92312.pdf

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Epigenetics and Nuclear Signaling DNA / RNA DNA Damage & Repair Mismatch Repair
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Validated using a knockout cell lineRecombinantRabMAb

Recombinant Anti-MLH1 antibody [EPR3894] (ab92312)

  • Datasheet
  • SDS
Reviews (4)Q&A (4)References (50)

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Western blot - Anti-MLH1 antibody [EPR3894] (ab92312)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MLH1 antibody [EPR3894] (ab92312)
  • Western blot - Anti-MLH1 antibody [EPR3894] (ab92312)
  • Western blot - Anti-MLH1 antibody [EPR3894] (ab92312)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MLH1 antibody [EPR3894] (ab92312)
  • Immunocytochemistry/ Immunofluorescence - Anti-MLH1 antibody [EPR3894] (ab92312)
  • Western blot - Anti-MLH1 antibody [EPR3894] (ab92312)
  • Flow Cytometry (Intracellular) - Anti-MLH1 antibody [EPR3894] (ab92312)
  • Western blot - Anti-MLH1 antibody [EPR3894] (ab92312)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MLH1 antibody [EPR3894] (ab92312)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MLH1 antibody [EPR3894] (ab92312)
  • Immunocytochemistry/ Immunofluorescence - Anti-MLH1 antibody [EPR3894] (ab92312)
  • Immunocytochemistry/ Immunofluorescence - Anti-MLH1 antibody [EPR3894] (ab92312)
  • Anti-MLH1 antibody [EPR3894] (ab92312)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR3894] to MLH1
  • Suitable for: Flow Cyt (Intra), WB, ICC/IF, IHC-P
  • Knockout validated
  • Reacts with: Mouse, Rat, Human

Conjugates logo Related conjugates and formulations

Alexa Fluor® 488 Alexa Fluor® 555 Alexa Fluor® 647 Carrier Free

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Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
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Human MLH1 knockout HeLa cell line (ab267223)
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Human MLH1 knockout A549 cell line (ab276105)

View more associated products

Overview

  • Product name

    Anti-MLH1 antibody [EPR3894]
    See all MLH1 primary antibodies
  • Description

    Rabbit monoclonal [EPR3894] to MLH1
  • Host species

    Rabbit
  • Specificity

    The mouse and rat recommendation is based on the WB results. We do not guarantee IHC-P for mouse and rat.
  • Tested applications

    Suitable for: Flow Cyt (Intra), WB, ICC/IF, IHC-Pmore details
    Unsuitable for: IP
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: 293, HeLa, Jurkat, A431, and SW480 cell lysates; Human testis, rat testis and mouse thymus tissue lysates. IHC-P: Human colonic adenocarcinoma and tonsil tissues. ICC/IF: HeLa and SW480 cells. Flow Cyt (intra): HeLa cells.
  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.20
    Preservative: 0.01% Sodium azide
    Constituents: 40% Glycerol, 59% PBS, 0.05% BSA
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Monoclonal
  • Clone number

    EPR3894
  • Isotype

    IgG
  • Research areas

    • Epigenetics and Nuclear Signaling
    • DNA / RNA
    • DNA Damage & Repair
    • Mismatch Repair
    • Cancer
    • Oncoproteins/suppressors
    • Tumor suppressors
    • Other

Associated products

  • Alternative Versions

    • Alexa Fluor® 488 Anti-MLH1 antibody [EPR3894] (ab199237)
    • Alexa Fluor® 647 Anti-MLH1 antibody [EPR3894] (ab199494)
    • Anti-MLH1 antibody [EPR3894] - BSA and Azide free (ab214441)
    • Alexa Fluor® 555 Anti-MLH1 antibody [EPR3894] (ab215303)
  • Compatible Secondaries

    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
    • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
    • Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773)
    • Goat Anti-Rabbit IgG H&L (DyLight® 488) preadsorbed (ab96899)
    • Goat Anti-Rabbit IgG H&L (HRP) (ab97051)
  • Isotype control

    • Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730)
  • KO cell lines

    • Human MLH1 knockout HeLa cell line (ab267223)
  • KO cell lysates

    • Human MLH1 knockout HeLa cell lysate (ab257172)
  • KO cell pellets

    • Human MLH1 knockout HeLa cell pellet (ab278902)
  • Recombinant Protein

    • Recombinant Human MLH1 protein (ab131924)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab92312 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt (Intra)
1/10 - 1/100.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.  

WB (2)
1/2000. Predicted molecular weight: 84 kDa.

For unpurifid use at 1/10000 - 1/50000

ICC/IF (1)
1/500.
IHC-P (1)
Use at an assay dependent concentration.
Notes
Flow Cyt (Intra)
1/10 - 1/100.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.  

WB
1/2000. Predicted molecular weight: 84 kDa.

For unpurifid use at 1/10000 - 1/50000

ICC/IF
1/500.
IHC-P
Use at an assay dependent concentration.
Application notes
Is unsuitable for IP.

Target

  • Function

    Heterodimerizes with PMS2 to form MutL alpha, a component of the post-replicative DNA mismatch repair system (MMR). DNA repair is initiated by MutS alpha (MSH2-MSH6) or MutS beta (MSH2-MSH6) binding to a dsDNA mismatch, then MutL alpha is recruited to the heteroduplex. Assembly of the MutL-MutS-heteroduplex ternary complex in presence of RFC and PCNA is sufficient to activate endonuclease activity of PMS2. It introduces single-strand breaks near the mismatch and thus generates new entry points for the exonuclease EXO1 to degrade the strand containing the mismatch. DNA methylation would prevent cleavage and therefore assure that only the newly mutated DNA strand is going to be corrected. MutL alpha (MLH1-PMS2) interacts physically with the clamp loader subunits of DNA polymerase III, suggesting that it may play a role to recruit the DNA polymerase III to the site of the MMR. Also implicated in DNA damage signaling, a process which induces cell cycle arrest and can lead to apoptosis in case of major DNA damages. Heterodimerizes with MLH3 to form MutL gamma which plays a role in meiosis.
  • Tissue specificity

    Colon, lymphocytes, breast, lung, spleen, testis, prostate, thyroid, gall bladder and heart.
  • Involvement in disease

    Defects in MLH1 are the cause of hereditary non-polyposis colorectal cancer type 2 (HNPCC2) [MIM:609310]. Mutations in more than one gene locus can be involved alone or in combination in the production of the HNPCC phenotype (also called Lynch syndrome). Most families with clinically recognized HNPCC have mutations in either MLH1 or MSH2 genes. HNPCC is an autosomal, dominantly inherited disease associated with marked increase in cancer susceptibility. It is characterized by a familial predisposition to early onset colorectal carcinoma (CRC) and extra-colonic cancers of the gastrointestinal, urological and female reproductive tracts. HNPCC is reported to be the most common form of inherited colorectal cancer in the Western world, and accounts for 15% of all colon cancers. Cancers in HNPCC originate within benign neoplastic polyps termed adenomas. Clinically, HNPCC is often divided into two subgroups. Type I: hereditary predisposition to colorectal cancer, a young age of onset, and carcinoma observed in the proximal colon. Type II: patients have an increased risk for cancers in certain tissues such as the uterus, ovary, breast, stomach, small intestine, skin, and larynx in addition to the colon. Diagnosis of classical HNPCC is based on the Amsterdam criteria: 3 or more relatives affected by colorectal cancer, one a first degree relative of the other two; 2 or more generation affected; 1 or more colorectal cancers presenting before 50 years of age; exclusion of hereditary polyposis syndromes. The term 'suspected HNPCC' or 'incomplete HNPCC' can be used to describe families who do not or only partially fulfill the Amsterdam criteria, but in whom a genetic basis for colon cancer is strongly suspected.
    Defects in MLH1 are a cause of mismatch repair cancer syndrome (MMRCS) [MIM:276300]; also known as Turcot syndrome or brain tumor-polyposis syndrome 1 (BTPS1). MMRCS is an autosomal dominant disorder characterized by malignant tumors of the brain associated with multiple colorectal adenomas. Skin features include sebaceous cysts, hyperpigmented and cafe au lait spots.
    Defects in MLH1 are a cause of Muir-Torre syndrome (MuToS) [MIM:158320]; also abbreviated MTS. MuToS is a rare autosomal dominant disorder characterized by sebaceous neoplasms and visceral malignancy.
    Note=Defects in MLH1 may contribute to lobular carcinoma in situ (LCIS), a non-invasive neoplastic disease of the breast.
    Defects in MLH1 are a cause of susceptibility to endometrial cancer (ENDMC) [MIM:608089].
    Note=Some epigenetic changes can be transmitted unchanged through the germline (termed 'epigenetic inheritance'). Evidence that this mechanism occurs in humans is provided by the identification of individuals in whom 1 allele of the MLH1 gene is epigenetically silenced throughout the soma (implying a germline event). These individuals are affected by HNPCC but does not have identifiable mutations in MLH1, even though it is silenced, which demonstrates that an epimutation can phenocopy a genetic disease.
  • Sequence similarities

    Belongs to the DNA mismatch repair mutL/hexB family.
  • Cellular localization

    Nucleus.
  • Target information above from: UniProt accession P40692 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 4292 Human
    • Entrez Gene: 17350 Mouse
    • Entrez Gene: 81685 Rat
    • Omim: 120436 Human
    • SwissProt: P40692 Human
    • SwissProt: Q9JK91 Mouse
    • SwissProt: P97679 Rat
    • Unigene: 195364 Human
    • Unigene: 196006 Mouse
    • Unigene: 20391 Rat
    see all
  • Alternative names

    • COCA 2 antibody
    • COCA2 antibody
    • DNA mismatch repair protein Mlh1 antibody
    • FCC 2 antibody
    • FCC2 antibody
    • hMLH 1 antibody
    • hMLH1 antibody
    • HNPCC 2 antibody
    • HNPCC antibody
    • HNPCC2 antibody
    • MGC5172 antibody
    • MLH 1 antibody
    • MLH1 antibody
    • MLH1_HUMAN antibody
    • MutL homolog 1 (E. coli) antibody
    • MutL homolog 1 antibody
    • MutL homolog 1 colon cancer nonpolyposis type 2 antibody
    • MutL homolog 1, colon cancer, nonpolyposis type 2 (E. coli) antibody
    • MutL protein homolog 1 antibody
    • MutL, E. coli, homolog of, 1 antibody
    see all

Images

  • Western blot - Anti-MLH1 antibody [EPR3894] (ab92312)
    Western blot - Anti-MLH1 antibody [EPR3894] (ab92312)
    All lanes : Anti-MLH1 antibody [EPR3894] (ab92312) at 1/2000 dilution

    Lane 1 : Wild-type A549 cell lysate
    Lane 2 : MLH1 CRISPR-Cas9 edited A549 cell lysate
    Lane 3 : Jurkat cell lysate
    Lane 4 : HCT 116 cell lysate

    Lysates/proteins at 20 µg per lane.

    Performed under reducing conditions.

    Predicted band size: 84 kDa
    Observed band size: 85 kDa why is the actual band size different from the predicted?



    False colour image of Western blot: Anti-MLH1 antibody [EPR3894] staining at 1/2000 dilution, shown in green; Mouse anti-GAPDH antibody [6C5] (ab8245) loading control staining at 1/20000 dilution, shown in red. In Western blot, ab92312 was shown to bind specifically to MLH1. A band was observed at 85 kDa in wild-type A549 cell lysates with no signal observed at this size in MLH1 CRISPR-Cas9 edited cell line ab276105 (CRISPR-Cas9 edited cell lysate ab283566). The band observed in the CRISPR-Cas9 edited lysate lane below 85 kDa is likely to represent a truncated form of MLH1. This has not been investigated further and the functional properties of the gene product have not been determined. To generate this image, wild-type and MLH1 CRISPR-Cas9 edited A549 cell lysates were analysed. First, samples were run on an SDS-PAGE gel then transferred onto a nitrocellulose membrane. Membranes were blocked in 3 % milk in TBS-0.1 % Tween® 20 (TBS-T) before incubation with primary antibodies overnight at 4°C. Blots were washed four times in TBS-T, incubated with secondary antibodies for 1 h at room temperature, washed again four times then imaged. Secondary antibodies used were Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed (ab216776) at 1/20000 dilution.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MLH1 antibody [EPR3894] (ab92312)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MLH1 antibody [EPR3894] (ab92312)

    Unpurified ab92312 at 1/100 dilution staining MLH1 in Human colonic adenocarcinoma by Immunohistochemistry, Paraffin-embedded tissue. The use of an HRP/AP polymerized antibody is recommended for a secondary antibody. Heat mediated antigen retrieval was performed via the pressure cooker method before commencing with IHC staining protocol.

  • Western blot - Anti-MLH1 antibody [EPR3894] (ab92312)
    Western blot - Anti-MLH1 antibody [EPR3894] (ab92312)
    All lanes : Anti-MLH1 antibody [EPR3894] (ab92312) at 1/10000 dilution

    Lane 1 : Wild-type HeLa cell lysate
    Lane 2 : MLH1 knockout HeLa cell lysate
    Lane 3 : Jurkat cell lysate
    Lane 4 : HCT116 cell lysate

    Lysates/proteins at 20 µg per lane.

    Performed under reducing conditions.

    Predicted band size: 84 kDa
    Observed band size: 85 kDa why is the actual band size different from the predicted?



    Lanes 1-4: Merged signal (red and green). Green - ab92312 observed at 90 kDa. Red - loading control ab8245 observed at 37 kDa.

     ab92312 Anti-MLH1 antibody [EPR3894] was shown to specifically react with MLH1 in wild-type HeLa cells. Loss of signal was observed when knockout cell line ab267223 (knockout cell lysate ab257172) was used. Wild-type and MLH1 knockout samples were subjected to SDS-PAGE. ab92312 and Anti-GAPDH antibody [6C5] - Loading Control were incubated overnight at 4°C at 1 in 10000 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.

     

     

  • Western blot - Anti-MLH1 antibody [EPR3894] (ab92312)
    Western blot - Anti-MLH1 antibody [EPR3894] (ab92312)
    All lanes : Anti-MLH1 antibody [EPR3894] (ab92312) at 1/1000 dilution

    Lane 1 : Wild-type HAP1 cell lysate
    Lane 2 : MLH1 knockout HAP1 cell lysate
    Lane 3 : HCT116 cell lysate
    Lane 4 : 293T cell lysate

    Lysates/proteins at 20 µg per lane.

    Predicted band size: 84 kDa



    Lanes 1 - 4: Merged signal (red and green). Green - ab92312 observed at 88 kDa. Red - loading control, ab8245, observed at 37 kDa.


    Unpurified ab92312 was shown to recognize MLH1 in wild-type HAP1 cells along with additonal cross reactive bands. No band was observed when MLH1 knockout samples were examined. Wild-type and MLH1 knockout samples were subjected to SDS-PAGE. ab92312 and ab8245 (loading control to GAPDH) were both diluted 1/1000 and incubated overnight at 4°C. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1/10,000 dilution for 1 hour at room temperature before imaging.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MLH1 antibody [EPR3894] (ab92312)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MLH1 antibody [EPR3894] (ab92312)

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Human colon tissue sections labeling MLH1 with Purified ab92312 at 1:250 dilution (2.9 µg/ml). Heat mediated antigen retrieval was performed using citrate (pH 6.0)ImmunoHistoProbe one step HRP Polymer (ready to use)was used as the secondary antibody.Negative control:PBS instead of the primary antibody.Hematoxylinwas used as a counterstain

  • Immunocytochemistry/ Immunofluorescence - Anti-MLH1 antibody [EPR3894] (ab92312)
    Immunocytochemistry/ Immunofluorescence - Anti-MLH1 antibody [EPR3894] (ab92312)
    Immunocytochemistry/ Immunofluorescence analysis of SW480 (Human colorectal adenocarcinoma epithelial cell) cells labeling MLH1 with Purified ab92312 at 1:500 dilution (1.6 µg/ml). Cells were fixed in 4% Paraformaldehyde and permeabilized with 0.1% tritonX-100. Cells were counterstained with Ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor®594) 1:200 (2.5 µg/ml). Goat anti rabbit IgG (Alexa Fluor®488, ab150077) was used as the secondary antibody at 1:1000 (2 µg/ml) dilution. DAPI nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control.
  • Western blot - Anti-MLH1 antibody [EPR3894] (ab92312)
    Western blot - Anti-MLH1 antibody [EPR3894] (ab92312)
    All lanes : Anti-MLH1 antibody [EPR3894] (ab92312) at 0.4 µg/ml (purified)

    Lane 1 : HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysates
    Lane 2 : HCT116 (Human colorectal carcinoma epithelial cell) whole cell lysates, negative control
    Lane 3 : Human testis lysates
    Lane 4 : Rat testis lysates
    Lane 5 : Mouse thymus lysates
    Lane 6 : Rat thymus lysates

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

    Predicted band size: 84 kDa



    Blocking and diluting buffer: 5% NFDM/TBST.
    According to PMID:23653048, HCT116 is MLH1 negative cell line.

  • Flow Cytometry (Intracellular) - Anti-MLH1 antibody [EPR3894] (ab92312)
    Flow Cytometry (Intracellular) - Anti-MLH1 antibody [EPR3894] (ab92312)

    Overlay histogram showing HeLa cells stained with unpurifiedab92312 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab92312, 1/100 dilution) for 30 min at 22°C. The secondary antibody used was DyLight® 488 goat anti-rabbit IgG (H+L) (ab96899) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) (1µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. 

     

     

  • Western blot - Anti-MLH1 antibody [EPR3894] (ab92312)
    Western blot - Anti-MLH1 antibody [EPR3894] (ab92312)
    All lanes : Anti-MLH1 antibody [EPR3894] (ab92312) at 1/10000 dilution (Unpurified)

    Lane 1 : 293 cell lysate
    Lane 2 : HeLa cell lysate
    Lane 3 : A431 cell lysate
    Lane 4 : SW480 cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : goat anti-rabbit HRP at 1/2000 dilution

    Predicted band size: 84 kDa

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MLH1 antibody [EPR3894] (ab92312)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MLH1 antibody [EPR3894] (ab92312)Image from Wang X et al., PLoS One. 2011;6(10):e25913. Epub 2011 Oct 12. Fig 3.; doi:10.1371/journal.pone.0025913; October 12, 2011, PLoS ONE 6(10): e25913.

    Unpurified ab92312 staining MLH1 in Human colorectal (top) and gastric tissue (bottom) by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections). Perform heat mediated antigen retrieval via the pressure cooker method before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MLH1 antibody [EPR3894] (ab92312)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MLH1 antibody [EPR3894] (ab92312)

    Unpurified ab92312 at 1/100 dilution staining MLH1 in Human tonsil by Immunohistochemistry, Paraffin-embedded tissue. The use of an HRP/AP polymerized antibody is recommended for a secondary antibody. Heat mediated antigen retrieval was performed via the pressure cooker method before commencing with IHC staining protocol.

  • Immunocytochemistry/ Immunofluorescence - Anti-MLH1 antibody [EPR3894] (ab92312)
    Immunocytochemistry/ Immunofluorescence - Anti-MLH1 antibody [EPR3894] (ab92312)

    Immunocytochemistry/Immunofluorescence analysis of HeLa (human cervix adenocarcinoma) labelling MLH1 with purified ab92312 at 1/1000. Cells were fixed with 4% PFA and permeabilized with 0.1% Triton X-100. An Alexa Fluor® 488-conjugated goat anti-rabbit IgG (1/1000) was used as the secondary antibody (Ab150077). Nuclei counterstained with DAPI (blue).

    Control: PBS only

  • Immunocytochemistry/ Immunofluorescence - Anti-MLH1 antibody [EPR3894] (ab92312)
    Immunocytochemistry/ Immunofluorescence - Anti-MLH1 antibody [EPR3894] (ab92312)Image courtesy of an Abreview submitted by Dr. Kirk McManus, Univ. of Manitoba/Cancer Care MICB, Canada

    Unpurified ab92312 (1/200) staining MLH1 in HeLa cells (green). Cells were fixed in paraformaldehyde, permeabilised with 0.5% Triton X100/PBS and counterstained with DAPI in order to highlight the nucleus (red). For further experimental details please refer to abreview.

    See Abreview

  • Anti-MLH1 antibody [EPR3894] (ab92312)
    Anti-MLH1 antibody [EPR3894] (ab92312)

Protocols

  • Flow cytometry protocols
  • Immunoprecipitation protocols
  • Immunohistochemistry protocols
  • Immunocytochemistry & immunofluorescence protocols
  • Western blot protocols

Click here to view the general protocols

Datasheets and documents

  • SDS download

  • Datasheet download

    Download

References (50)

Publishing research using ab92312? Please let us know so that we can cite the reference in this datasheet.

ab92312 has been referenced in 50 publications.

  • Takano S  et al. Clinical significance of genetic alterations in endoscopically obtained pancreatic cancer specimens. Cancer Med 10:1264-1274 (2021). PubMed: 33455072
  • Zhou Z  et al. lncRNA SNHG4 modulates colorectal cancer cell cycle and cell proliferation through regulating miR-590-3p/CDK1 axis. Aging (Albany NY) 13:9838-9858 (2021). PubMed: 33744866
  • Xuan Y  et al. Inhibition of chaperone-mediated autophagy reduces tumor growth and metastasis and promotes drug sensitivity in colorectal cancer. Mol Med Rep 23:N/A (2021). PubMed: 33760140
  • Ota R  et al. Integrated genetic and epigenetic analysis of cancer-related genes in non-ampullary duodenal adenomas and intramucosal adenocarcinomas. J Pathol 252:330-342 (2020). PubMed: 32770675
  • Li HD  et al. A PoleP286R mouse model of endometrial cancer recapitulates high mutational burden and immunotherapy response. JCI Insight 5:N/A (2020). PubMed: 32699191
View all Publications for this product

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Abreviews
Abreviews
abreview image
Application
Western blot
Sample
Human Cell lysate - whole cell (HeLa)
Gel Running Conditions
Reduced Denaturing (4-12%)
Loading amount
30 µg
Specification
HeLa
Blocking step
Milk as blocking agent for 45 minute(s) · Concentration: 2% · Temperature: 37°C
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Abcam user community

Verified customer

Submitted Jul 13 2015

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-MLH1 antibody [EPR3894]

Excellent
Abreviews
Abreviews
abreview image
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Blocking step
BSA as blocking agent for 10 minute(s) · Concentration: 1% · Temperature: 25°C
Antigen retrieval step
Heat mediated
Sample
Human Tissue sections (endometrial cancer)
Specification
endometrial cancer
Permeabilization
No
Fixative
Formaldehyde
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Abcam user community

Verified customer

Submitted Jul 23 2013

Immunocytochemistry/ Immunofluorescence abreview for Anti-MLH1 antibody [EPR3894]

Excellent
Abreviews
Abreviews
abreview image
Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (HeLa)
Specification
HeLa
Fixative
Paraformaldehyde
Permeabilization
Yes - 0.5% Triton-X100 in PBS
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DR. Kirk Mcmanus

Verified customer

Submitted Apr 18 2012

Western blot abreview for Anti-MLH1 antibody [EPR3894]

Excellent
Abreviews
Abreviews
abreview image
Application
Western blot
Sample
Human Cell lysate - whole cell (Various tumor cell lines)
Loading amount
30 µg
Specification
Various tumor cell lines
Gel Running Conditions
Reduced Denaturing
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
Read More

Abcam user community

Verified customer

Submitted Nov 03 2010

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