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    mlh3-antibody-ab4834.pdf

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Epigenetics and Nuclear Signaling DNA / RNA DNA Damage & Repair Mismatch Repair
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Anti-MLH3 antibody (ab4834)

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Immunocytochemistry/ Immunofluorescence - Anti-MLH3 antibody (ab4834)

    Key features and details

    • Goat polyclonal to MLH3
    • Suitable for: ICC/IF
    • Reacts with: Human
    • Isotype: IgG

    You may also be interested in

    Protein
    Product image
    Recombinant human TNFAIP3 protein (ab198631)
    Secondary
    Product image
    Donkey Anti-Goat IgG H&L (HRP) (ab97110)

    View more associated products

    Overview

    • Product name

      Anti-MLH3 antibody
      See all MLH3 primary antibodies
    • Description

      Goat polyclonal to MLH3
    • Host species

      Goat
    • Tested Applications & Species

      Application Species
      ICC/IF
      Human
      See all applications and species data
    • Immunogen

      Synthetic peptide:

      RQSLQQSMPPCEPP

      , corresponding to C terminal amino acids 1416-1429 of Human MLH3.
      Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI
    • General notes

      Gene Ontology terms - satellite DNA binding; ATP binding; molecular_function unknown; mismatch repair; biological_process unknown; nucleus; cellular_component unknown

    Properties

    • Form

      Liquid
    • Storage instructions

      Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
    • Storage buffer

      pH: 7.30
      Preservative: 0.02% Sodium azide
      Constituents: Tris buffered saline, 0.5% BSA
    • Concentration information loading...
    • Purity

      Immunogen affinity purified
    • Purification notes

      Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
    • Clonality

      Polyclonal
    • Isotype

      IgG
    • Research areas

      • Epigenetics and Nuclear Signaling
      • DNA / RNA
      • DNA Damage & Repair
      • Mismatch Repair

    Associated products

    • Compatible Secondaries

      • Donkey Anti-Goat IgG H&L (Alexa Fluor® 488) (ab150129)
      • Donkey Anti-Goat IgG H&L (HRP) (ab205723)
    • Isotype control

      • Goat IgG, polyclonal - Isotype Control (ab37373)
    • Recombinant Protein

      • Recombinant human TNFAIP3 protein (ab198631)

    Applications

    The Abpromise guarantee

    Our Abpromise guarantee covers the use of ab4834 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Guaranteed

    Tested applications are guaranteed to work and covered by our Abpromise guarantee.

    Predicted

    Predicted to work for this combination of applications and species but not guaranteed.

    Incompatible

    Does not work for this combination of applications and species.

    Application Species
    ICC/IF
    Human
    Application Abreviews Notes
    ICC/IF
    Use a concentration of 10 µg/ml.
    Notes
    ICC/IF
    Use a concentration of 10 µg/ml.

    Target

    • Function

      Probably involved in the repair of mismatches in DNA.
    • Tissue specificity

      Ubiquitous.
    • Involvement in disease

      Defects in MLH3 are the cause of hereditary non-polyposis colorectal cancer type 7 (HNPCC7) [MIM:614385]. Mutations in more than one gene locus can be involved alone or in combination in the production of the HNPCC phenotype (also called Lynch syndrome). Most families with clinically recognized HNPCC have mutations in either MLH1 or MSH2 genes. HNPCC is an autosomal, dominantly inherited disease associated with marked increase in cancer susceptibility. It is characterized by a familial predisposition to early onset colorectal carcinoma (CRC) and extra-colonic cancers of the gastrointestinal, urological and female reproductive tracts. HNPCC is reported to be the most common form of inherited colorectal cancer in the Western world, and accounts for 15% of all colon cancers. Cancers in HNPCC originate within benign neoplastic polyps termed adenomas. Clinically, HNPCC is often divided into two subgroups. Type I: hereditary predisposition to colorectal cancer, a young age of onset, and carcinoma observed in the proximal colon. Type II: patients have an increased risk for cancers in certain tissues such as the uterus, ovary, breast, stomach, small intestine, skin, and larynx in addition to the colon. Diagnosis of classical HNPCC is based on the Amsterdam criteria: 3 or more relatives affected by colorectal cancer, one a first degree relative of the other two; 2 or more generation affected; 1 or more colorectal cancers presenting before 50 years of age; exclusion of hereditary polyposis syndromes. The term 'suspected HNPCC' or 'incomplete HNPCC' can be used to describe families who do not or only partially fulfill the Amsterdam criteria, but in whom a genetic basis for colon cancer is strongly suspected.
      Defects in MLH3 are a cause of colorectal cancer (CRC) [MIM:114500].
    • Sequence similarities

      Belongs to the DNA mismatch repair mutL/hexB family.
    • Cellular localization

      Nucleus.
    • Target information above from: UniProt accession Q9UHC1 The UniProt Consortium
      The Universal Protein Resource (UniProt) in 2010
      Nucleic Acids Res. 38:D142-D148 (2010) .

      Information by UniProt
    • Database links

      • Entrez Gene: 27030 Human
      • GenBank: NP_055196 Human
      • Omim: 604395 Human
      • SwissProt: Q9UHC1 Human
      • Unigene: 436650 Human
      • Alternative names

        • DNA mismatch repair protein Mlh3 antibody
        • HNPCC 7 antibody
        • HNPCC antibody
        • HNPCC7 antibody
        • MGC138372 antibody
        • Mismatch repair gene MLH 3 antibody
        • Mismatch repair gene MLH3 antibody
        • MLH 3 antibody
        • MLH3 antibody
        • MLH3_HUMAN antibody
        • MutL homolog 3 (E. coli) antibody
        • MutL homolog 3 antibody
        • MutL protein homolog 3 antibody
        • S240II117 antibody
        see all

      Images

      • Immunocytochemistry/ Immunofluorescence - Anti-MLH3 antibody (ab4834)
        Immunocytochemistry/ Immunofluorescence - Anti-MLH3 antibody (ab4834)

        Immunofluorescence analysis of paraformaldehyde fixed HeLa cells, permeabilized with 0.15% Triton. Primary incubation with ab4834 for 1hr (10 μg/ml) followed by Alexa Fluor 488 secondary antibody (2 μg/ml), showing nuclear staining. Actin filaments were stained with phalloidin (red) and the nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10 μg/ml) followed by Alexa Fluor 488 secondary antibody (2 μg/ml).

      Protocols

      To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

      Click here to view the general protocols

      Datasheets and documents

      • Datasheet
    • References (0)

      Publishing research using ab4834? Please let us know so that we can cite the reference in this datasheet.

      ab4834 has not yet been referenced specifically in any publications.

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