Overview

  • Product name

    Anti-MLK3 antibody [3D11]
    See all MLK3 primary antibodies
  • Description

    Mouse monoclonal [3D11] to MLK3
  • Host species

    Mouse
  • Tested applications

    Suitable for: IHC-Fr, WBmore details
    Unsuitable for: IHC-P
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse, Rat
  • Immunogen

    Recombinant fragment (GST-tag) corresponding to Human MLK3 aa 741-847. (NP_002410).
    Sequence:

    PPPGTSRSAP GTPGTPRSPP LGLISRPRPS PLRSRIDPWS FVSAGPRPSP LPSPQPAPRR APWTLFPDSD PFWDSPPANP FQGGPQDCRA QTKDMGAQAP WVPEAGP


    Database link: Q16584

  • Positive control

    • Human liver and Human spleen tissues; MLK3 transfected 293T cell line lysate.

Properties

Applications

Our Abpromise guarantee covers the use of ab218969 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-Fr 1/50.
WB Use at an assay dependent concentration. Predicted molecular weight: 93 kDa.
  • Application notes
    Is unsuitable for IHC-P.
  • Target

    • Function

      Activates the JUN N-terminal pathway. Required for serum-stimulated cell proliferation and for mitogen and cytokine activation of MAPK14 (p38), MAPK3 (ERK) and MAPK8 (JNK1). Plays a role in mitogen-stimulated phosphorylation and activation of BRAF, but does not phosphorylate BRAF directly. Influences microtubule organization during the cell cycle.
    • Tissue specificity

      Expressed in a wide variety of normal and neoplastic tissues including fetal lung, liver, heart and kidney, and adult lung, liver, heart, kidney, placenta, skeletal muscle, pancreas and brain.
    • Sequence similarities

      Belongs to the protein kinase superfamily. STE Ser/Thr protein kinase family. MAP kinase kinase kinase subfamily.
      Contains 1 protein kinase domain.
      Contains 1 SH3 domain.
    • Post-translational
      modifications

      Autophosphorylation on serine and threonine residues within the activation loop plays a role in enzyme activation. Thr-277 is likely to be the main autophosphorylation site. Phosphorylation of Ser-555 and Ser-556 is induced by CDC42.
    • Cellular localization

      Cytoplasm > cytoskeleton > centrosome. Location is cell cycle dependent.
    • Information by UniProt
    • Database links

    • Alternative names

      • 2610017K16Rik antibody
      • EC 2.7.11.25 antibody
      • M3K11_HUMAN antibody
      • Map3k11 antibody
      • MEKK11 antibody
      • MGC17114 antibody
      • Mitogen-activated protein kinase kinase kinase 11 antibody
      • Mixed lineage kinase 3 antibody
      • Mixed lineage protein kinase 3 antibody
      • Mlk-3 antibody
      • Mlk3 antibody
      • Protein tyrosine kinase PTK1 antibody
      • PTK1 antibody
      • RHOE antibody
      • SH3 domain containing proline rich kinase antibody
      • SPRK antibody
      • Src-homology 3 domain-containing proline-rich kinase antibody
      see all

    Images

    • All lanes : Anti-MLK3 antibody [3D11] (ab218969)

      Lane 1 : MLK3 transfected 293T cell line lysate
      Lane 2 : Non-transfected 293T cell line lysate

      Predicted band size: 93 kDa

    • Immunohistochemical analysis of frozen Human spleen tissue labeling MLK3 with ab218969 at 1/50 dilution.

    • Immunohistochemical analysis of frozen Human liver tissue labeling MLK3 with ab218969 at 1/50 dilution.

    References

    ab218969 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

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