Overview

  • Product name

    Anti-MLK3 antibody - C-terminal
    See all MLK3 primary antibodies
  • Description

    Rabbit polyclonal to MLK3 - C-terminal
  • Host species

    Rabbit
  • Specificity

    ab198844 detects endogenous levels of total MLK3 protein.
  • Tested applications

    Suitable for: IHC-P, WBmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse, Rat
  • Immunogen

    Synthetic peptide corresponding to Human MLK3 (C terminal). (Residues near the C terminus.)
    Database link: Q16584

  • Positive control

    • Human liver cancer tissue; LO2 cell lysate.

Properties

Applications

Our Abpromise guarantee covers the use of ab198844 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/50 - 1/200.
WB 1/500 - 1/2000. Predicted molecular weight: 93 kDa.

Target

  • Function

    Activates the JUN N-terminal pathway. Required for serum-stimulated cell proliferation and for mitogen and cytokine activation of MAPK14 (p38), MAPK3 (ERK) and MAPK8 (JNK1). Plays a role in mitogen-stimulated phosphorylation and activation of BRAF, but does not phosphorylate BRAF directly. Influences microtubule organization during the cell cycle.
  • Tissue specificity

    Expressed in a wide variety of normal and neoplastic tissues including fetal lung, liver, heart and kidney, and adult lung, liver, heart, kidney, placenta, skeletal muscle, pancreas and brain.
  • Sequence similarities

    Belongs to the protein kinase superfamily. STE Ser/Thr protein kinase family. MAP kinase kinase kinase subfamily.
    Contains 1 protein kinase domain.
    Contains 1 SH3 domain.
  • Post-translational
    modifications

    Autophosphorylation on serine and threonine residues within the activation loop plays a role in enzyme activation. Thr-277 is likely to be the main autophosphorylation site. Phosphorylation of Ser-555 and Ser-556 is induced by CDC42.
  • Cellular localization

    Cytoplasm > cytoskeleton > centrosome. Location is cell cycle dependent.
  • Information by UniProt
  • Database links

  • Alternative names

    • 2610017K16Rik antibody
    • EC 2.7.11.25 antibody
    • M3K11_HUMAN antibody
    • Map3k11 antibody
    • MEKK11 antibody
    • MGC17114 antibody
    • Mitogen-activated protein kinase kinase kinase 11 antibody
    • Mixed lineage kinase 3 antibody
    • Mixed lineage protein kinase 3 antibody
    • Mlk-3 antibody
    • Mlk3 antibody
    • Protein tyrosine kinase PTK1 antibody
    • PTK1 antibody
    • RHOE antibody
    • SH3 domain containing proline rich kinase antibody
    • SPRK antibody
    • Src-homology 3 domain-containing proline-rich kinase antibody
    see all

Images

  • Anti-MLK3 antibody - C-terminal (ab198844) at 1/400 dilution + LO2 cell lysate at 40 µg

    Developed using the ECL technique.

    Predicted band size: 93 kDa


    Exposure time: 40 seconds


    Gel: 6%SDS-PAGE

  • Immunohistochemical analysis of paraffin-embedded Human liver cancer tissue labeling MLK3 with ab198844 at 1/40 dilution.

References

ab198844 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab198844.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

Sign up