Key features and details
- Rabbit polyclonal to MLK3 - C-terminal
- Suitable for: IHC-P, WB
- Reacts with: Human
- Isotype: IgG
Product nameAnti-MLK3 antibody - C-terminal
See all MLK3 primary antibodies
DescriptionRabbit polyclonal to MLK3 - C-terminal
Specificityab198844 detects endogenous levels of total MLK3 protein.
Tested applicationsSuitable for: IHC-P, WBmore details
Species reactivityReacts with: Human
Predicted to work with: Mouse, Rat
Synthetic peptide corresponding to Human MLK3 (C terminal). (Residues near the C terminus.)
Database link: Q16584
- Human liver cancer tissue; LO2 cell lysate.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.30
Preservative: 0.05% Sodium azide
Constituents: 49% PBS, 50% Glycerol
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab198844 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||1/50 - 1/200.|
|WB||1/500 - 1/2000. Predicted molecular weight: 93 kDa.|
FunctionActivates the JUN N-terminal pathway. Required for serum-stimulated cell proliferation and for mitogen and cytokine activation of MAPK14 (p38), MAPK3 (ERK) and MAPK8 (JNK1). Plays a role in mitogen-stimulated phosphorylation and activation of BRAF, but does not phosphorylate BRAF directly. Influences microtubule organization during the cell cycle.
Tissue specificityExpressed in a wide variety of normal and neoplastic tissues including fetal lung, liver, heart and kidney, and adult lung, liver, heart, kidney, placenta, skeletal muscle, pancreas and brain.
Sequence similaritiesBelongs to the protein kinase superfamily. STE Ser/Thr protein kinase family. MAP kinase kinase kinase subfamily.
Contains 1 protein kinase domain.
Contains 1 SH3 domain.
modificationsAutophosphorylation on serine and threonine residues within the activation loop plays a role in enzyme activation. Thr-277 is likely to be the main autophosphorylation site. Phosphorylation of Ser-555 and Ser-556 is induced by CDC42.
Cellular localizationCytoplasm > cytoskeleton > centrosome. Location is cell cycle dependent.
- Information by UniProt
- 2610017K16Rik antibody
- EC 22.214.171.124 antibody
- M3K11_HUMAN antibody
Anti-MLK3 antibody - C-terminal (ab198844) at 1/400 dilution + LO2 cell lysate at 40 µg
Developed using the ECL technique.
Predicted band size: 93 kDa
Exposure time: 40 seconds
Immunohistochemical analysis of paraffin-embedded Human liver cancer tissue labeling MLK3 with ab198844 at 1/40 dilution.
ab198844 has not yet been referenced specifically in any publications.