• Product name

    Anti-MLK3 antibody [EP1460Y]
    See all MLK3 primary antibodies
  • Description

    Rabbit monoclonal [EP1460Y] to MLK3
  • Host species

  • Tested applications

    Suitable for: Flow Cyt, WB, ICC/IFmore details
    Unsuitable for: IHC-Fr,IHC-P or IP
  • Species reactivity

    Reacts with: Mouse, Human
  • Immunogen

    Synthetic peptide within Human MLK3 aa 800-900 (C terminal). The exact sequence is proprietary.

  • Positive control

    • A431 cell lysate or HeLa cells.
  • General notes

    Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.



Our Abpromise guarantee covers the use of ab51068 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt 1/15.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

WB 1/5000. Detects a band of approximately 100 kDa (predicted molecular weight: 93 kDa).
ICC/IF 1/100 - 1/250.
  • Application notes
    Is unsuitable for IHC-Fr,IHC-P or IP.
  • Target

    • Function

      Activates the JUN N-terminal pathway. Required for serum-stimulated cell proliferation and for mitogen and cytokine activation of MAPK14 (p38), MAPK3 (ERK) and MAPK8 (JNK1). Plays a role in mitogen-stimulated phosphorylation and activation of BRAF, but does not phosphorylate BRAF directly. Influences microtubule organization during the cell cycle.
    • Tissue specificity

      Expressed in a wide variety of normal and neoplastic tissues including fetal lung, liver, heart and kidney, and adult lung, liver, heart, kidney, placenta, skeletal muscle, pancreas and brain.
    • Sequence similarities

      Belongs to the protein kinase superfamily. STE Ser/Thr protein kinase family. MAP kinase kinase kinase subfamily.
      Contains 1 protein kinase domain.
      Contains 1 SH3 domain.
    • Post-translational

      Autophosphorylation on serine and threonine residues within the activation loop plays a role in enzyme activation. Thr-277 is likely to be the main autophosphorylation site. Phosphorylation of Ser-555 and Ser-556 is induced by CDC42.
    • Cellular localization

      Cytoplasm > cytoskeleton > centrosome. Location is cell cycle dependent.
    • Information by UniProt
    • Database links

    • Alternative names

      • 2610017K16Rik antibody
      • EC antibody
      • M3K11_HUMAN antibody
      • Map3k11 antibody
      • MEKK11 antibody
      • MGC17114 antibody
      • Mitogen-activated protein kinase kinase kinase 11 antibody
      • Mixed lineage kinase 3 antibody
      • Mixed lineage protein kinase 3 antibody
      • Mlk-3 antibody
      • Mlk3 antibody
      • Protein tyrosine kinase PTK1 antibody
      • PTK1 antibody
      • RHOE antibody
      • SH3 domain containing proline rich kinase antibody
      • SPRK antibody
      • Src-homology 3 domain-containing proline-rich kinase antibody
      see all


    • Lane 1: Wild-type HAP1 cell lysate (20 µg)
      Lane 2: MLK3 knockout HAP1 cell lysate (20 µg)
      Lane 3: A431 cell lysate (20 µg)
      Lane 4: HeLa cell lysate (20 µg)
      Lanes 1 - 4: Merged signal (red and green). Green - ab51068 observed at 105 kDa. Red - loading control, ab8245, observed at 37 kDa.

      ab51068 was shown to specifically react with MLK3 when MLK3 knockout samples were used. Wild-type and MLK3 knockout samples were subjected to SDS-PAGE. ab51068 and ab8245 (loading control to GAPDH) were diluted 1/5000 and 1/1000 respectively and incubated overnight at 4°C. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1/10000 dilution for 1 hour at room temperature before imaging.

    • Anti-MLK3 antibody [EP1460Y] (ab51068) at 1/5000 dilution + A431 cell lysate at 10 µg

      Goat anti-Rabbit HRP labeled at 1/2000 dilution

      Predicted band size: 93 kDa
      Observed band size: 100 kDa why is the actual band size different from the predicted?


    This product has been referenced in:

    • Zhang X  et al. Emodin protects H9c2 cells from hypoxia-induced injury by up-regulating miR-138 expression. Braz J Med Biol Res 52:e7994 (2019). Read more (PubMed: 30810622) »
    • Tomita K  et al. Mixed Lineage Kinase 3 Mediates the Induction of CXCL10 by a STAT1-Dependent Mechanism During Hepatocyte Lipotoxicity. J Cell Biochem 118:3249-3259 (2017). Read more (PubMed: 28262979) »
    See all 9 Publications for this product

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