Overview

  • Product name

    Anti-MLK3 antibody [EP1460Y] - BSA and Azide free
    See all MLK3 primary antibodies
  • Description

    Rabbit monoclonal [EP1460Y] to MLK3 - BSA and Azide free
  • Host species

    Rabbit
  • Tested applications

    Suitable for: Flow Cyt, ICC/IF, WBmore details
    Unsuitable for: IHC-Fr,IHC-P or IP
  • Species reactivity

    Reacts with: Mouse, Human
  • Immunogen

    Synthetic peptide within Human MLK3 aa 800-900 (C terminal). The exact sequence is proprietary.

  • General notes

    ab239840 is the carrier-free version of ab51068 This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

    Ab239840 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    Rat: We have preliminary internal testing data to indicate this antibody may not react with this species. Please contact us for more information.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

Properties

Applications

Our Abpromise guarantee covers the use of ab239840 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt Use at an assay dependent concentration.

ab199376 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

ICC/IF Use at an assay dependent concentration.
WB Use at an assay dependent concentration. Detects a band of approximately 100 kDa (predicted molecular weight: 93 kDa).
  • Application notes
    Is unsuitable for IHC-Fr,IHC-P or IP.
  • Target

    • Function

      Activates the JUN N-terminal pathway. Required for serum-stimulated cell proliferation and for mitogen and cytokine activation of MAPK14 (p38), MAPK3 (ERK) and MAPK8 (JNK1). Plays a role in mitogen-stimulated phosphorylation and activation of BRAF, but does not phosphorylate BRAF directly. Influences microtubule organization during the cell cycle.
    • Tissue specificity

      Expressed in a wide variety of normal and neoplastic tissues including fetal lung, liver, heart and kidney, and adult lung, liver, heart, kidney, placenta, skeletal muscle, pancreas and brain.
    • Sequence similarities

      Belongs to the protein kinase superfamily. STE Ser/Thr protein kinase family. MAP kinase kinase kinase subfamily.
      Contains 1 protein kinase domain.
      Contains 1 SH3 domain.
    • Post-translational
      modifications

      Autophosphorylation on serine and threonine residues within the activation loop plays a role in enzyme activation. Thr-277 is likely to be the main autophosphorylation site. Phosphorylation of Ser-555 and Ser-556 is induced by CDC42.
    • Cellular localization

      Cytoplasm > cytoskeleton > centrosome. Location is cell cycle dependent.
    • Information by UniProt
    • Database links

    • Alternative names

      • 2610017K16Rik antibody
      • EC 2.7.11.25 antibody
      • M3K11_HUMAN antibody
      • Map3k11 antibody
      • MEKK11 antibody
      • MGC17114 antibody
      • Mitogen-activated protein kinase kinase kinase 11 antibody
      • Mixed lineage kinase 3 antibody
      • Mixed lineage protein kinase 3 antibody
      • Mlk-3 antibody
      • Mlk3 antibody
      • Protein tyrosine kinase PTK1 antibody
      • PTK1 antibody
      • RHOE antibody
      • SH3 domain containing proline rich kinase antibody
      • SPRK antibody
      • Src-homology 3 domain-containing proline-rich kinase antibody
      see all

    Images

    • Immunofluorescent staining of HeLa cells using ab51068 (1:100).

      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab51068).

    References

    ab239840 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

    There are currently no Customer reviews or Questions for ab239840.
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    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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