Overview

  • Product name

    Anti-MLK3 antibody [EP1460Y] (Phycoerythrin)
    See all MLK3 primary antibodies
  • Description

    Rabbit monoclonal [EP1460Y] to MLK3 (Phycoerythrin)
  • Host species

    Rabbit
  • Conjugation

    Phycoerythrin. Ex: 488nm, Em: 575nm
  • Tested applications

    Suitable for: Flow Cytmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse
  • Immunogen

    Synthetic peptide within Human MLK3 aa 800 to the C-terminus (C terminal). The exact sequence is proprietary.
    Database link: Q16584

  • Positive control

    • Flow Cyt: HeLa cells.
  • General notes

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

Properties

Applications

Our Abpromise guarantee covers the use of ab216700 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt 1/500.

The cellular localisation of this product has been verified in ICC/IF.

Target

  • Function

    Activates the JUN N-terminal pathway. Required for serum-stimulated cell proliferation and for mitogen and cytokine activation of MAPK14 (p38), MAPK3 (ERK) and MAPK8 (JNK1). Plays a role in mitogen-stimulated phosphorylation and activation of BRAF, but does not phosphorylate BRAF directly. Influences microtubule organization during the cell cycle.
  • Tissue specificity

    Expressed in a wide variety of normal and neoplastic tissues including fetal lung, liver, heart and kidney, and adult lung, liver, heart, kidney, placenta, skeletal muscle, pancreas and brain.
  • Sequence similarities

    Belongs to the protein kinase superfamily. STE Ser/Thr protein kinase family. MAP kinase kinase kinase subfamily.
    Contains 1 protein kinase domain.
    Contains 1 SH3 domain.
  • Post-translational
    modifications

    Autophosphorylation on serine and threonine residues within the activation loop plays a role in enzyme activation. Thr-277 is likely to be the main autophosphorylation site. Phosphorylation of Ser-555 and Ser-556 is induced by CDC42.
  • Cellular localization

    Cytoplasm > cytoskeleton > centrosome. Location is cell cycle dependent.
  • Information by UniProt
  • Database links

  • Alternative names

    • 2610017K16Rik antibody
    • EC 2.7.11.25 antibody
    • M3K11_HUMAN antibody
    • Map3k11 antibody
    • MEKK11 antibody
    • MGC17114 antibody
    • Mitogen-activated protein kinase kinase kinase 11 antibody
    • Mixed lineage kinase 3 antibody
    • Mixed lineage protein kinase 3 antibody
    • Mlk-3 antibody
    • Mlk3 antibody
    • Protein tyrosine kinase PTK1 antibody
    • PTK1 antibody
    • RHOE antibody
    • SH3 domain containing proline rich kinase antibody
    • SPRK antibody
    • Src-homology 3 domain-containing proline-rich kinase antibody
    see all

Images

  • Overlay histogram showing HeLa cells stained with ab216700 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Triton X-100 for 15 min. The cells were then incubated in 1x PBS / 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (ab216700, 1/500 dilution) for 30 min at 22°C.

    Isotype control antibody (black line) was rabbit IgG (monoclonal) Phycoerythrin (ab209478) used at the same concentration and conditions as the primary antibody. Unlabelled sample (blue line) was also used as a control.

    Acquisition of >5,000 events were collected using a 50 mW Yellow/Green laser (561nm) and 586/15 BP bandpass filter.

    This antibody gave a positive signal in HeLa cells fixed with 4% formaldehyde (10 min)/permeabilized with 0.1% PBS-Triton X-100 for 15 min used under the same conditions.

References

ab216700 has not yet been referenced specifically in any publications.

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