Overview

  • Product name

    Anti-MLK3 (phospho T277 + S281) antibody
    See all MLK3 primary antibodies
  • Description

    Rabbit polyclonal to MLK3 (phospho T277 + S281)
  • Host species

    Rabbit
  • Specificity

    ab191530 detects endogenous levels of Human MLK3 when phosphorylated at Thr277 and Ser281.
  • Tested applications

    Suitable for: WBmore details
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide corresponding to Human MLK3 (phospho T277 + S281). (around the phosphorylation site of Threonine 277 and Serine 281).
    Database link: Q16584

  • Positive control

    • HEK293T, NIH 3T3 and PC12 whole cell lysates.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.2
    Preservative: 0.05% Sodium azide
    Constituent: 99% PBS
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Purification notes

    ab191530 was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab191530 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/1000. Predicted molecular weight: 93 kDa.

Target

  • Function

    Activates the JUN N-terminal pathway. Required for serum-stimulated cell proliferation and for mitogen and cytokine activation of MAPK14 (p38), MAPK3 (ERK) and MAPK8 (JNK1). Plays a role in mitogen-stimulated phosphorylation and activation of BRAF, but does not phosphorylate BRAF directly. Influences microtubule organization during the cell cycle.
  • Tissue specificity

    Expressed in a wide variety of normal and neoplastic tissues including fetal lung, liver, heart and kidney, and adult lung, liver, heart, kidney, placenta, skeletal muscle, pancreas and brain.
  • Sequence similarities

    Belongs to the protein kinase superfamily. STE Ser/Thr protein kinase family. MAP kinase kinase kinase subfamily.
    Contains 1 protein kinase domain.
    Contains 1 SH3 domain.
  • Post-translational
    modifications

    Autophosphorylation on serine and threonine residues within the activation loop plays a role in enzyme activation. Thr-277 is likely to be the main autophosphorylation site. Phosphorylation of Ser-555 and Ser-556 is induced by CDC42.
  • Cellular localization

    Cytoplasm > cytoskeleton > centrosome. Location is cell cycle dependent.
  • Information by UniProt
  • Database links

  • Alternative names

    • 2610017K16Rik antibody
    • EC 2.7.11.25 antibody
    • M3K11_HUMAN antibody
    • Map3k11 antibody
    • MEKK11 antibody
    • MGC17114 antibody
    • Mitogen-activated protein kinase kinase kinase 11 antibody
    • Mixed lineage kinase 3 antibody
    • Mixed lineage protein kinase 3 antibody
    • Mlk-3 antibody
    • Mlk3 antibody
    • Protein tyrosine kinase PTK1 antibody
    • PTK1 antibody
    • RHOE antibody
    • SH3 domain containing proline rich kinase antibody
    • SPRK antibody
    • Src-homology 3 domain-containing proline-rich kinase antibody
    see all

Images

  • All lanes : Anti-MLK3 (phospho T277 + S281) antibody (ab191530)

    Lane 1 : HEK293T whole cell lysate
    Lane 2 : NIH 3T3 whole cell lysate
    Lane 3 : PC12 whole cell lysate

    Predicted band size: 93 kDa

References

This product has been referenced in:

  • Yin M  et al. Protective effects of CXCR3/HO-1 gene-modified BMMSCs on damaged intestinal epithelial cells: Role of the p38-MAPK signaling pathway. Int J Mol Med 43:2086-2102 (2019). Read more (PubMed: 30864680) »
  • Tomita K  et al. Mixed Lineage Kinase 3 Mediates the Induction of CXCL10 by a STAT1-Dependent Mechanism During Hepatocyte Lipotoxicity. J Cell Biochem 118:3249-3259 (2017). Read more (PubMed: 28262979) »
See all 2 Publications for this product

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