Overview

  • Product name
    Anti-MLKL antibody - C-terminal
    See all MLKL primary antibodies
  • Description
    Rabbit polyclonal to MLKL - C-terminal
  • Host species
    Rabbit
  • Tested applications
    Suitable for: WBmore details
  • Species reactivity
    Reacts with: Mouse
  • Immunogen

    Synthetic peptide within Mouse MLKL aa 443-472 (C terminal) conjugated to Keyhole Limpet Haemocyanin (KLH). The exact sequence is proprietary.
    Database link: Q9D2Y4

  • Positive control
    • WB: NIH-3T3 cell lysate and mouse spleen, lung, testis and liver tissue lysates.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer
    Preservative: 0.09% Sodium azide
    Constituent: 100% PBS
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Purification notes
    ab172868 is purified through a protein A column, followed by peptide affinity purification.
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab172868 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000. Predicted molecular weight: 53 kDa.

Target

  • Sequence similarities
    Belongs to the protein kinase superfamily.
    Contains 1 protein kinase domain.
  • Domain
    The protein kinase domain is predicted to be catalytically inactive.
  • Information by UniProt
  • Database links
  • Alternative names
    • 9130019I15Rik antibody
    • FLJ34389 antibody
    • hMLKL antibody
    • Mixed lineage kinase domain like antibody
    • Mixed lineage kinase domain like protein antibody
    • Mixed lineage kinase domain like pseudokinase antibody
    • Mixed lineage kinase domain-like protein antibody
    • Mlkl antibody
    • MLKL_HUMAN antibody
    see all

Images

  • Anti-MLKL antibody - C-terminal (ab172868) at 1/1000 dilution + Mouse spleen tissue lysate at 35 µg

    Secondary
    Peroxidase-conjugated goat anti-rabbit IgG (H+L) at 1/5000 dilution

    Predicted band size: 53 kDa



    Incubation time was overnight at 4°C. Blocking/Dilution buffer: 5% NFDM/TBST.

  • Anti-MLKL antibody - C-terminal (ab172868) at 1/1000 dilution + Mouse lung tissue lysate at 35 µg

    Secondary
    Peroxidase-conjugated goat anti-rabbit IgG (H+L) at 1/5000 dilution

    Predicted band size: 53 kDa



    Incubation time was overnight at 4°C. Blocking/Dilution buffer: 5% NFDM/TBST.

  • Anti-MLKL antibody - C-terminal (ab172868) at 1/1000 dilution + NIH-3T3 cell lysate at 35 µg

    Secondary
    Peroxidase-conjugated goat anti-rabbit IgG (H+L) at 1/5000 dilution

    Predicted band size: 53 kDa



    Incubation time was overnight at 4°C. Blocking/Dilution buffer: 5% NFDM/TBST.

  • All lanes : Anti-MLKL antibody - C-terminal (ab172868) at 1/2000 dilution

    Lane 1 : Mouse testis lysate
    Lane 2 : Mouse liver lysate
    Lane 3 : NIH/3T3 whole cell lysate

    Secondary
    All lanes : Peroxidase-conjugated goat anti-rabbit IgG (H+L) at 1/10000 dilution

    Predicted band size: 53 kDa



    Blocking/Dilution buffer: 5% NFDM/TBST.

References

This product has been referenced in:
  • Berger AK  et al. Viral RNA at Two Stages of Reovirus Infection Is Required for the Induction of Necroptosis. J Virol 91:N/A (2017). Mouse . Read more (PubMed: 28077640) »
  • Xu Q  et al. The cytoplasmic nuclear receptor RAR? controls RIP1 initiated cell death when cIAP activity is inhibited. Nat Commun 8:425 (2017). Read more (PubMed: 28871172) »

See all 4 Publications for this product

Customer reviews and Q&As

Abcam has not validated the combination of species/application used in this Abreview.
Application
Immunocytochemistry
Sample
Mouse Cultured Cells (Cardiomyocytes)
Permeabilization
Yes - Triton x-100, 0.03%
Specification
Cardiomyocytes
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: RT°C
Fixative
Formaldehyde
Username

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Verified customer

Submitted Jan 31 2018

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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