human: HeLa and HT29 WT cell
mouse: L929 and MEF WT cell
This antibody works well in detection of human MLKL and mouse MLKL. But in L929 and MEF cell lysates, there is one nonspecific band slightly higher than mouse MLKL.
Thank you very much for your valuable feedback. We are pleased that the Anti-MLKL antibody ab184718 is working well for you in human and mouse samples.
We are currently unsure about the nature of the unspecific signal slightly higher than MLKL in the mouse cell lines L929 and MEF. Further dilution of the antibody or blocking with milk may reduce the unspecific binding.
We would like to encourage our customer to share their experience with the MLKL antibody in mouse samples.
Submitted Sep 08 2015
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