Western blot abreview for Anti-MLKL antibody [EPR17514]

Excellent
Abreviews
Application
Western blot
Sample
Human Cell lysate - whole cell (ovary)
Gel Running Conditions
Reduced Denaturing (12)
Loading amount
7 µg
Specification
ovary
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 21°C

Other product details

Incubation time
24 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: 5 % NFDM in TBST
Dilution
1/1000

Secondary antibody

Dilution
1/10000
Name
Non-Abcam antibody was used: goat anti rabbit secondary antibody
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase

Detection

Detection method
ECL
Exposure
10 second(s)
Bands
Specific: 54-55 kDa

Konstantin Bagnjuk

Verified customer

Submitted Aug 04 2017

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