Application
Immunohistochemistry (PFA perfusion fixed frozen sections)
Sample
Mouse Tissue sections (brain)
Antigen retrieval step
Heat mediated
Permeabilization
Yes - triton 0.2%
Specification
brain
Blocking step
Serum as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5 · Temperature: 20°C
Fixative
Paraformaldehyde
Other product details
Incubation time
1 hour(s) and 0 minute(s) · Temperature: 20°C · Diluent: DK serum 5%
Dilution
1/10
Secondary antibody
Dilution
1/250
Name
Abcam antibody:Donkey Anti-Rabbit IgG H&L (DyLight® 594) preadsorbed
Additional data
Additional Notes
I try several methods, with or without antigene retrieval (2 methods), permeabilization (2 method) and it's still very weak, even I think it's real.
Abcam response
We would like to thank this researcher for submitting their review. This antibody had not been tested in IHC and we were unsure of what the results would be. The reviewer received an AbTrial discount code for using the antibody in an untested application.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
MRS. Francoise Geffroy
Verified customer
Submitted Mar 05 2018