Recombinant
RabMAb

Recombinant Anti-MLKL (phospho S358) antibody [EPR9514] - BSA and Azide free (ab208909)

Overview

  • Product name

    Anti-MLKL (phospho S358) antibody [EPR9514] - BSA and Azide free
    See all MLKL primary antibodies
  • Description

    Rabbit monoclonal [EPR9514] to MLKL (phospho S358) - BSA and Azide free
  • Host species

    Rabbit
  • Specificity

    Stimulation may be required to allow detection of the phosphorylated protein. Please see images below for recommended treatment conditions and positive controls.
  • Tested applications

    Suitable for: Dot blot, WB, IHC-Pmore details
  • Species reactivity

    Reacts with: Human
    Does not react with: Mouse, Rat
  • Immunogen

    Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) within Human MLKL aa 350-450 (phospho S358). The exact sequence is proprietary.
    Database link: Q8NB16

  • Positive control

    • HT-29 cell lysate treated with TNF alpha+ Smac mimetic+ z-VAD HT-29 cells were treated with the indicated stimuli for 8 hr and then harvested. The ?nal concentrations of 20 ng/ml TNF-a, 100 nM Smac mimetic, and 20 mM z-VAD were used to induce necrosis
  • General notes

    ab208909 is the carrier-free version of ab187091 This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

    Ab208909 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This antibody was developed through collaboration with the lab of Xiaodong Wang at the National Institute of Biological Sciences, Beijing.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab208909 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Dot blot Use at an assay dependent concentration.
WB Use at an assay dependent concentration. Predicted molecular weight: 54 kDa.Can be blocked with Human MLKL (phospho S358) peptide (ab206929).
IHC-P Use at an assay dependent concentration.

Not Suitable for Mouse and Rat

Target

  • Sequence similarities

    Belongs to the protein kinase superfamily.
    Contains 1 protein kinase domain.
  • Domain

    The protein kinase domain is predicted to be catalytically inactive.
  • Information by UniProt
  • Database links

  • Alternative names

    • 9130019I15Rik antibody
    • FLJ34389 antibody
    • hMLKL antibody
    • Mixed lineage kinase domain like antibody
    • Mixed lineage kinase domain like protein antibody
    • Mixed lineage kinase domain like pseudokinase antibody
    • Mixed lineage kinase domain-like protein antibody
    • Mlkl antibody
    • MLKL_HUMAN antibody
    see all

Images

  • Dot blot analysis of MLKL peptides using ab187091 at 1/1000 dilution followed by Goat Anti-Rabbit IgG, (H+L),Peroxidase conjugated secondary antibody at 1/1000 dilution. Blocking and diluting buffer was 5% NFDM/TBST, exposure time 3 minuts.

     

    Lane 1: MLKL (pT357) phospho peptide

    Lane 2: MLKL (pS358) phospho peptide

    Lane 3: MLKL (pT357/pS358) phospho peptide

    Lane 4: MLKL non-phospho peptide 

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab187091).

  • ab187091 at 1:250 staining MLKL (phospho S358) in Human melanoma tissue by immunohistochemistry (FFPE).

     

    Antigen retrival required on FFPE tissue:  HIER using 10mM Citrate buffer pH 6.0, see recommended HIER protocol

     

    For additional IHC guideline, please see IHC resource page

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab187091).

  • This IHC data was generated using the same anti-MLKL (phospho S358) in a different buffer formulation (cat# ab187091).

    ab187091 at 1:250 staining MLKL (phospho S358) in Human skin tissue by immunohistochemistry (FFPE).

     

    Antigen retrival required on FFPE tissue: HIER using 10mM Citrate buffer pH 6.0, see recommended HIER protocol

    For additional IHC guideline, please see IHC resource page

References

This product has been referenced in:

  • Wang H  et al. Mixed Lineage Kinase Domain-like Protein MLKL Causes Necrotic Membrane Disruption upon Phosphorylation by RIP3. Mol Cell N/A:N/A (2014). Read more (PubMed: 24703947) »
  • McCabe KE  et al. Triggering necroptosis in cisplatin and IAP antagonist-resistant ovarian carcinoma. Cell Death Dis 5:e1496 (2014). Read more (PubMed: 25356865) »
See all 4 Publications for this product

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