Overview

  • Product name

    Anti-MLKL (phospho Y222) antibody [EPR19816]
    See all MLKL primary antibodies
  • Description

    Rabbit monoclonal [EPR19816] to MLKL (phospho Y222)
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WBmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Synthetic peptide within Human MLKL aa 200-300 (phospho Y218 + Y222). The exact sequence is proprietary.
    Database link: Q8NB16

  • Positive control

    • WB: HEK-293T transfected with NPM (nucleophosmin)-ALK (anaplastic lymphoma kinase) and MLKL-WT expression vector, whole cell lysate; HEK-293T transfected with NPM (nucleophosmin)-ALK (anaplastic lymphoma kinase) and MLKL-Y23F mutant expression vector, whole cell lysate; HEK-293T transfected with NPM (nucleophosmin)-ALK (anaplastic lymphoma kinase) and MLKL-Y218F mutant expression vector, whole cell lysate
  • General notes

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    Preservative: 0.01% Sodium azide
    Constituents: 59% PBS, 40% Glycerol, 0.05% BSA
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Monoclonal
  • Clone number

    EPR19816
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab206336 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000. Detects a band of approximately 54 kDa (predicted molecular weight: 54 kDa).

Target

  • Sequence similarities

    Belongs to the protein kinase superfamily.
    Contains 1 protein kinase domain.
  • Domain

    The protein kinase domain is predicted to be catalytically inactive.
  • Information by UniProt
  • Database links

  • Alternative names

    • 9130019I15Rik antibody
    • FLJ34389 antibody
    • hMLKL antibody
    • Mixed lineage kinase domain like antibody
    • Mixed lineage kinase domain like protein antibody
    • Mixed lineage kinase domain like pseudokinase antibody
    • Mixed lineage kinase domain-like protein antibody
    • Mlkl antibody
    • MLKL_HUMAN antibody
    see all

Images

  • All lanes : Anti-MLKL (phospho Y222) antibody [EPR19816] (ab206336) at 1/1000 dilution

    Lane 1 : HEK-293T (Human epithelial cell line from embryonic kidney) transfected with MLKL-WT expression vector, whole cell lysate at 20 µg
    Lane 2 : HEK-293T (Human epithelial cell line from embryonic kidney) transfected with NPM (nucleophosmin)-ALK (anaplastic lymphoma kinase) and MLKL-WT expression vector, whole cell lysate at 4 µg
    Lane 3 : HEK-293T (Human epithelial cell line from embryonic kidney) transfected with NPM (nucleophosmin)-ALK (anaplastic lymphoma kinase) and MLKL-Y23F mutant expression vector, whole cell lysate at 20 µg
    Lane 4 : HEK-293T (Human epithelial cell line from embryonic kidney) transfected with NPM (nucleophosmin)-ALK (anaplastic lymphoma kinase) and MLKL-Y218F mutant expression vector, whole cell lysate at 20 µg
    Lane 5 : HEK-293T (Human epithelial cell line from embryonic kidney) transfected with NPM (nucleophosmin)-ALK (anaplastic lymphoma kinase) and MLKL-Y222F mutant expression vector, whole cell lysate at 20 µg
    Lane 6 : HEK-293T (Human epithelial cell line from embryonic kidney) transfected with NPM (nucleophosmin)-ALK (anaplastic lymphoma kinase) and MLKL-Y218/222F mutant expression vector, whole cell lysate at 8 µg

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

    Predicted band size: 54 kDa
    Observed band size: 54 kDa


    Exposure time: 1 minute


    Blocking/Dilution buffer: 5% NFDM/TBST.

    Lysates were kindly provided by our collaborator Dr. Xinhua Feng, Zhejiang University.

    The molecular weight of NPM-ALK is around 80 kDa (lane 2) [PMID: 11090048].

    This antibody can potentially cross react with other kinases.

References

ab206336 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab206336.
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