Recombinant Anti-MLLT11 antibody [CHCR115] (ab109016)
- Datasheet
- References (1)
- Protocols
Overview
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Product name
Anti-MLLT11 antibody [CHCR115]
See all MLLT11 primary antibodies -
Description
Rabbit monoclonal [CHCR115] to MLLT11 -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-P, ICCmore details
Unsuitable for: Flow Cyt or IP -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic peptide within Human MLLT11 aa 50-150 (internal sequence). The exact sequence is proprietary.
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Positive control
- Jurkat, K562 and Molt-4 cell lysates; Human thymus tissue
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General notes
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
Storage buffer
pH: 7.20
Preservative: 0.05% Sodium azide
Constituents: 40% Glycerol, 9.85% Tris glycine, 50% Tissue culture supernatant -
Concentration information loading... -
Purity
Tissue culture supernatant -
Clonality
Monoclonal -
Clone number
CHCR115 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Isotype control
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Recombinant Protein
Applications
Our Abpromise guarantee covers the use of ab109016 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | 1/1000 - 1/10000. Predicted molecular weight: 10 kDa. | |
| IHC-P | 1/100 - 1/250. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. | |
| ICC | 1/250 - 1/500. |
Target
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Tissue specificity
Highly expressed in thymus and all leukemic cell lines. -
Involvement in disease
A chromosomal aberration involving MLLT11 is found in acute leukemias. Translocation t(1;11)(q21;q23) with KMT2A/MLL1. -
Sequence similarities
Belongs to the MLLT11 family. - Information by UniProt
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Database links
- Entrez Gene: 10962 Human
- Omim: 604684 Human
- SwissProt: Q13015 Human
- Unigene: 75823 Human
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Alternative names
- AF1Q antibody
- AF1Q_HUMAN antibody
- ALL1 fused gene from chromosome 1q antibody
see all
Images
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![Western blot - Anti-MLLT11 antibody [CHCR115] (ab109016)](https://www.abcam.com/ps/products/109/ab109016/Images/ab109016-121589-anti-mllt11-antibody-chcr115-western-blot.jpg)
Western blot - Anti-MLLT11 antibody [CHCR115] (ab109016)All lanes : Anti-MLLT11 antibody [CHCR115] (ab109016) at 1/1000 dilution
Lane 1 : Jurkat cell lysate
Lane 2 : K562 cell lysate
Lane 3 : Molt4 cell lysate
Lysates/proteins at 10 µg per lane.
Predicted band size: 10 kDa -
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MLLT11 antibody [CHCR115] (ab109016)](https://www.abcam.com/ps/products/109/ab109016/Images/ab109016-121588-anti-mllt11-antibody-chcr115-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MLLT11 antibody [CHCR115] (ab109016)Immunohistochemical analysis of MLLT11 expression in paraffin-embedded Human thymus tissue using ab109016 at 1/100 dilution.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Protocols
Datasheets and documents
References
This product has been referenced in:
- Jin H et al. MicroRNA-411 Downregulation Enhances Tumor Growth by Upregulating MLLT11 Expression in Human Bladder Cancer. Mol Ther Nucleic Acids 11:312-322 (2018). Read more (PubMed: 29858066) »
