Recombinant Anti-MLLT11 antibody [CHCR115] (ab109016)
![Western blot - Anti-MLLT11 antibody [CHCR115] (ab109016)](https://www.abcam.com/ps/products/109/ab109016/Images/ab109016-121589-anti-mllt11-antibody-chcr115-western-blot.jpg "Western blot - Anti-MLLT11 antibody [CHCR115] (ab109016)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [CHCR115] to MLLT11
- Suitable for: WB, IHC-P
- Reacts with: Human
Overview
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Product name
Anti-MLLT11 antibody [CHCR115]
See all MLLT11 primary antibodies -
Description
Rabbit monoclonal [CHCR115] to MLLT11 -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species IHC-P HumanWB Human
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Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- Jurkat, K562 and Molt-4 cell lysates; Human thymus tissue
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General notes
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
Storage buffer
pH: 7.20
Preservative: 0.05% Sodium azide
Constituents: 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine, 50% Tissue culture supernatant -
Concentration information loading... -
Purity
Tissue culture supernatant -
Clonality
Monoclonal -
Clone number
CHCR115 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab109016 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Tested applications are guaranteed to work and covered by our Abpromise guarantee.
Predicted to work for this combination of applications and species but not guaranteed.
Does not work for this combination of applications and species.
| Application | Species |
|---|---|
| IHC-P |
Human
|
| WB |
Human
|
| Application | Abreviews | Notes |
|---|---|---|
| WB |
1/1000 - 1/10000. Predicted molecular weight: 10 kDa.
|
|
| IHC-P | (2) |
1/100 - 1/250. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
|
| Notes |
|---|
|
WB
1/1000 - 1/10000. Predicted molecular weight: 10 kDa. |
|
IHC-P
1/100 - 1/250. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
Target
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Tissue specificity
Highly expressed in thymus and all leukemic cell lines. -
Involvement in disease
A chromosomal aberration involving MLLT11 is found in acute leukemias. Translocation t(1;11)(q21;q23) with KMT2A/MLL1. -
Sequence similarities
Belongs to the MLLT11 family. - Information by UniProt
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Database links
- Entrez Gene: 10962 Human
- Omim: 604684 Human
- SwissProt: Q13015 Human
- Unigene: 75823 Human
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Alternative names
- AF1Q antibody
- AF1Q_HUMAN antibody
- ALL1 fused gene from chromosome 1q antibody
see all
Images
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Western blot - Anti-MLLT11 antibody [CHCR115] (ab109016)All lanes : Anti-MLLT11 antibody [CHCR115] (ab109016) at 1/1000 dilution
Lane 1 : Jurkat cell lysate
Lane 2 : K562 cell lysate
Lane 3 : Molt4 cell lysate
Lysates/proteins at 10 µg per lane.
Predicted band size: 10 kDa -
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MLLT11 antibody [CHCR115] (ab109016)](https://www.abcam.com/ps/products/109/ab109016/Images/ab109016-121588-anti-mllt11-antibody-chcr115-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MLLT11 antibody [CHCR115] (ab109016)Immunohistochemical analysis of MLLT11 expression in paraffin-embedded Human thymus tissue using ab109016 at 1/100 dilution.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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![Anti-MLLT11 antibody [CHCR115] (ab109016)](https://www.abcam.com/ps/products/109/ab109016/Images/ab109016-3-benefits-of-recombinant-antibodies.png)
Anti-MLLT11 antibody [CHCR115] (ab109016)
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (3)
ab109016 has been referenced in 3 publications.
- Huang S et al. BMP2 Is Related to Hirschsprung's Disease and Required for Enteric Nervous System Development. Front Cell Neurosci 13:523 (2019). PubMed: 31849612
- Gruber ES et al. The Oncogene AF1Q is Associated with WNT and STAT Signaling and Offers a Novel Independent Prognostic Marker in Patients with Resectable Esophageal Cancer. Cells 8:N/A (2019). PubMed: 31671695
- Jin H et al. MicroRNA-411 Downregulation Enhances Tumor Growth by Upregulating MLLT11 Expression in Human Bladder Cancer. Mol Ther Nucleic Acids 11:312-322 (2018). PubMed: 29858066