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RecombinantRabMAb

Recombinant Anti-MLLT11 antibody [CHCR115] (ab109016)

  • Datasheet
  • SDS
Reviews (3) Submit a question References (3)

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Western blot - Anti-MLLT11 antibody [CHCR115] (ab109016)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MLLT11 antibody [CHCR115] (ab109016)
  • Anti-MLLT11 antibody [CHCR115] (ab109016)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [CHCR115] to MLLT11
  • Suitable for: WB, IHC-P
  • Reacts with: Human

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Overview

  • Product name

    Anti-MLLT11 antibody [CHCR115]
    See all MLLT11 primary antibodies
  • Description

    Rabbit monoclonal [CHCR115] to MLLT11
  • Host species

    Rabbit
  • Tested Applications & Species

    Application Species
    IHC-P
    Human
    WB
    Human
    See all applications and species data
  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • Jurkat, K562 and Molt-4 cell lysates; Human thymus tissue
  • General notes

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

     

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
  • Storage buffer

    pH: 7.20
    Preservative: 0.05% Sodium azide
    Constituents: 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine, 50% Tissue culture supernatant
  • Concentration information loading...
  • Purity

    Tissue culture supernatant
  • Clonality

    Monoclonal
  • Clone number

    CHCR115
  • Isotype

    IgG
  • Research areas

    • Cell Biology
    • Other Antibodies
    • Other Antibodies

Associated products

  • Alternative Versions

    • Anti-MLLT11 antibody [CHCR115] - BSA and Azide free (ab247747)
  • Isotype control

    • Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730)
  • Recombinant Protein

    • Recombinant Human MLLT11 protein (denatured) (ab181903)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab109016 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Guaranteed

Tested applications are guaranteed to work and covered by our Abpromise guarantee.

Predicted

Predicted to work for this combination of applications and species but not guaranteed.

Incompatible

Does not work for this combination of applications and species.

Application Species
IHC-P
Human
WB
Human
Application Abreviews Notes
WB
1/1000 - 1/10000. Predicted molecular weight: 10 kDa.
IHC-P (2)
1/100 - 1/250. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Notes
WB
1/1000 - 1/10000. Predicted molecular weight: 10 kDa.
IHC-P
1/100 - 1/250. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
  • Application notes
    Is unsuitable for Flow Cyt or IP.
  • Target

    • Tissue specificity

      Highly expressed in thymus and all leukemic cell lines.
    • Involvement in disease

      A chromosomal aberration involving MLLT11 is found in acute leukemias. Translocation t(1;11)(q21;q23) with KMT2A/MLL1.
    • Sequence similarities

      Belongs to the MLLT11 family.
    • Target information above from: UniProt accession Q13015 The UniProt Consortium
      The Universal Protein Resource (UniProt) in 2010
      Nucleic Acids Res. 38:D142-D148 (2010) .

      Information by UniProt
    • Database links

      • Entrez Gene: 10962 Human
      • Omim: 604684 Human
      • SwissProt: Q13015 Human
      • Unigene: 75823 Human
      • Alternative names

        • AF1Q antibody
        • AF1Q_HUMAN antibody
        • ALL1 fused gene from chromosome 1q antibody
        • MLLT 11 antibody
        • MLLT11 antibody
        • Myeloid/lymphoid or mixed lineage leukemia (trithorax homolog, Drosophila) translocated to 11 antibody
        • Protein AF1q antibody
        • RP11 316M1.10 antibody
        see all

      Images

      • Western blot - Anti-MLLT11 antibody [CHCR115] (ab109016)
        Western blot - Anti-MLLT11 antibody [CHCR115] (ab109016)
        All lanes : Anti-MLLT11 antibody [CHCR115] (ab109016) at 1/1000 dilution

        Lane 1 : Jurkat cell lysate
        Lane 2 : K562 cell lysate
        Lane 3 : Molt4 cell lysate

        Lysates/proteins at 10 µg per lane.

        Predicted band size: 10 kDa

      • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MLLT11 antibody [CHCR115] (ab109016)
        Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MLLT11 antibody [CHCR115] (ab109016)

        Immunohistochemical analysis of MLLT11 expression in paraffin-embedded Human thymus tissue using ab109016 at 1/100 dilution.

        Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

      • Anti-MLLT11 antibody [CHCR115] (ab109016)
        Anti-MLLT11 antibody [CHCR115] (ab109016)

      Protocols

      • Western blot protocols
      • Immunohistochemistry protocols

      Click here to view the general protocols

      Datasheets and documents

      • SDS download

      • Datasheet download

        Download

      References (3)

      Publishing research using ab109016? Please let us know so that we can cite the reference in this datasheet.

      ab109016 has been referenced in 3 publications.

      • Huang S  et al. BMP2 Is Related to Hirschsprung's Disease and Required for Enteric Nervous System Development. Front Cell Neurosci 13:523 (2019). PubMed: 31849612
      • Gruber ES  et al. The Oncogene AF1Q is Associated with WNT and STAT Signaling and Offers a Novel Independent Prognostic Marker in Patients with Resectable Esophageal Cancer. Cells 8:N/A (2019). PubMed: 31671695
      • Jin H  et al. MicroRNA-411 Downregulation Enhances Tumor Growth by Upregulating MLLT11 Expression in Human Bladder Cancer. Mol Ther Nucleic Acids 11:312-322 (2018). PubMed: 29858066

      Customer reviews and Q&As

      Show All Reviews Q&A
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      1-3 of 3 Abreviews or Q&A

      Immunohistochemistry (PFA perfusion fixed frozen sections) abreview for Anti-MLLT11 antibody [CHCR115]

      Excellent
      Abreviews
      Abreviews
      abreview image
      Application
      Immunohistochemistry (PFA perfusion fixed frozen sections)
      Sample
      Pig Tissue sections (Kidney)
      Antigen retrieval step
      Heat mediated - Buffer/Enzyme Used: ab64236 100x Citrate Buffer pH 6.0
      Permeabilization
      Yes - 0.05% Tween 20
      Specification
      Kidney
      Blocking step
      Sea Block as blocking agent for 30 minute(s) · Concentration: 100% · Temperature: 22°C
      Fixative
      Paraformaldehyde
      Read More

      Abcam user community

      Verified customer

      Submitted Jun 29 2020

      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-MLLT11 antibody [CHCR115]

      Excellent
      Abreviews
      Abreviews
      abreview image
      Application
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
      Sample
      Pig Tissue sections (Liver)
      Antigen retrieval step
      Heat mediated - Buffer/Enzyme Used: ab64236 100x Citrate Buffer pH 6.0
      Permeabilization
      Yes - 0.05% Tween 20
      Specification
      Liver
      Blocking step
      Sea Block as blocking agent for 30 minute(s) · Concentration: 100% · Temperature: 22°C
      Fixative
      Formaldehyde
      Read More

      Abcam user community

      Verified customer

      Submitted Feb 11 2020

      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-MLLT11 antibody [CHCR115]

      Excellent
      Abreviews
      Abreviews
      abreview image
      Application
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
      Sample
      Human Tissue sections (Tonsil)
      Antigen retrieval step
      Heat mediated - Buffer/Enzyme Used: ab64236 100x Citrate Buffer pH 6.0
      Permeabilization
      Yes - 0.05% Tween 20
      Specification
      Tonsil
      Blocking step
      sea block as blocking agent for 30 minute(s) · Concentration: 100% · Temperature: 22°C
      Fixative
      Formaldehyde
      Read More

      Mr. David Ivancic

      Verified customer

      Submitted Jul 17 2019

      Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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