Anti-MLLT11 antibody [CHCR115] (ab109016)
- Datasheet
- Specific References (1)
- Protocols
Overview
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Product nameAnti-MLLT11 antibody [CHCR115]
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DescriptionRabbit monoclonal [CHCR115] to MLLT11
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Host speciesRabbit
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Tested applicationsSuitable for: WB, IHC-P, ICCmore details
Unsuitable for: Flow Cyt or IP -
Species reactivityReacts with: Human
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Immunogen
Synthetic peptide within Human MLLT11 aa 50-150 (internal sequence). The exact sequence is proprietary.
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Positive control
- Jurkat, K562 and Molt-4 cell lysates; Human thymus tissue
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General notes
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents
This product is a recombinant rabbit monoclonal antibody.
Properties
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FormLiquid
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Storage instructionsShipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
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Storage bufferpH: 7.20
Preservative: 0.05% Sodium azide
Constituents: 40% Glycerol, 9.85% Tris glycine, 50% Tissue culture supernatant -
PurityTissue culture supernatant
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ClonalityMonoclonal
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Clone numberCHCR115
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IsotypeIgG
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Research areas
Applications
Our Abpromise guarantee covers the use of ab109016 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
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| WB | 1/1000 - 1/10000. Predicted molecular weight: 10 kDa. | |
| IHC-P | 1/100 - 1/250. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. | |
| ICC | 1/250 - 1/500. |
Target
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Tissue specificityHighly expressed in thymus and all leukemic cell lines.
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Involvement in diseaseA chromosomal aberration involving MLLT11 is found in acute leukemias. Translocation t(1;11)(q21;q23) with KMT2A/MLL1.
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Sequence similaritiesBelongs to the MLLT11 family.
- Information by UniProt
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Database links
- Entrez Gene: 10962 Human
- Omim: 604684 Human
- SwissProt: Q13015 Human
- Unigene: 75823 Human
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Alternative names
- AF1Q antibody
- AF1Q_HUMAN antibody
- ALL1 fused gene from chromosome 1q antibody
see all
Images
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![Western blot - Anti-MLLT11 antibody [CHCR115] (ab109016)](https://www.abcam.com/ps/products/109/ab109016/Images/MLLT11-Primary-antibodies-ab109016-4.jpg)
Western blot - Anti-MLLT11 antibody [CHCR115] (ab109016)All lanes : Anti-MLLT11 antibody [CHCR115] (ab109016) at 1/1000 dilution
Lane 1 : Jurkat cell lysate
Lane 2 : K562 cell lysate
Lane 3 : Molt4 cell lysate
Lysates/proteins at 10 µg per lane.
Predicted band size: 10 kDa -
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MLLT11 antibody [CHCR115] (ab109016)](https://www.abcam.com/ps/products/109/ab109016/Images/MLLT11-Primary-antibodies-ab109016-3.JPG)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MLLT11 antibody [CHCR115] (ab109016)Immunohistochemical analysis of MLLT11 expression in paraffin-embedded Human thymus tissue using ab109016 at 1/100 dilution.
Protocols
References
This product has been referenced in:
- Jin H et al. MicroRNA-411 Downregulation Enhances Tumor Growth by Upregulating MLLT11 Expression in Human Bladder Cancer. Mol Ther Nucleic Acids 11:312-322 (2018). Read more (PubMed: 29858066) »
Customer reviews and Q&As
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"