• Product name

    Anti-MLN64 antibody
  • Description

    Rabbit polyclonal to MLN64
  • Host species

  • Tested applications

    Suitable for: ICC/IF, IHC-P, ICC, IP, Inhibition Assay, WBmore details
  • Species reactivity

    Reacts with: Mouse, Rat, Hamster, Cow, Human
  • Immunogen

    Other Immunogen Type corresponding to MLN64. Recombinant MLN64 protein.

  • Positive control

    • Rat adrenal tissue lysate - total protein (ab4030) can be used as a positive control in WB.



Our Abpromise guarantee covers the use of ab3478 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use a concentration of 5 µg/ml.
IHC-P 1/50. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
ICC Use at an assay dependent concentration.
IP Use at an assay dependent concentration.
Inhibition Assay Use at an assay dependent concentration.
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 49 kDa. By Western blot, this antibody detects an ~53 kDa protein representing MLN 64 from rat adrenal gland tissue extracts. This antibody also detects an ~25 kDa protein from rat adrenal gland which may correspond to degradation product.


  • Function

    Binds and transports cholesterol. Promotes steroidogenesis in placenta and brain.
  • Sequence similarities

    Contains 1 MENTAL domain.
    Contains 1 START domain.
  • Cellular localization

    Late endosome membrane.
  • Information by UniProt
  • Database links

  • Alternative names

    • CAB 1 antibody
    • CAB1 antibody
    • Es 64 antibody
    • Es64 antibody
    • FLJ41370 antibody
    • Metastatic lymph node gene 64 protein antibody
    • Metastatic lymph node protein 64 antibody
    • MLN 64 antibody
    • Mln64 antibody
    • Protein CAB 1 antibody
    • Protein CAB1 antibody
    • Protein MLN 64 antibody
    • Protein MLN64 antibody
    • StAR related lipid transfer protein 3 antibody
    • StAR-related lipid transfer protein 3 antibody
    • STAR3_HUMAN antibody
    • STARD 3 antibody
    • StARD3 antibody
    • START domain containing 3 antibody
    • START domain-containing protein 3 antibody
    • Steroidogenic acute regulatory protein related antibody
    see all


  • Anti-MLN64 antibody (ab3478) at 1 µg/ml + Lysates prepared from rat adrenal gland

    Predicted band size: 49 kDa

    Western blot of MLN64 on rat adrenal gland extract using ab3478.
  • ab3478 staining MLN64 in Human ovarian cancer tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with 5% BSA for 12 hours 10 minutes at 4°C; antigen retrieval was by heat mediation. Samples were incubated with primary antibody (1/50) for 20 minutes at 25°C. An undiluted HRP-conjugated monoclonal was used as the secondary antibody.

    See Abreview

  • ICC/IF image of ab3478 stained HeLa cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab3478, 5µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899, DyLight® 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.


This product has been referenced in:

  • Lei C  et al. MLN64 deletion suppresses RANKL-induced osteoclastic differentiation and attenuates diabetic osteoporosis in streptozotocin (STZ)-induced mice. Biochem Biophys Res Commun 505:1228-1235 (2018). Read more (PubMed: 30322615) »
  • Balboa E  et al. MLN64 induces mitochondrial dysfunction associated with increased mitochondrial cholesterol content. Redox Biol 12:274-284 (2017). WB . Read more (PubMed: 28282615) »
See all 11 Publications for this product

Customer reviews and Q&As

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Human Tissue sections (Ovarian cancer)
Ovarian cancer
Antigen retrieval step
Heat mediated
Blocking step
BSA as blocking agent for 12 hour(s) and 10 minute(s) · Concentration: 5% · Temperature: 4°C

Mrs. Wu Xin

Verified customer

Submitted Oct 05 2010

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