Key features and details
- Rabbit polyclonal to MLX
- Suitable for: ICC/IF, ELISA, WB
- Reacts with: Human
- Isotype: IgG
Product nameAnti-MLX antibody
See all MLX primary antibodies
DescriptionRabbit polyclonal to MLX
Specificityab65147 detects endogenous levels of total MLX protein.
Tested applicationsSuitable for: ICC/IF, ELISA, WBmore details
Species reactivityReacts with: Human
Predicted to work with: Mouse, Rat
Synthetic peptide derived from an internal sequence of human MLX
- LOVO cell extracts.
Storage instructionsShipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
Storage bufferpH: 7.40
Preservative: 0.02% Sodium azide
Constituents: PBS, 50% Glycerol, 0.87% Sodium chloride
Concentration information loading...
PurityImmunogen affinity purified
- Pathways and Processes
- Metabolic signaling pathways
- Energy transfer pathways
- Integration of energy
Our Abpromise guarantee covers the use of ab65147 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
ICC/IF: Use at a concentration of 1 µg/ml.
WB: 1/500 - 1/1000. Detects a band of approximately 33 kDa (predicted molecular weight: 33 kDa).
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
FunctionTranscription regulator. Forms a sequence-specific DNA-binding protein complex with MAD1, MAD4, MNT, WBSCR14 and MLXIP which recognizes the core sequence 5'-CACGTG-3'. The TCFL4-MAD1, TCFL4-MAD4, TCFL4-WBSCR14 complexes are transcriptional repressors. Plays a role in transcriptional activation of glycolytic target genes. Involved in glucose-responsive gene regulation.
Tissue specificityExpressed in all tissues tested, including spleen, thymus, prostate, ovary, intestine, colon, peripheral blood leukocyte, heart, liver, skeletal muscle and kidney. Lower levels of expression in testis, brain, placenta and lung.
Sequence similaritiesContains 1 basic helix-loop-helix (bHLH) domain.
Cellular localizationCytoplasm. Found predominantly in the cytoplasm and Nucleus. Found predominantly in the nucleus.
- Information by UniProt
- bHLHd13 antibody
- BigMax protein antibody
- Class D basic helix-loop-helix protein 13 antibody
All lanes : Anti-MLX antibody (ab65147) at 1/500 dilution
Lane 1 : LOVO cell extract
Lane 2 : LOVO cell extract with immunising peptide at 5 µg
Lysates/proteins at 5 µg per lane.
Predicted band size: 33 kDa
Observed band size: 33 kDa
ICC/IF image of ab65147 stained HepG2 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab65147, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab65147 has not yet been referenced specifically in any publications.