Overview

  • Product name

  • Description

    Rabbit polyclonal to MLYCD/MCD
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IHC-P, ICC/IFmore details
  • Species reactivity

    Reacts with: Mouse, Human
    Predicted to work with: Rat, Cow
  • Immunogen

    Recombinant fragment corresponding to Human MLYCD/MCD aa 90-274.

  • Positive control

    • 293T; A431; H1299; HepG2 or Raji whole cell lysates HeLa cells (IF)
  • General notes

    Protein previously labeled as MLYCD.

Properties

Applications

Our Abpromise guarantee covers the use of ab95945 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/3000. Predicted molecular weight: 55 kDa.
IHC-P 1/100 - 1/500.
ICC/IF 1/100 - 1/200.

Target

  • Function

    Catalyzes the conversion of malonyl-CoA to acetyl-CoA. In the fatty acid biosynthesis MCD selectively removes malonyl-CoA and thus assures that methyl-malonyl-CoA is the only chain elongating substrate for fatty acid synthase and that fatty acids with multiple methyl side chains are produced. In peroxisomes it may be involved in degrading intraperoxisomal malonyl-CoA, which is generated by the peroxisomal beta-oxidation of odd chain-length dicarboxylic fatty acids.
  • Pathway

    Metabolic intermediate biosynthesis; acetyl-CoA biosynthesis; acetyl-CoA from malonyl-CoA: step 1/1.
  • Involvement in disease

    Malonyl-CoA decarboxylase deficiency (MLYCD deficiency) [MIM:248360]: Autosomal recessive disease characterized by abdominal pain, chronic constipation, episodic vomiting, metabolic acidosis and malonic aciduria. Note=The disease is caused by mutations affecting the gene represented in this entry.
  • Cellular localization

    Mitochondrion. Cytoplasm. Peroxisome.
  • Information by UniProt
  • Database links

  • Alternative names

    • DCMC_HUMAN antibody
    • hMCD antibody
    • Malonyl CoA decarboxylase antibody
    • Malonyl CoA decarboxylase mitochondrial antibody
    • Malonyl coenzyme A decarboxylase antibody
    • Malonyl-CoA decarboxylase antibody
    • MCD antibody
    • MGC59795 antibody
    • mitochondrial antibody
    • Mlycd antibody
    see all

Images

  • Anti-MLYCD/MCD antibody (ab95945) at 1/1000 dilution + HepG2 whole cell lysate at 30 µg

    Predicted band size: 55 kDa



    Gel concentration: 7.5%
  • Immunofluorescence analysis of paraformaldehyde fixed HeLa cells using: (1) ab95945 at 1/200 dilution, (2) merged with DNA probe.
  • Immunohistochemical analysis of paraffin embedded H661 (human) xenograft, using ab95945 at 1/500 dilution.

References

This product has been referenced in:

  • Karwi QG  et al. Targeting the glucagon receptor improves cardiac function and enhances insulin sensitivity following a myocardial infarction. Cardiovasc Diabetol 18:1 (2019). Read more (PubMed: 30626440) »
  • Mazibuko-Mbeje SE  et al. Aspalathin-Enriched Green Rooibos Extract Reduces Hepatic Insulin Resistance by Modulating PI3K/AKT and AMPK Pathways. Int J Mol Sci 20:N/A (2019). Read more (PubMed: 30717198) »
See all 5 Publications for this product

Customer reviews and Q&As

1-3 of 3 Abreviews or Q&A

Application
Western blot
Sample
Rat Cell lysate - whole cell (whole rat heart lysate and rat insulinoma cells)
Gel Running Conditions
Reduced Denaturing (4-20% gradient)
Loading amount
50 µg
Specification
whole rat heart lysate and rat insulinoma cells
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 4°C

Abcam user community

Verified customer

Submitted Oct 13 2015

Question
Answer

I am very pleased to hear you would like to accept our offer and test ab95945 in pig. This code will give you: 1 free primary antibody before the expiration date. To redeem this offer, please submit an Abreview for pig and include this code in the “Additional Comments” section so we know the Abreview is for this promotion. For more information on how to submit an Abreview, please visit the site: www.abcam.com/Abreviews.
Remember, we publish both positive and negative Abreviews on our datasheets so please submit the results of your tests. The code will be active once the Abreview has been submitted and can be redeemed in one of the following ways: 1) Call to place your order and mention the code to our customer service department; 2) Include the code in your fax order; 3) Place your order on the web and enter the promotional code.

Any feedback that you can provide will be greatly appreciated, whether positive or negative. If you have any further questions, please do not hesitate to contact us. We look forward to receiving your Abreview and wish you luck with your research.

The terms and conditions applicable to this offer can be found here: www.abcam.com/collaborationdiscount.

Read More
Application
Western blot
Sample
Mouse Tissue lysate - whole (Muscle)
Gel Running Conditions
Reduced Denaturing (4-20% gel)
Loading amount
20 µg
Specification
Muscle
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C

Abcam user community

Verified customer

Submitted Nov 01 2011

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