Key features and details
- Rabbit polyclonal to MMAB
- Suitable for: IHC-P, WB, ICC/IF
- Reacts with: Human
- Isotype: IgG
Product nameAnti-MMAB antibody
See all MMAB primary antibodies
DescriptionRabbit polyclonal to MMAB
Tested applicationsSuitable for: IHC-P, WB, ICC/IFmore details
Species reactivityReacts with: Human
Predicted to work with: Mouse, Cow
- IHC-P: Human liver tissue. ICC/IF: A431 cells. WB: RT4 and U-251 MG cell lysate; Human liver tissue lysate.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.20
Preservative: 0.02% Sodium azide
Constituents: 40% Glycerol, PBS
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab243596 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||1/500 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.|
|WB||Use a concentration of 0.04 - 0.4 µg/ml.|
|ICC/IF||Use a concentration of 0.25 - 2 µg/ml.|
Tissue specificityExpressed in liver and skeletal muscle.
PathwayCofactor biosynthesis; adenosylcobalamin biosynthesis; adenosylcobalamin from cob(II)yrinate a,c-diamide: step 2/7.
Involvement in diseaseDefects in MMAB are the cause of methylmalonic aciduria type cblB (MMAB) [MIM:251110]; also known as methylmalonic aciduria type B or vitamin B12-responsive methylmalonicaciduria of cblB complementation type. MMAB is a disorder of methylmalonate and cobalamin metabolism due to defective synthesis of adenosylcobalamin. Inheritance is autosomal recessive.
Sequence similaritiesBelongs to the Cob(I)alamin adenosyltransferase family.
- Information by UniProt
- aquocob(I)alamin vitamin B12s adenosyltransferase antibody
- ATP:cob(I)alamin adenosyltransferase antibody
- ATP:corrinoid adenosyltransferase antibody
Formalin-fixed, paraffin-embedded human liver tissue stained for MMAB using ab243596 at 1/500 dilution in immunohistochemical analysis.
Paraformaldehyde-fixed, Triton X-100 permeabilized A431 (human epidermoid carcinoma cell line) cells labeling MMAB (Green) using ab243596 at 4 µg/mL in ICC/IF analysis.
All lanes : Anti-MMAB antibody (ab243596) at 0.4 µg/ml
Lane 1 : RT (Human urinary bladder cancer cell line) whole cell lysate
Lane 2 : U-251 MG (Human brain glioma cell line) whole cell lysate
Lane 3 : Human plasma (IgG/HSA depleted)
Lane 4 : Human liver tissue lysate
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab243596 has not yet been referenced specifically in any publications.