Recombinant Anti-MMAB antibody [EPR8695] (ab174831)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR8695] to MMAB
- Suitable for: WB, ICC/IF
- Reacts with: Human
Related conjugates and formulations
Overview
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Product name
Anti-MMAB antibody [EPR8695]
See all MMAB primary antibodies -
Description
Rabbit monoclonal [EPR8695] to MMAB -
Host species
Rabbit -
Tested applications
Suitable for: WB, ICC/IFmore details
Unsuitable for: Flow Cyt,IHC-P or IP -
Species reactivity
Reacts with: Human
Predicted to work with: RatDoes not react with: Mouse -
Immunogen
Synthetic peptide within Human MMAB aa 1-100 (Cysteine residue). The exact sequence is proprietary.
Database link: Q96EY8 -
Positive control
- HepG2, A673 and Human fetal liver lysates; HepG2 cells.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant -
Concentration information loading...
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Purity
Tissue culture supernatant -
Clonality
Monoclonal -
Clone number
EPR8695 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Isotype control
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Positive Controls
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Recombinant Protein
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Related Products
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab174831 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
WB |
1/1000 - 1/10000. Predicted molecular weight: 27 kDa.
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ICC/IF |
1/50 - 1/100.
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Notes |
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WB
1/1000 - 1/10000. Predicted molecular weight: 27 kDa. |
ICC/IF
1/50 - 1/100. |
Target
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Tissue specificity
Expressed in liver and skeletal muscle. -
Pathway
Cofactor biosynthesis; adenosylcobalamin biosynthesis; adenosylcobalamin from cob(II)yrinate a,c-diamide: step 2/7. -
Involvement in disease
Defects in MMAB are the cause of methylmalonic aciduria type cblB (MMAB) [MIM:251110]; also known as methylmalonic aciduria type B or vitamin B12-responsive methylmalonicaciduria of cblB complementation type. MMAB is a disorder of methylmalonate and cobalamin metabolism due to defective synthesis of adenosylcobalamin. Inheritance is autosomal recessive. -
Sequence similarities
Belongs to the Cob(I)alamin adenosyltransferase family. -
Cellular localization
Mitochondrion. - Information by UniProt
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Database links
- Entrez Gene: 326625 Human
- Entrez Gene: 687861 Rat
- Omim: 607568 Human
- SwissProt: Q96EY8 Human
- Unigene: 12106 Human
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Alternative names
- aquocob(I)alamin vitamin B12s adenosyltransferase antibody
- ATP:cob(I)alamin adenosyltransferase antibody
- ATP:corrinoid adenosyltransferase antibody
see all
Images
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All lanes : Anti-MMAB antibody [EPR8695] (ab174831) at 1/1000 dilution
Lane 1 : HepG2 cell lysate
Lane 2 : Human fetal liver tissue lysate
Lane 3 : A673 cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat anti-rabbit HRP at 1/2000 dilution
Developed using the ECL technique.
Predicted band size: 27 kDa -
Immunofluorescent analysis of HepG2 cells, labeling MMAB with ab174831 at 1/50 dilution (green). DAPI nuclear staining (blue).
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab174831 has not yet been referenced specifically in any publications.