Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR8695] to MMAB
- Suitable for: WB, ICC/IF
- Reacts with: Rat, Human
Product nameAnti-MMAB antibody [EPR8695]
See all MMAB primary antibodies
DescriptionRabbit monoclonal [EPR8695] to MMAB
Tested applicationsSuitable for: WB, ICC/IFmore details
Unsuitable for: Flow Cyt,IHC-P or IP
Species reactivityReacts with: Rat, Human
Does not react with: Mouse
Synthetic peptide within Human MMAB aa 1-100 (Cysteine residue). The exact sequence is proprietary.
Database link: Q96EY8
- HepG2, A673 and Human fetal liver lysates; HepG2 cells.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle.
Storage bufferpH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol, 0.05% BSA, 50% Tissue culture supernatant
Concentration information loading...
PurityTissue culture supernatant
Our Abpromise guarantee covers the use of ab174831 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/1000 - 1/10000. Predicted molecular weight: 27 kDa.|
|ICC/IF||1/50 - 1/100.|
Tissue specificityExpressed in liver and skeletal muscle.
PathwayCofactor biosynthesis; adenosylcobalamin biosynthesis; adenosylcobalamin from cob(II)yrinate a,c-diamide: step 2/7.
Involvement in diseaseDefects in MMAB are the cause of methylmalonic aciduria type cblB (MMAB) [MIM:251110]; also known as methylmalonic aciduria type B or vitamin B12-responsive methylmalonicaciduria of cblB complementation type. MMAB is a disorder of methylmalonate and cobalamin metabolism due to defective synthesis of adenosylcobalamin. Inheritance is autosomal recessive.
Sequence similaritiesBelongs to the Cob(I)alamin adenosyltransferase family.
- Information by UniProt
- aquocob(I)alamin vitamin B12s adenosyltransferase antibody
- ATP:cob(I)alamin adenosyltransferase antibody
- ATP:corrinoid adenosyltransferase antibody
All lanes : Anti-MMAB antibody [EPR8695] (ab174831) at 1/1000 dilution
Lane 1 : HepG2 cell lysate
Lane 2 : Human fetal liver lysate
Lane 3 : A673 cell lysate
Lysates/proteins at 10 µg per lane.
All lanes : Goat anti-rabbit HRP at 1/2000 dilution
Developed using the ECL technique.
Predicted band size: 27 kDa
Immunofluorescent analysis of HepG2 cells, labeling MMAB with ab174831 at 1/50 dilution (green). DAPI nuclear staining (blue).
ab174831 has not yet been referenced specifically in any publications.