Recombinant
RabMAb

Anti-MMAB antibody [EPR8695] (ab174831)

Overview

  • Product name
    Anti-MMAB antibody [EPR8695]
    See all MMAB primary antibodies
  • Description
    Rabbit monoclonal [EPR8695] to MMAB
  • Host species
    Rabbit
  • Tested applications
    Suitable for: WB, ICC/IFmore details
    Unsuitable for: Flow Cyt,IHC-P or IP
  • Species reactivity
    Reacts with: Rat, Human
    Does not react with: Mouse
  • Immunogen

    Synthetic peptide within Human MMAB aa 1-100 (Cysteine residue). The exact sequence is proprietary.
    Database link: Q96EY8

  • Positive control
    • HepG2, A673 and Human fetal liver lysates; HepG2 cells.
  • General notes

     

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab174831 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Predicted molecular weight: 27 kDa.
ICC/IF 1/50 - 1/100.
  • Application notes
    Is unsuitable for Flow Cyt,IHC-P or IP.
  • Target

    • Tissue specificity
      Expressed in liver and skeletal muscle.
    • Pathway
      Cofactor biosynthesis; adenosylcobalamin biosynthesis; adenosylcobalamin from cob(II)yrinate a,c-diamide: step 2/7.
    • Involvement in disease
      Defects in MMAB are the cause of methylmalonic aciduria type cblB (MMAB) [MIM:251110]; also known as methylmalonic aciduria type B or vitamin B12-responsive methylmalonicaciduria of cblB complementation type. MMAB is a disorder of methylmalonate and cobalamin metabolism due to defective synthesis of adenosylcobalamin. Inheritance is autosomal recessive.
    • Sequence similarities
      Belongs to the Cob(I)alamin adenosyltransferase family.
    • Cellular localization
      Mitochondrion.
    • Information by UniProt
    • Database links
    • Alternative names
      • aquocob(I)alamin vitamin B12s adenosyltransferase antibody
      • ATP:cob(I)alamin adenosyltransferase antibody
      • ATP:corrinoid adenosyltransferase antibody
      • ATR antibody
      • c-diamide adenosyltransferase antibody
      • cblB antibody
      • Cob antibody
      • Cob(I)alamin adenosyltransferase antibody
      • Cob(I)yrinic acid a antibody
      • cob(I)yrinic acid a c diamide adenosyltransferase mitochondrial antibody
      • Methylmalonic aciduria (cobalamin deficiency) cblB type antibody
      • Methylmalonic aciduria type B protein antibody
      • MGC20496 antibody
      • mitochondrial antibody
      • MMAB antibody
      • MMAB gene antibody
      • MMAB_HUMAN antibody
      • OTTHUMP00000240563 antibody
      • OTTHUMP00000240564 antibody
      see all

    Images

    • All lanes : Anti-MMAB antibody [EPR8695] (ab174831) at 1/1000 dilution

      Lane 1 : HepG2 cell lysate
      Lane 2 : Human fetal liver lysate
      Lane 3 : A673 cell lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : Goat anti-rabbit HRP at 1/2000 dilution

      Developed using the ECL technique.

      Predicted band size: 27 kDa

    • Immunofluorescent analysis of HepG2 cells, labeling MMAB with ab174831 at 1/50 dilution (green). DAPI nuclear staining (blue).

    References

    ab174831 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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