Key features and details
- Mouse monoclonal [OTI2G5] to MMAB
- Suitable for: WB, IHC-P, Flow Cyt
- Reacts with: Human
- Isotype: IgG1
Product nameAnti-MMAB antibody [OTI2G5]
See all MMAB primary antibodies
DescriptionMouse monoclonal [OTI2G5] to MMAB
Tested applicationsSuitable for: WB, IHC-P, Flow Cytmore details
Species reactivityReacts with: Human
Recombinant full length protein corresponding to Human MMAB aa 33-250. (NP_443077) produced in HEK293T cell.
QSRGPQGVEDGDRPQPSSKTPRIPKIYTKTGDKGFSSTFTGERRPKDDQV FEAVGTTDELSSAIGFALELVTEKGHTFAEELQKIQCTLQDVGSALATPC SSAREAHLKYTTFKAGPILELEQWIDKYTSQLPPLTAFILPSGGKISSAL HFCRAVCRRAERRVVPLVQMGETDANVAKFLNRLSDYLFTLARYAAMKEG NQEKIYMKNDPSAESEGL
Database link: Q96EY8
- WB: HEK-293T cells transfected with pCMV6-ENTRY MMAB IHC-P: Human Kidney tissue, carcinoma of Human kidney tissue, Human liver tissue, carcinoma of Human prostate tissue, Human lymph node tissue Flow Cyt: Jurkat cells and transfected HEK-293T cells
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In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
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Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.30
Preservative: 0.02% Sodium azide
Constituents: PBS, 1% BSA, 50% Glycerol
Concentration information loading...
Purification notesPurified from cell culture supernatant by affinity chromatography
Our Abpromise guarantee covers the use of ab123916 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/2000. Predicted molecular weight: 27 kDa.|
ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.
Tissue specificityExpressed in liver and skeletal muscle.
PathwayCofactor biosynthesis; adenosylcobalamin biosynthesis; adenosylcobalamin from cob(II)yrinate a,c-diamide: step 2/7.
Involvement in diseaseDefects in MMAB are the cause of methylmalonic aciduria type cblB (MMAB) [MIM:251110]; also known as methylmalonic aciduria type B or vitamin B12-responsive methylmalonicaciduria of cblB complementation type. MMAB is a disorder of methylmalonate and cobalamin metabolism due to defective synthesis of adenosylcobalamin. Inheritance is autosomal recessive.
Sequence similaritiesBelongs to the Cob(I)alamin adenosyltransferase family.
- Information by UniProt
- aquocob(I)alamin vitamin B12s adenosyltransferase antibody
- ATP:cob(I)alamin adenosyltransferase antibody
- ATP:corrinoid adenosyltransferase antibody
All lanes : Anti-MMAB antibody [OTI2G5] (ab123916) at 1/2000 dilution
Lane 1 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) cell lysate transfected with pCMV6-ENTRY control
Lane 2 : HEK-293T cells transfected with pCMV6-ENTRY MMAB
Lysates/proteins at 5 µg per lane.
Predicted band size: 27 kDa
Paraffin-embedded human kidney tissue stained for MMAB using ab123916 at 1/150 dilution in immunohistochemical analysis.
Paraffin-embedded human kidney carcinoma tissue stained for MMAB using ab123916 at 1/150 dilution in immunohistochemical analysis.
Paraffin-embedded human liver tissue stained for MMAB using ab123916 at 1/150 dilution in immunohistochemical analysis.
Paraffin-embedded human prostate carcinoma tissue stained for MMAB using ab123916 at 1/150 dilution in immunohistochemical analysis.
Paraffin-embedded human lymph node tissue stained for MMAB using ab123916 at 1/150 dilution in immunohistochemical analysis.
Flow cytometric analysis of HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) cells with either pCMV6-ENTRY MMAB (Red), or pCMV6-ENTRY control plasmid (Blue), stained using ab123916 at 1/100 dilution.
Flow cytometric analysis of jurkat (human T cell leukemia cell line from peripheral blood) cells stained for MMAB (Red) using ab123916 at 1/100 dilution, compared to a nonspecific negative control antibody (Blue).
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab123916 has not yet been referenced specifically in any publications.