Anti-MMACHC/CblC antibody (ab96195)
Key features and details
- Rabbit polyclonal to MMACHC/CblC
- Suitable for: WB, ICC/IF
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-MMACHC/CblC antibody
See all MMACHC/CblC primary antibodies -
Description
Rabbit polyclonal to MMACHC/CblC -
Host species
Rabbit -
Tested applications
Suitable for: WB, ICC/IFmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat, Cow -
Immunogen
Recombinant fragment corresponding to Human MMACHC/CblC aa 1-188.
Database link: NP_056321 -
Positive control
- 293T and Raji whole cell lysates; HeLa cells; A431, H1299, HeLaS3 and HepG2 cell lines
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
Storage buffer
pH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 1.21% Tris, 0.75% Glycine, 10% Glycerol (glycerin, glycerine) -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab96195 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
1/500 - 1/3000. Predicted molecular weight: 32 kDa.
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ICC/IF |
1/100 - 1/200.
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Notes |
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WB
1/500 - 1/3000. Predicted molecular weight: 32 kDa. |
ICC/IF
1/100 - 1/200. |
Target
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Function
May be involved in the binding and intracellular trafficking of cobalamin (vitamin B12). -
Tissue specificity
Widely expressed. Expressed at higher level in fetal liver. Also expressed in spleen, lymph node, thymus and bone marrow. Weakly or not expressed in peripheral blood leukocytes. -
Pathway
Cofactor biosynthesis; adenosylcobalamin biosynthesis. -
Involvement in disease
Defects in MMACHC are the cause of methylmalonic aciduria and homocystinuria type cblC (MMACHC) [MIM:277400]. MMACHC is a disorder of cobalamin metabolism characterized by decreased levels of the coenzymes adenosylcobalamin (AdoCbl) and methylcobalamin (MeCbl). Affected individuals may have developmental, hematologic, neurologic, metabolic, ophthalmologic, and dermatologic clinical findings. Although considered a disease of infancy or childhood, some individuals develop symptoms in adulthood. -
Sequence similarities
Belongs to the MMACHC family. - Information by UniProt
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Database links
- Entrez Gene: 513433 Cow
- Entrez Gene: 25974 Human
- Entrez Gene: 67096 Mouse
- Entrez Gene: 313520 Rat
- Omim: 609831 Human
- SwissProt: Q5E9C8 Cow
- SwissProt: Q9Y4U1 Human
- SwissProt: Q9CZD0 Mouse
see all -
Alternative names
- 1810037K07Rik antibody
- BOS_3654 antibody
- cblC antibody
see all
Images
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All lanes : Anti-MMACHC/CblC antibody (ab96195) at 1/1000 dilution
Lane 1 : 293T whole cell lysate
Lane 2 : Raji whole cell lysate
Lysates/proteins at 30 µg per lane.
Predicted band size: 32 kDa
12% SDS-PAGE -
ab96195, at 1/100 dilution, staining MMACHC/CblC in paraformaldehyde-fixed HeLa cells by Immunofluorescence. Lower image is merged with DNA probe.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (2)
ab96195 has been referenced in 2 publications.
- Fofou-Caillierez MB et al. Interaction between methionine synthase isoforms and MMACHC: characterization in cblG-variant, cblG and cblC inherited causes of megaloblastic anaemia. Hum Mol Genet N/A:N/A (2013). PubMed: 23825108
- Yu HC et al. An X-linked cobalamin disorder caused by mutations in transcriptional coregulator HCFC1. Am J Hum Genet 93:506-14 (2013). PubMed: 24011988