Key features and details
- Rabbit polyclonal to MMACHC/CblC
- Suitable for: WB, ICC/IF
- Reacts with: Human
- Isotype: IgG
Product nameAnti-MMACHC/CblC antibody
See all MMACHC/CblC primary antibodies
DescriptionRabbit polyclonal to MMACHC/CblC
Tested applicationsSuitable for: WB, ICC/IFmore details
Species reactivityReacts with: Human
Predicted to work with: Mouse, Rat, Cow
Recombinant fragment corresponding to Human MMACHC/CblC aa 1-188.
Database link: NP_056321
- 293T and Raji whole cell lysates; HeLa cells; A431, H1299, HeLaS3 and HepG2 cell lines
This product was previously labelled as MMACHC
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We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
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Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage bufferpH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 1.21% Tris, 0.75% Glycine, 10% Glycerol (glycerin, glycerine)
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab96195 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/500 - 1/3000. Predicted molecular weight: 32 kDa.|
|ICC/IF||1/100 - 1/200.|
FunctionMay be involved in the binding and intracellular trafficking of cobalamin (vitamin B12).
Tissue specificityWidely expressed. Expressed at higher level in fetal liver. Also expressed in spleen, lymph node, thymus and bone marrow. Weakly or not expressed in peripheral blood leukocytes.
PathwayCofactor biosynthesis; adenosylcobalamin biosynthesis.
Involvement in diseaseDefects in MMACHC are the cause of methylmalonic aciduria and homocystinuria type cblC (MMACHC) [MIM:277400]. MMACHC is a disorder of cobalamin metabolism characterized by decreased levels of the coenzymes adenosylcobalamin (AdoCbl) and methylcobalamin (MeCbl). Affected individuals may have developmental, hematologic, neurologic, metabolic, ophthalmologic, and dermatologic clinical findings. Although considered a disease of infancy or childhood, some individuals develop symptoms in adulthood.
Sequence similaritiesBelongs to the MMACHC family.
- Information by UniProt
- 1810037K07Rik antibody
- BOS_3654 antibody
- cblC antibody
All lanes : Anti-MMACHC/CblC antibody (ab96195) at 1/1000 dilution
Lane 1 : 293T whole cell lysate
Lane 2 : Raji whole cell lysate
Lysates/proteins at 30 µg per lane.
Predicted band size: 32 kDa
ab96195, at 1/100 dilution, staining MMACHC/CblC in paraformaldehyde-fixed HeLa cells by Immunofluorescence. Lower image is merged with DNA probe.
ab96195 has been referenced in 2 publications.
- Fofou-Caillierez MB et al. Interaction between methionine synthase isoforms and MMACHC: characterization in cblG-variant, cblG and cblC inherited causes of megaloblastic anaemia. Hum Mol Genet N/A:N/A (2013). PubMed: 23825108
- Yu HC et al. An X-linked cobalamin disorder caused by mutations in transcriptional coregulator HCFC1. Am J Hum Genet 93:506-14 (2013). PubMed: 24011988