Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR11268] to MMACHC/CblC
- Suitable for: WB, IP
- Reacts with: Rat, Human
Product nameAnti-MMACHC/CblC antibody [EPR11268]
See all MMACHC/CblC primary antibodies
DescriptionRabbit monoclonal [EPR11268] to MMACHC/CblC
Tested applicationsSuitable for: WB, IPmore details
Unsuitable for: Flow Cyt,ICC/IF or IHC-P
Species reactivityReacts with: Rat, Human
within Human MMACHC/CblC aa 250 to the C-terminus (Cysteine residue). The exact sequence is proprietary.
Database link: Q9Y4U1
- HepG2, U937 and Raji cell lysates.
Mouse: We have preliminary internal testing data to indicate this antibody may not react with this species. Please contact us for more information.
This product was previously labelled as MMACHC
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant
Concentration information loading...
PurityTissue culture supernatant
Our Abpromise guarantee covers the use of ab177956 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/10000 - 1/50000. Predicted molecular weight: 32 kDa.|
|IP||1/10 - 1/100.|
FunctionMay be involved in the binding and intracellular trafficking of cobalamin (vitamin B12).
Tissue specificityWidely expressed. Expressed at higher level in fetal liver. Also expressed in spleen, lymph node, thymus and bone marrow. Weakly or not expressed in peripheral blood leukocytes.
PathwayCofactor biosynthesis; adenosylcobalamin biosynthesis.
Involvement in diseaseDefects in MMACHC are the cause of methylmalonic aciduria and homocystinuria type cblC (MMACHC) [MIM:277400]. MMACHC is a disorder of cobalamin metabolism characterized by decreased levels of the coenzymes adenosylcobalamin (AdoCbl) and methylcobalamin (MeCbl). Affected individuals may have developmental, hematologic, neurologic, metabolic, ophthalmologic, and dermatologic clinical findings. Although considered a disease of infancy or childhood, some individuals develop symptoms in adulthood.
Sequence similaritiesBelongs to the MMACHC family.
- Information by UniProt
- 1810037K07Rik antibody
- BOS_3654 antibody
- cblC antibody
Western blot analysis on immunoprecipitation pellet from HepG2 cell lysate using ab177956.
All lanes : Anti-MMACHC/CblC antibody [EPR11268] (ab177956) at 1/10000 dilution
Lane 1 : HepG2 cell lysate
Lane 2 : U937 cell lysate
Lane 3 : Raji cell lysate
Lysates/proteins at 10 µg per lane.
Predicted band size: 32 kDa
ab177956 has not yet been referenced specifically in any publications.