Recombinant
RabMAb

Recombinant Anti-MMACHC/CblC antibody [EPR11268] (ab177956)

Overview

  • Product name

    Anti-MMACHC/CblC antibody [EPR11268]
    See all MMACHC/CblC primary antibodies
  • Description

    Rabbit monoclonal [EPR11268] to MMACHC/CblC
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IPmore details
    Unsuitable for: Flow Cyt,ICC/IF or IHC-P
  • Species reactivity

    Reacts with: Rat, Human
  • Immunogen

    within Human MMACHC/CblC aa 250 to the C-terminus (Cysteine residue). The exact sequence is proprietary.
    Database link: Q9Y4U1

  • Positive control

    • HepG2, U937 and Raji cell lysates.
  • General notes

    Mouse: We have preliminary internal testing data to indicate this antibody may not react with this species. Please contact us for more information.

    This product was previously labelled as MMACHC

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab177956 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/10000 - 1/50000. Predicted molecular weight: 32 kDa.
IP 1/10 - 1/100.
  • Application notes
    Is unsuitable for Flow Cyt,ICC/IF or IHC-P.
  • Target

    • Function

      May be involved in the binding and intracellular trafficking of cobalamin (vitamin B12).
    • Tissue specificity

      Widely expressed. Expressed at higher level in fetal liver. Also expressed in spleen, lymph node, thymus and bone marrow. Weakly or not expressed in peripheral blood leukocytes.
    • Pathway

      Cofactor biosynthesis; adenosylcobalamin biosynthesis.
    • Involvement in disease

      Defects in MMACHC are the cause of methylmalonic aciduria and homocystinuria type cblC (MMACHC) [MIM:277400]. MMACHC is a disorder of cobalamin metabolism characterized by decreased levels of the coenzymes adenosylcobalamin (AdoCbl) and methylcobalamin (MeCbl). Affected individuals may have developmental, hematologic, neurologic, metabolic, ophthalmologic, and dermatologic clinical findings. Although considered a disease of infancy or childhood, some individuals develop symptoms in adulthood.
    • Sequence similarities

      Belongs to the MMACHC family.
    • Information by UniProt
    • Database links

    • Alternative names

      • 1810037K07Rik antibody
      • BOS_3654 antibody
      • cblC antibody
      • DKFZp564I122 antibody
      • FLJ25671 antibody
      • Methylmalonic aciduria (cobalamin deficiency) cblC type with homocystinuria antibody
      • Methylmalonic aciduria and homocystinuria type C protein antibody
      • Methylmalonic aciduria and homocystinuria type C protein homolog antibody
      • MGC134307 antibody
      • MMAC_HUMAN antibody
      • MMACHC antibody
      • OTTHUMP00000009243 antibody
      • RP11 291L19.3 antibody
      • RP23-177C18.3 antibody
      see all

    Images

    • Western blot analysis on immunoprecipitation pellet from HepG2 cell lysate using ab177956.

    • All lanes : Anti-MMACHC/CblC antibody [EPR11268] (ab177956) at 1/10000 dilution

      Lane 1 : HepG2 cell lysate
      Lane 2 : U937 cell lysate
      Lane 3 : Raji cell lysate

      Lysates/proteins at 10 µg per lane.

      Predicted band size: 32 kDa

    References

    ab177956 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

    There are currently no Customer reviews or Questions for ab177956.
    Please use the links above to contact us or submit feedback about this product.

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
    For licensing inquiries, please contact partnerships@abcam.com

    Sign up