Overview

  • Product name

    Anti-mMCP-4 antibody
  • Description

    Goat polyclonal to mMCP-4
  • Host species

    Goat
  • Tested applications

    Suitable for: IHC-Pmore details
  • Species reactivity


    Predicted to work with: Mouse
  • Immunogen

    Synthetic peptide corresponding to Mouse mMCP-4 aa 114-126 (internal sequence) (Cysteine residue).
    Sequence:

    KLQKKAKETPSVN

  • General notes

    Previously labelled as MCPT4. 

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
  • Storage buffer

    Preservative: 0.02% Sodium Azide
    Constituents: 0.5% BSA, Tris buffered saline, pH 7.3
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Purification notes

    ab92368 is purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab92368 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P Use at an assay dependent concentration.

Target

  • Function

    Has chymotrypsin-like activity. Hydrolyzes the amide bonds of synthetic substrates having Tyr and Phe residues at the P1 position. Preferentially hydrolyzes the 'Tyr-4-
    -Ile-5' bond of angiotensin I and the 'Phe-20-
    -Ala-21' bond of amyloid beta-protein, and is less active towards the 'Phe-8-
    -His-9' bond of angiotensin I and the 'Phe-4-
    -Ala-5' and 'Tyr-10-
    -Glu-11' bonds of amyloid beta-protein. Involved in thrombin regulation and fibronectin processing.
  • Tissue specificity

    Submucosal mast cells. In femoral muscle, detected in myocytes but not in mast cells.
  • Sequence similarities

    Belongs to the peptidase S1 family. Granzyme subfamily.
    Contains 1 peptidase S1 domain.
  • Information by UniProt
  • Database links

  • Alternative names

    • Mast cell protease 4 antibody
    • Mcp 4 antibody
    • Mcpt4 antibody
    • MCPT4_MOUSE antibody
    • MMCP 4 antibody
    • MMCP 4A antibody
    • MMCP 4B antibody
    • MMCP-4 antibody
    • MMCP4 antibody
    • MMCP4A antibody
    • MMCP4B antibody
    • MSMCP antibody
    • Myonase antibody
    • Serosal mast cell protease antibody
    see all

Images

  • Immunohistochemical staining of paraffin embedded Mouse Skeletal Muscle with ab92368 (3.75 µg/ml) . Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

References

This product has been referenced in:

  • Rowbotham SP  et al. H3K9 methyltransferases and demethylases control lung tumor-propagating cells and lung cancer progression. Nat Commun 9:4559 (2018). Read more (PubMed: 30455465) »
See 1 Publication for this product

Customer reviews and Q&As

1-4 of 4 Abreviews or Q&A

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Blocking step
Serum as blocking agent for 30 minute(s) · Concentration: 10% · Temperature: 25°C
Antigen retrieval step
Heat mediated
Sample
Mouse Tissue sections (Heart)
Specification
Heart
Permeabilization
No
Fixative
10% Formalin

Abcam user community

Verified customer

Submitted Aug 26 2014

Answer

Thank you for contacting us.

Please be aware that our ELISA assay is used for determining the activity of the antibody by applying serially diluted antibody to micro-wells that are coated with the immunizing peptide.

ELISA titre determination protcol:

Wells are coated with the immunizing peptide and serially diluted antibodies are applied and measured.

Coat 0.1ug peptide in 100ul PBS per well on high binding ELISA plates overnight at 4C.
Wash 3 times with 300ul PBS per well.
Block with PBS/0.1%(v/v) Tween-20 (PBST) containing 3%(w/v) skimmed milk (blocking buffer) for 1h at 37C
Wash 3 times with PBST
Make serially dilutions of antibody in blocking buffer and apply 100ul per well.
Incubate 1h at 37C with the plates covered
Wash three times with 300ul PBST
Apply secondary antibody (AP-conjugate) at appropriate dilution in blocking buffer, 100ul per well
Wash four times in 300ul PBST
Wash 2 times in 300ul PBS
Add 50ul per well p-nitro phenyl phosphate (pNPP) from Sigma and incubate for 30min at 30C
Stop the reaction with 50ul 1M NaOH.
Read at 405nm



Phosphate buffered saline (PBS): 20mM Sodium phosphate, pH7.4 in 150mM NaCl.

I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

Use our products? Submit an Abreview. Earn rewards!
https://www.abcam.com/abreviews

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Answer

Thank you for contacting us.

ab92368 is a Fast Track antibody. Fast-Track antibodies constitute a diverse group of products that have undergone variable degrees of characterization. They have all been affinity purified and show high titre values against the immunizing peptide (by ELISA). This antibody has been characterized initially in peptide ELISA i.e. the immunizing peptide was coated on the plate. The antibody detection limit is 1/64,000.

We do not have any ELISA data with other samples or even with natural endogenous protein; which is why we are selling this product at cheap price. Please read the full terms of use given on the datasheet.

This product is not covered under Abpromise guarantee because the return you will get from Abpoints will be approximately equal to the price you pay for this product.

I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

Use our products? Submit an Abreview. Earn rewards!
https://www.abcam.com/abreviews

Read More

Answer

Thank you for contacting us regarding ab92368. As you may know, ab92368 is one of our Fast-track antibodies and it is not yet fully characterized. We cannot guarantee that the antibody will work in any application other than ELISA against the immunizing peptide and are therefore unable to offer a replacement, credit or refund if the antibody does not work in your application. Due to this, Fast-Track antibodies are offered at a reduced price.  We do not have a small aliquot sample size of our products simply because we carry over 70,000 items and wouldn't have space in our automated freezers. If you decide to purchase this antibody, we would greatly appreciate your feedback on this antibody, whether positive or negative. We have a special feedback reward system for Fast-track products: we will award 950 Abpoints to the first researcher who sends us positive feedback (including an image and details of your protocol). Conclusive negative feedback that leads us to withdraw the antibody will also be rewarded with 950 Abpoints. Other useful positive and negative feedback will be rewarded with the standard amount of Abpoints for submitting an Abreview (please refer to Abreviews terms and conditions for more detailed information, see https://www.abcam.com/abreviews). All awards are subject to approval by Abcam scientists and provision of suitable data. Please submit feedback to fast-track@abcam.com. I hope this information has been helpful to you. We greatly appreciate any feedback you can provide.  

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