Anti-MMGT1 antibody - C-terminal (ab174366)

Overview

  • Product name
    Anti-MMGT1 antibody - C-terminal
    See all MMGT1 primary antibodies
  • Description
    Rabbit polyclonal to MMGT1 - C-terminal
  • Host species
    Rabbit
  • Tested applications
    Suitable for: Flow Cyt, IHC-P, WBmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Cow
  • Immunogen

    Synthetic peptide within Human MMGT1 aa 102-131 (C terminal) conjugated to Keyhole Limpet Haemocyanin (KLH). The exact sequence is proprietary.
    Database link: Q8N4V1

  • Positive control
    • HeLa cells and lysate; Human brain tissue.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer
    Preservative: 0.09% Sodium azide
    Constituent: 99% PBS
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Purification notes
    ab174366 is purified through a protein A column, followed by peptide affinity purification.
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab174366 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt 1/10 - 1/50.

ab171870 - Rabbit polyclonal IgG, is suitable for use as an isotype control with this antibody.

 

IHC-P 1/50 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
WB 1/100 - 1/500. Predicted molecular weight: 15 kDa.

Target

  • Function
    Mediates Mg(2+) transport.
  • Sequence similarities
    Belongs to the membrane magnesium transporter family.
  • Cellular localization
    Endoplasmic reticulum membrane. Golgi apparatus membrane. Early endosome membrane. Detected in the Golgi apparatus and in post-Golgi vesicles including early endosomes.
  • Information by UniProt
  • Database links
  • Alternative names
    • 9630048L06Rik antibody
    • BC032271 antibody
    • EMC5 antibody
    • Membrane magnesium transporter 1 antibody
    • Mmgt1 antibody
    • MMGT1_HUMAN antibody
    • RGD1566339 antibody
    • RP11-274K13.3 antibody
    • TMEM32 antibody
    • Transmembrane protein 32 antibody
    see all

Images

  • Immunohistochemistry analysis in formalin fixed and paraffin embedded Human brain tissue labeling MMGT1 with ab174366 at 1/50 dilution, followed by peroxidase conjugation of the secondary antibody and DAB staining.

  • Anti-MMGT1 antibody - C-terminal (ab174366) at 1/100 dilution + HeLa cell lysates at 35 µg

    Predicted band size: 15 kDa

  • Flow cytometric analysis of Hela cells labeling MMGT1 with ab174366 at 1/10 dilution (right histogram) compared to a negative control cell (left histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

References

ab174366 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab174366.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

Sign up