Key features and details
- Assay type: Enzyme activity
- Detection method: Fluorescent
- Platform: Microplate reader
- Assay time: 1 hr
- Sample type: Cell culture media, Cell culture supernatant, Milk, Tissue Extracts, Urine
- Sensitivity: 0.05 mU/well
Product nameMMP-3 Activity Assay Kit (Fluorometric)
See all MMP3 kits
Sample typeCell culture supernatant, Milk, Urine, Tissue Extracts, Cell culture media
Assay typeEnzyme activity
Sensitivity< 0.05 mU/well
Assay time1h 00m
Species reactivityReacts with: Mammals, Other species
In Abcam's MMP-3 Activity Assay Kit, MMP-3 hydrolyzes a specific FRET substrate to release the quenched fluorescent group Mca, which can be detected fluorometrically at Ex/Em=325/393 nm. The kit provides a rapid, simple, sensitive and reliable test which can also be used as a high throughput assay of MMP-3 activity. The assay sensitivity is < 50 µU.
Visit our FAQs page for tips and troubleshooting.
The matrix metalloproteinase-3 (MMP-3, stromelysin-1) exhibits a number of activities that would make it a particularly good tumor promoter. Like several other MMPs, MMP-3 was first cloned and later recloned as a cancer-specific gene. In addition to degrading numerous extracellular matrix components, MMP-3 can activate gelatinase B, the collagenases and several serpin-type serine proteinase inhibitors. Moreover, it can release a number of cell surface molecules, including E-cadherin, a known contributor to cancer development.
Abcam has not and does not intend to apply for the REACH Authorisation of customers’ uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.
Storage instructionsStore at -20°C. Please refer to protocols.
Components Identifier 100 tests Mca Standard (1mM) Yellow 1 x 20µl MMP-3 Assay Buffer WM 1 x 25ml MMP-3 Positive Control (lyophilized) Green 1 vial MMP-3 Substrate Red 1 x 200µl
FunctionCan degrade fibronectin, laminin, gelatins of type I, III, IV, and V; collagens III, IV, X, and IX, and cartilage proteoglycans. Activates procollagenase.
Sequence similaritiesBelongs to the peptidase M10A family.
Contains 4 hemopexin-like domains.
DomainThe conserved cysteine present in the cysteine-switch motif binds the catalytic zinc ion, thus inhibiting the enzyme. The dissociation of the cysteine from the zinc ion upon the activation-peptide release activates the enzyme.
Cellular localizationSecreted > extracellular space > extracellular matrix.
- Information by UniProt
- Matrix metalloproteinase 3
- Matrix metalloproteinase 3 preproprotein
ab118972 has been referenced in 7 publications.
- Deng Y et al. Estradiol/GPER affects the integrity of mammary duct-like structures in vitro. Sci Rep 10:1386 (2020). PubMed: 31992771
- Hsiao YH et al. Phloretin suppresses metastasis by targeting protease and inhibits cancer stemness and angiogenesis in human cervical cancer cells. Phytomedicine 62:152964 (2019). PubMed: 31153059
- Choi J et al. Interplay between RNASEH2 and MOV10 controls LINE-1 retrotransposition. Nucleic Acids Res 46:1912-1926 (2018). PubMed: 29315404
- O'Callaghan J et al. Therapeutic potential of AAV-mediated MMP-3 secretion from corneal endothelium in treating glaucoma. Hum Mol Genet 26:1230-1246 (2017). PubMed: 28158775
- Pettersson F et al. Genetic and pharmacologic inhibition of eIF4E reduces breast cancer cell migration, invasion, and metastasis. Cancer Res 75:1102-12 (2015). PubMed: 25608710
- Sun H et al. Hyperglycemia-suppressed expression of Serpine1 contributes to delayed epithelial wound healing in diabetic mouse corneas. Invest Ophthalmol Vis Sci 56:3383-92 (2015). PubMed: 26024123
- Turturro S et al. Sustained release of matrix metalloproteinase-3 to trabecular meshwork cells using biodegradable PLGA microparticles. Mol Pharm 10:3023-3032 (2013). PubMed: 23795867