Overview

  • Product name
    MMP-3 Activity Assay Kit (Fluorometric)
    See all MMP3 kits
  • Detection method
    Fluorescent
  • Sample type
    Cell culture supernatant, Milk, Urine, Tissue Extracts, Cell culture media
  • Assay type
    Enzyme activity
  • Sensitivity
    < 0.05 mU/well
  • Assay time
    1h 00m
  • Species reactivity
    Reacts with: Mammals, Other species
  • Product overview

    In Abcam's MMP-3 Activity Assay Kit, MMP-3 hydrolyzes a specific FRET substrate to release the quenched fluorescent group Mca, which can be detected fluorometrically at Ex/Em=325/393 nm. The kit provides a rapid, simple, sensitive and reliable test which can also be used as a high throughput assay of MMP-3 activity. The assay sensitivity is < 50 µU.
    Visit our FAQs page for tips and troubleshooting.

  • Notes

    The matrix metalloproteinase-3 (MMP-3, stromelysin-1) exhibits a number of activities that would make it a particularly good tumor promoter. Like several other MMPs, MMP-3 was first cloned and later recloned as a cancer-specific gene. In addition to degrading numerous extracellular matrix components, MMP-3 can activate gelatinase B, the collagenases and several serpin-type serine proteinase inhibitors. Moreover, it can release a number of cell surface molecules, including E-cadherin, a known contributor to cancer development.

  • Platform
    Microplate reader

Properties

Images

  • MMP-3 Positive Control
  • MMP-3 Mca Standard

Protocols

References

This product has been referenced in:
  • Choi J  et al. Interplay between RNASEH2 and MOV10 controls LINE-1 retrotransposition. Nucleic Acids Res 46:1912-1926 (2018). Read more (PubMed: 29315404) »
  • O'Callaghan J  et al. Therapeutic potential of AAV-mediated MMP-3 secretion from corneal endothelium in treating glaucoma. Hum Mol Genet 26:1230-1246 (2017). Read more (PubMed: 28158775) »
See all 4 Publications for this product

Customer reviews and Q&As

1-5 of 5 Abreviews or Q&A

Answer

Thank you for contacting us.

Since ab118972 is an enzyme assay, we would not recommend EDTA treated plasma for it. The customer could try using heparin instead for plasma.

I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

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Answer

Thank you for contacting us.

The FRET substrate we provide in this kit is very specific for MMP-3. Other MMPs should not be able to hydrolyze it, therefore there should not be any interference of that sort.

I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

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Answer

Thank you for contacting us.

EDTA is known to inhibit MMP-3 activity in general conditions. However, we have not encountered this problem specifically. It might depend on the concentration of EDTA used. Nevertheless, it is always better to use this kit with EDTA-free sample.

I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

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Question
Answer

Thank you for contacting us.

The molecular weight of the Active MMP-3 is: 19.5 kDa.


I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

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Answer

Thank you for contacting us. Each vial of MMP-3 positive control contains 2.2 ug of the active protein that has been lyophilized. I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
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