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So in the kit there is the APMA that you incubate with the sample in order to activate the "pro" forms of the MMPs. Does this actually mean that I am measuring the "pro" and "actual" MMP activity? Or would the previously activated enzymes have degraded during the freezing or incubation process? Have you ever tested the assay with and without APMA incubation to see if there would still be some substrate cleavage due to only the previously activated MMPs?
Asked on Aug 30 2012
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The MMPs are initially synthesized as inactive Zymogens with a pro-peptide domain that must be removed before the enzymeis active. Some MMPs have a prohormone convertase cleavage site as part of this domain, which, when cleaved, activates the enzyme.
The kit is for measuring the activated MMPs activity. So if you add APMA, then you are measuring both activated and pro MMPs.
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Answered on Aug 30 2012