Anti-MMP1 antibody (ab137332)
Key features and details
- Rabbit polyclonal to MMP1
- Suitable for: ICC/IF, WB
- Reacts with: Mouse, Human
- Isotype: IgG
Get better batch-to-batch reproducibility with a recombinant antibody
- Research with confidence – consistent and reproducible results with every batch
- Long-term and scalable supply – powered by recombinant technology for fast production
- Success from the first experiment – confirmed specificity through extensive validation
- Ethical standards compliant – production is animal-free
Overview
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Product name
Anti-MMP1 antibody
See all MMP1 primary antibodies -
Description
Rabbit polyclonal to MMP1 -
Host species
Rabbit -
Tested applications
Suitable for: ICC/IF, WBmore details
Unsuitable for: IHC-P -
Species reactivity
Reacts with: Mouse, Human
Predicted to work with: Rabbit, Cow, Pig -
Immunogen
Recombinant fragment corresponding to Human MMP1 aa 42-244.
Database link: P03956 -
General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.00
Preservative: 0.025% Proclin 300
Constituents: 78% PBS, 1% BSA, 20% Glycerol (glycerin, glycerine) -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Assay kits
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Compatible Secondaries
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Isotype control
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Positive Controls
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab137332 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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ICC/IF | (1) |
Use at an assay dependent concentration. PubMed: 23902766
|
WB | (1) |
1/500 - 1/3000. Predicted molecular weight: 54 kDa.
Blocking in 5% non-fat milk in TBST for 60 minutes at room temperature. |
Notes |
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ICC/IF
Use at an assay dependent concentration. PubMed: 23902766 |
WB
1/500 - 1/3000. Predicted molecular weight: 54 kDa. Blocking in 5% non-fat milk in TBST for 60 minutes at room temperature. |
Target
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Function
Cleaves collagens of types I, II, and III at one site in the helical domain. Also cleaves collagens of types VII and X. In case of HIV infection, interacts and cleaves the secreted viral Tat protein, leading to a decrease in neuronal Tat's mediated neurotoxicity. -
Sequence similarities
Belongs to the peptidase M10A family.
Contains 4 hemopexin-like domains. -
Domain
There are two distinct domains in this protein; the catalytic N-terminal, and the C-terminal which is involved in substrate specificity and in binding TIMP (tissue inhibitor of metalloproteinases).
The conserved cysteine present in the cysteine-switch motif binds the catalytic zinc ion, thus inhibiting the enzyme. The dissociation of the cysteine from the zinc ion upon the activation-peptide release activates the enzyme. -
Post-translational
modificationsUndergoes autolytic cleavage to two major forms (22 kDa and 27 kDa). A minor form (25 kDa) is the glycosylated form of the 22 kDa form. The 27 kDa form has no activity while the 22/25 kDa form can act as activator for collagenase. -
Cellular localization
Secreted > extracellular space > extracellular matrix. - Information by UniProt
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Database links
- Entrez Gene: 4312 Human
- Entrez Gene: 100009110 Rabbit
- Omim: 120353 Human
- SwissProt: P28053 Cow
- SwissProt: P03956 Human
- SwissProt: P21692 Pig
- SwissProt: P13943 Rabbit
- Unigene: 83169 Human
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Alternative names
- 27 kDa interstitial collagenase antibody
- CLG antibody
- CLGN antibody
see all
Images
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Anti-MMP1 antibody (ab137332) at 1/2500 dilution + HUVEC (human umbilical vein endothelial cell line) whole cell extract at 30 µg
Secondary
HRP-conjugated anti-rabbit IgG at 1/10000 dilution
Predicted band size: 54 kDa10% SDS gel
Running condition: 80V, 15min; 140V, 40 min
Primary antibody incubation: 4C overnight
Washing condition: 5 mL TBST, 4 x 5 minutes -
Anti-MMP1 antibody (ab137332) at 1/1000 dilution + Raji (Human Burkitt's lymphoma cell line) whole cell lysate at 30 µg
Predicted band size: 54 kDa
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (61)
ab137332 has been referenced in 61 publications.
- Zhou C et al. Anxa1 in smooth muscle cells protects against acute aortic dissection. Cardiovasc Res 118:1564-1582 (2022). PubMed: 33757117
- Lohakul J et al. Mitochondria-Targeted Hydrogen Sulfide Delivery Molecules Protect Against UVA-Induced Photoaging in Human Dermal Fibroblasts, and in Mouse Skin In Vivo. Antioxid Redox Signal 36:1268-1288 (2022). PubMed: 34235951
- Chen Z et al. Early Application of Quaternized Chitin Derivatives Inhibits Hypertrophic Scar Formation. Macromol Biosci 22:e2100418 (2022). PubMed: 34882969
- Kwon HK et al. Treating 'Septic' With Enhanced Antibiotics and 'Arthritis' by Mitigation of Excessive Inflammation. Front Cell Infect Microbiol 12:897291 (2022). PubMed: 35755835
- Zhong L et al. Glutamine exerts a protective effect on osteoarthritis development by inhibiting the Jun N-terminal kinase and nuclear factor kappa-B signaling pathways. Sci Rep 12:11957 (2022). PubMed: 35831464