Anti-MMP1 antibody (ab2978)
- Datasheet
- References
- Protocols
Overview
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Product nameAnti-MMP1 antibody
See all MMP1 primary antibodies -
DescriptionRabbit polyclonal to MMP1
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Host speciesRabbit
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Tested applicationsSuitable for: IHC-Pmore details
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Species reactivityReacts with: Human
Predicted to work with: Rabbit
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Immunogen
Synthetic peptide within Human MMP1 aa 350-450 (C terminal). The exact sequence is proprietary.
Database link: P03956 -
Positive control
- IHC-P: Human placenta tissue.
Properties
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FormLiquid
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Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
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Storage bufferpH: 7.6
Preservative: 0.1% Sodium azide
Constituents: PBS, 1% BSA -
Concentration information loading... -
PurityImmunogen affinity purified
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ClonalityPolyclonal
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IsotypeIgG
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Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Related Products
Applications
Our Abpromise guarantee covers the use of ab2978 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| IHC-P | 1/200. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. Primary antibody incubation for 10 minutes at RT. |
Target
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FunctionCleaves collagens of types I, II, and III at one site in the helical domain. Also cleaves collagens of types VII and X. In case of HIV infection, interacts and cleaves the secreted viral Tat protein, leading to a decrease in neuronal Tat's mediated neurotoxicity.
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Sequence similaritiesBelongs to the peptidase M10A family.
Contains 4 hemopexin-like domains. -
DomainThere are two distinct domains in this protein; the catalytic N-terminal, and the C-terminal which is involved in substrate specificity and in binding TIMP (tissue inhibitor of metalloproteinases).
The conserved cysteine present in the cysteine-switch motif binds the catalytic zinc ion, thus inhibiting the enzyme. The dissociation of the cysteine from the zinc ion upon the activation-peptide release activates the enzyme. -
Post-translational
modificationsUndergoes autolytic cleavage to two major forms (22 kDa and 27 kDa). A minor form (25 kDa) is the glycosylated form of the 22 kDa form. The 27 kDa form has no activity while the 22/25 kDa form can act as activator for collagenase. -
Cellular localizationSecreted > extracellular space > extracellular matrix.
- Information by UniProt
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Database links
- Entrez Gene: 4312 Human
- Entrez Gene: 100009110 Rabbit
- Omim: 120353 Human
- SwissProt: P03956 Human
- SwissProt: P13943 Rabbit
- Unigene: 83169 Human
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Alternative names
- 27 kDa interstitial collagenase antibody
- CLG antibody
- CLGN antibody
see all
Images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MMP1 antibody (ab2978)Formalin-fixed, paraffin-embedded human placenta tissue stained for MMP1 using ab2978 at 1/200 dilution in immunohistochemical analysis.
Datasheets and documents
References
ab2978 has not yet been referenced specifically in any publications.
