Product nameAnti-MMP1 antibody
See all MMP1 primary antibodies
DescriptionRabbit polyclonal to MMP1
SpecificityThis antibody binds to MMP1, but does not cross react with the other MMP family members (MMP2, MMP3, MMP9).
Tested applicationsSuitable for: IHC-P, ICC/IF, IHC-Fr, WBmore details
Species reactivityReacts with: Horse, Dog, Human
Synthetic peptide corresponding to MMP1. based on the hemopexin domain of the human sequence
(Peptide available as
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferPreservative: 0.05% Sodium azide
Constituent: 50% Glycerol
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab38929 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||Use at an assay dependent concentration.|
|ICC/IF||Use at an assay dependent concentration. PubMed: 18434409|
|IHC-Fr||Use at an assay dependent concentration.|
|WB||1/5000. Predicted molecular weight: 54 kDa. When used against the reduced protein this antibody identifies bands at 53 Kd and 51 Kd (the pro-form), as well as the initial active forms. Dilution optimised using Chromogenic detection.|
FunctionCleaves collagens of types I, II, and III at one site in the helical domain. Also cleaves collagens of types VII and X. In case of HIV infection, interacts and cleaves the secreted viral Tat protein, leading to a decrease in neuronal Tat's mediated neurotoxicity.
Sequence similaritiesBelongs to the peptidase M10A family.
Contains 4 hemopexin-like domains.
DomainThere are two distinct domains in this protein; the catalytic N-terminal, and the C-terminal which is involved in substrate specificity and in binding TIMP (tissue inhibitor of metalloproteinases).
The conserved cysteine present in the cysteine-switch motif binds the catalytic zinc ion, thus inhibiting the enzyme. The dissociation of the cysteine from the zinc ion upon the activation-peptide release activates the enzyme.
modificationsUndergoes autolytic cleavage to two major forms (22 kDa and 27 kDa). A minor form (25 kDa) is the glycosylated form of the 22 kDa form. The 27 kDa form has no activity while the 22/25 kDa form can act as activator for collagenase.
Cellular localizationSecreted > extracellular space > extracellular matrix.
- Information by UniProt
- 27 kDa interstitial collagenase antibody
- CLG antibody
- CLGN antibody
All lanes : Anti-MMP1 antibody (ab38929)
Lane 1 : Human MMP1.
Lane 2 : Cell Media from human chondrosarcoma (no treatment).
Lane 3 : Cell Media from human chondrosarcoma (treated with TPA).
Predicted band size: 54 kDa
Observed band size: 53 kDa why is the actual band size different from the predicted?
ab47116 (2µg/ml) staining Zinc Alpha 2 Glycoprotein in human breast tumour using an automated system (DAKO Autostainer Plus). Using this protocol there is strong staining in Myoepithelial cells.
Sections were rehydrated and antigen retrieved with the Dako 3 in 1 AR buffers citrate pH6.1 in a DAKO PT link. Slides were peroxidase blocked in 3% H2O2 in methanol for 10 mins. They were then blocked with Dako Protein block for 10 minutes (containing casein 0.25% in PBS) then incubated with primary antibody for 20 min and detected with Dako envision flex amplification kit for 30 minutes. Colorimetric detection was completed with Diaminobenzidine for 5 minutes. Slides were counterstained with Haematoxylin and coverslipped under DePeX. Please note that, for manual staining, optimization of primary antibody concentration and incubation time is recommended. Signal amplification may be required.
ab38929 staining MMP1 in Horse fibroblast cells by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with formaldehyde and blocked with 0.1% serum for 30 minutes. Samples were incubated with primary antibody (1/100) for 1 hour. An undiluted FITC-conjugated Mouse anti-rabbit IgG polyclonal was used as the secondary antibody. F-actin stained red and nuclear stained in blue with DAPI
This product has been referenced in:
- Chen H et al. Inhibitory Effects of Plumbagin on Retinal Pigment Epithelial Cell Epithelial-Mesenchymal Transition In Vitro and In Vivo. Med Sci Monit 24:1502-1510 (2018). Read more (PubMed: 29532788) »
- Liu MM et al. Development and Evaluation of a Tissue-Engineered Fibrin-based Canine Mitral Valve Three-dimensional Cell Culture System. J Comp Pathol 160:23-33 (2018). WB, IHC-P ; Dog . Read more (PubMed: 29729718) »