Recombinant
RabMAb

Recombinant Anti-MMP1 antibody [EP1247Y] - Low endotoxin, Azide free (ab215979)

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Overview

  • Product name

    Anti-MMP1 antibody [EP1247Y] - Low endotoxin, Azide free
    See all MMP1 primary antibodies

  • Description

    Rabbit monoclonal [EP1247Y] to MMP1 - Low endotoxin, Azide free

  • Host species

    Rabbit

  • Tested applications

    Suitable for: IHC-P, IHC-Fr, ICC/IF, Flow Cytmore details
    Unsuitable for: IP or WB

  • Species reactivity

    Reacts with: Human

  • Immunogen

    Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) corresponding to Human MMP1 aa 100-200.

  • Positive control

    • IHC-P: Human testis and cervical carcinoma tissues. ICC/IF: HeLa and MCF7 cells. Flow Cyt: HeLa cells.

  • General notes

    ab215979 is a carrier-free antibody designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our Low endotoxin, azide-free formats have low endotoxin level (≤ 1 EU/ml, determined by the LAL assay) and are free from azide, to achieve consistent experimental results in functional assays.

    Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Applications

Our Abpromise guarantee covers the use of ab215979 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P Use at an assay dependent concentration. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

See IHC antigen retrieval protocols.
IHC-Fr Use at an assay dependent concentration. PubMed: 20220088
ICC/IF Use at an assay dependent concentration.
Flow Cyt Use at an assay dependent concentration.

ab199376 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
  • Application notes
    Is unsuitable for IP or WB.

    • Target

    • Function

      Cleaves collagens of types I, II, and III at one site in the helical domain. Also cleaves collagens of types VII and X. In case of HIV infection, interacts and cleaves the secreted viral Tat protein, leading to a decrease in neuronal Tat's mediated neurotoxicity.

    • Sequence similarities

      Belongs to the peptidase M10A family.
      Contains 4 hemopexin-like domains.

    • Domain

      There are two distinct domains in this protein; the catalytic N-terminal, and the C-terminal which is involved in substrate specificity and in binding TIMP (tissue inhibitor of metalloproteinases).
      The conserved cysteine present in the cysteine-switch motif binds the catalytic zinc ion, thus inhibiting the enzyme. The dissociation of the cysteine from the zinc ion upon the activation-peptide release activates the enzyme.

    • Post-translational
      modifications

      Undergoes autolytic cleavage to two major forms (22 kDa and 27 kDa). A minor form (25 kDa) is the glycosylated form of the 22 kDa form. The 27 kDa form has no activity while the 22/25 kDa form can act as activator for collagenase.

    • Cellular localization

      Secreted > extracellular space > extracellular matrix.
    • Information by UniProt

    • Database links

      • Alternative names

        • 27 kDa interstitial collagenase antibody
        • CLG antibody
        • CLGN antibody
        • collagenase, fibroblast antibody
        • collagenase, interstitial antibody
        • Fibroblast collagenase antibody
        • Interstitial collagenase antibody
        • Matrix metallopeptidase 1 (interstitial collagenase) antibody
        • Matrix metalloprotease 1 antibody
        • Matrix Metalloproteinase 1 antibody
        • Matrix metalloproteinase-1 antibody
        • MMP 1 antibody
        • MMP-1 antibody
        • MMP1 antibody
        • MMP1_HUMAN antibody
        • OTTHUMP00000045866 antibody
        see all

      Images

      • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MMP1 antibody [EP1247Y] - Low endotoxin, Azide free (ab215979)
        Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MMP1 antibody [EP1247Y] - Low endotoxin, Azide free (ab215979)This image is courtesy of an anonymous Abreview.

        Formaldehyde-fixed, paraffin-embedded human placenta tissue stained for MMP1 using ab52631 at 1/40 dilution in immunohistochemical analysis.

        This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab52631).

      • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MMP1 antibody [EP1247Y] - Low endotoxin, Azide free (ab215979)
        Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MMP1 antibody [EP1247Y] - Low endotoxin, Azide free (ab215979)

        Immunohistochemical analysis of paraffin-embedded human squamous cell carcinoma of cervix tissue labeling MMP1 with ab52631 at 1/100 dilution followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051, 1/500). Counterstained with hematoxylin.

        This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab52631).

      • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MMP1 antibody [EP1247Y] - Low endotoxin, Azide free (ab215979)
        Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MMP1 antibody [EP1247Y] - Low endotoxin, Azide free (ab215979)

        Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human testis tissue labelling MMP1 with unpurified ab52631 at 1/60. Heat mediated antigen retrieval was performed using Tris/EDTA buffer pH 9. A prediluted HRP-polymer conjugated anti-rabbit IgG was used as the secondary antibody. Negative control using PBS instead of primary antibody. Counterstained with hematoxylin.

        IHC result showed parenchymal cells (such as spermatogonium and spermatocytes) in seminiferous tubules were negative, and the stromal cells were stained.

        This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab52631).

      • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MMP1 antibody [EP1247Y] - Low endotoxin, Azide free (ab215979)
        Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MMP1 antibody [EP1247Y] - Low endotoxin, Azide free (ab215979)

        Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human testis tissue labelling MMP1 with purified ab52631 at 1/100. Heat mediated antigen retrieval was performed using Tris/EDTA buffer pH 9. A prediluted HRP-polymer conjugated anti-rabbit IgG was used as the secondary antibody. Negative control using PBS instead of primary antibody. Counterstained with hematoxylin.

        IHC result showed parenchymal cells (such as spermatogonium and spermatocytes) in seminiferous tubules were negative, and the stromal cells were stained.

        This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab52631).

      • Immunocytochemistry/ Immunofluorescence - Anti-MMP1 antibody [EP1247Y] - Low endotoxin, Azide free (ab215979)
        Immunocytochemistry/ Immunofluorescence - Anti-MMP1 antibody [EP1247Y] - Low endotoxin, Azide free (ab215979)

        Immunocytochemistry/Immunofluorescence analysis of HeLa cells labelling MMP1 with unpurified ab52631 at 1/30. Cells were fixed with 4% paraformaldehyde. An Alexa Fluor® 555-conjugated goat anti-rabbit IgG (1/500) was used as the secondary antibody. DAPI (blue) was used as the nuclear counterstain.

        Control: primary antibody (1/30) and secondary antibody, ab150120, an Alexa Fluor® 594-conjugated goat anti-mouse IgG (1/500).

        This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab52631).

      • Immunocytochemistry/ Immunofluorescence - Anti-MMP1 antibody [EP1247Y] - Low endotoxin, Azide free (ab215979)
        Immunocytochemistry/ Immunofluorescence - Anti-MMP1 antibody [EP1247Y] - Low endotoxin, Azide free (ab215979)

        Immunocytochemistry/Immunofluorescence analysis of HeLa cells labelling MMP1 with purified ab52631 at 1/50. Cells were fixed with 4% paraformaldehyde. An Alexa Fluor® 555-conjugated goat anti-rabbit IgG (1/500) was used as the secondary antibody. DAPI (blue) was used as the nuclear counterstain.

        Control: primary antibody (1/50) and secondary antibody, ab150120, an Alexa Fluor® 594-conjugated goat anti-mouse IgG (1/500).

        This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab52631).

      • Flow Cytometry - Anti-MMP1 antibody [EP1247Y] - Low endotoxin, Azide free (ab215979)
        Flow Cytometry - Anti-MMP1 antibody [EP1247Y] - Low endotoxin, Azide free (ab215979)

        Flow cytometry analysis of HeLa cells labelling MMP1 with unpurified ab52631 at 1/50 (red). Cells were fixed with 2% paraformaldehyde. A FITC-conjugated goat anti-rabbit IgG (1/150) was used as the secondary antibody. Green - Isotype control, rabbit monoclonal IgG.

        This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab52631).

      • Flow Cytometry - Anti-MMP1 antibody [EP1247Y] - Low endotoxin, Azide free (ab215979)
        Flow Cytometry - Anti-MMP1 antibody [EP1247Y] - Low endotoxin, Azide free (ab215979)

        Flow cytometry analysis of HeLa cells labelling MMP1 with purified ab52631 at 1/70 (red). Cells were fixed with 2% paraformaldehyde. A FITC-conjugated goat anti-rabbit IgG was used as the secondary antibody (1/150). Green - Isotype control, rabbit monoclonal IgG.

        This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab52631).

      • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MMP1 antibody [EP1247Y] - Low endotoxin, Azide free (ab215979)
        Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MMP1 antibody [EP1247Y] - Low endotoxin, Azide free (ab215979)

        Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human cervical carcinoma tissue labelling MMP1 with ab52631 at 1/50.

        This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab52631).

      • Immunocytochemistry/ Immunofluorescence - Anti-MMP1 antibody [EP1247Y] - Low endotoxin, Azide free (ab215979)
        Immunocytochemistry/ Immunofluorescence - Anti-MMP1 antibody [EP1247Y] - Low endotoxin, Azide free (ab215979)

        ICC/IF image of unpurified ab52631 stained MCF7 cells. The cells were 4% PFA fixed (10 min) and then incubated in 1% BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab52631, 1/1000 dilution) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

        This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab52631).

      References

      This product has been referenced in:

      • Norsgaard H  et al. Calcipotriol counteracts betamethasone-induced decrease in extracellular matrix components related to skin atrophy. Arch Dermatol Res 306:719-29 (2014). Human . Read more (PubMed: 25027750) »
      • Tewari A  et al. Upregulation of MMP12 and its activity by UVA1 in human skin: potential implications for photoaging. J Invest Dermatol 134:2598-609 (2014). IF ; Human . Read more (PubMed: 24714202) »
      See all 11 Publications for this product

      Publishing research using ab215979? Please let us know so that we can cite the reference in this datasheet.

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