Overview

  • Product name

    Anti-MMP1 antibody [EP1249Y]
    See all MMP1 primary antibodies
  • Description

    Rabbit monoclonal [EP1249Y] to MMP1
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IPmore details
    Unsuitable for: Flow Cyt,ICC or IHC-P
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Synthetic peptide within Human MMP1 aa 1-100 (N terminal). The exact sequence is proprietary. The immunogen amino acid sequence is within the propeptide sequence.

  • Positive control

    • A431 cell lysate
  • General notes

    A trial size is available to purchase for this antibody.

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab134184 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000. Predicted molecular weight: 54 kDa.
IP 1/20.
  • Application notes
    Is unsuitable for Flow Cyt,ICC or IHC-P.
  • Target

    • Function

      Cleaves collagens of types I, II, and III at one site in the helical domain. Also cleaves collagens of types VII and X. In case of HIV infection, interacts and cleaves the secreted viral Tat protein, leading to a decrease in neuronal Tat's mediated neurotoxicity.
    • Sequence similarities

      Belongs to the peptidase M10A family.
      Contains 4 hemopexin-like domains.
    • Domain

      There are two distinct domains in this protein; the catalytic N-terminal, and the C-terminal which is involved in substrate specificity and in binding TIMP (tissue inhibitor of metalloproteinases).
      The conserved cysteine present in the cysteine-switch motif binds the catalytic zinc ion, thus inhibiting the enzyme. The dissociation of the cysteine from the zinc ion upon the activation-peptide release activates the enzyme.
    • Post-translational
      modifications

      Undergoes autolytic cleavage to two major forms (22 kDa and 27 kDa). A minor form (25 kDa) is the glycosylated form of the 22 kDa form. The 27 kDa form has no activity while the 22/25 kDa form can act as activator for collagenase.
    • Cellular localization

      Secreted > extracellular space > extracellular matrix.
    • Information by UniProt
    • Database links

    • Alternative names

      • 27 kDa interstitial collagenase antibody
      • CLG antibody
      • CLGN antibody
      • collagenase, fibroblast antibody
      • collagenase, interstitial antibody
      • Fibroblast collagenase antibody
      • Interstitial collagenase antibody
      • Matrix metallopeptidase 1 (interstitial collagenase) antibody
      • Matrix metalloprotease 1 antibody
      • Matrix Metalloproteinase 1 antibody
      • Matrix metalloproteinase-1 antibody
      • MMP 1 antibody
      • MMP-1 antibody
      • MMP1 antibody
      • MMP1_HUMAN antibody
      • OTTHUMP00000045866 antibody
      see all

    Images

    • All lanes : Anti-MMP1 antibody [EP1249Y] (ab134184) at 1/500 dilution

      Lane 1 : MDA-MB-231 (human breast adenocarcinoma epithelial) whole cell lysate
      Lane 2 : Lysate from HeLa cells untreated with TPA
      Lane 3 : HeLa treated with 100 ng/mL TPA for 10 hours cell lysate
      Lane 4 : Human colon cancer
      Lane 5 : Human breast cancer

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : Goat Anti-Rabbit IgG H&L (HRP) at 1/1000 dilution

      Predicted band size: 54 kDa
      Observed band size: 54 kDa


      Exposure time: 30 seconds


      Blocking and dilution buffer: 5% NFDM/TBST

    • Anti-MMP1 antibody [EP1249Y] (ab134184) at 1/1000 dilution + A431 cell lysate at 10 µg

      Secondary
      HRP labelled goat anti-rabbit at 1/2000 dilution

      Predicted band size: 54 kDa

    References

    This product has been referenced in:

    • Seidl CI  et al. CRISPR-Cas9 targeting of MMP13 in human chondrocytes leads to significantly reduced levels of the metalloproteinase and enhanced type II collagen accumulation. Osteoarthritis Cartilage 27:140-147 (2019). Read more (PubMed: 30223022) »
    • Waasdorp M  et al. Protease-activated receptor-1 contributes to renal injury and interstitial fibrosis during chronic obstructive nephropathy. J Cell Mol Med 23:1268-1279 (2019). Read more (PubMed: 30485646) »
    See all 3 Publications for this product

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