Overview

  • Product name

    Anti-MMP1 antibody [EPR19769-2]
    See all MMP1 primary antibodies
  • Description

    Rabbit monoclonal [EPR19769-2] to MMP1
  • Host species

    Rabbit
  • Tested applications

    Suitable for: Sandwich ELISAmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    The details of the immunogen for this antibody are not available.

  • General notes

    Anti-MMP1 antibody is used in our 90-minute Human MMP1 ELISA Kit (ab215083), and is available in a Matched Antibody Pair Kit (ab218184).

Properties

Applications

Our Abpromise guarantee covers the use of ab222582 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Sandwich ELISA Use at an assay dependent concentration.

Target

  • Function

    Cleaves collagens of types I, II, and III at one site in the helical domain. Also cleaves collagens of types VII and X. In case of HIV infection, interacts and cleaves the secreted viral Tat protein, leading to a decrease in neuronal Tat's mediated neurotoxicity.
  • Sequence similarities

    Belongs to the peptidase M10A family.
    Contains 4 hemopexin-like domains.
  • Domain

    There are two distinct domains in this protein; the catalytic N-terminal, and the C-terminal which is involved in substrate specificity and in binding TIMP (tissue inhibitor of metalloproteinases).
    The conserved cysteine present in the cysteine-switch motif binds the catalytic zinc ion, thus inhibiting the enzyme. The dissociation of the cysteine from the zinc ion upon the activation-peptide release activates the enzyme.
  • Post-translational
    modifications

    Undergoes autolytic cleavage to two major forms (22 kDa and 27 kDa). A minor form (25 kDa) is the glycosylated form of the 22 kDa form. The 27 kDa form has no activity while the 22/25 kDa form can act as activator for collagenase.
  • Cellular localization

    Secreted > extracellular space > extracellular matrix.
  • Information by UniProt
  • Database links

  • Alternative names

    • 27 kDa interstitial collagenase antibody
    • CLG antibody
    • CLGN antibody
    • collagenase, fibroblast antibody
    • collagenase, interstitial antibody
    • Fibroblast collagenase antibody
    • Interstitial collagenase antibody
    • Matrix metallopeptidase 1 (interstitial collagenase) antibody
    • Matrix metalloprotease 1 antibody
    • Matrix Metalloproteinase 1 antibody
    • Matrix metalloproteinase-1 antibody
    • MMP 1 antibody
    • MMP-1 antibody
    • MMP1 antibody
    • MMP1_HUMAN antibody
    • OTTHUMP00000045866 antibody
    see all

Images

  • Example of human MMP1 standard curve in 1X Cell Extraction Buffer PTR using recombinant MMP1 RabMAb antibody (ab222582) and a recombinant monoclonal antibody. Background-subtracted values (mean +/- SD) are graphed.

    For a complete ELISA kit, see Human MMP1 ELISA kit (ab215083).

    For a matched antibody pair set, see Human MMP1 Matched Antibody Pair Kit (ab218184).

     

References

ab222582 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

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