Overview

  • Product name

  • Description

    Rabbit polyclonal to MMP10
  • Host species

    Rabbit
  • Tested applications

    Suitable for: ICC/IF, IHC-P, WBmore details
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide within Human MMP10 aa 380-410. The exact sequence is proprietary.
    Database link: P09238

  • Positive control

    • A549 cell lysate; HepG2 cells; human lung carcinoma tissue.

Properties

Applications

Our Abpromise guarantee covers the use of ab199688 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF 1/50 - 1/100.
IHC-P 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
WB 1/1000. Predicted molecular weight: 54 kDa.

Target

  • Function

    Can degrade fibronectin, gelatins of type I, III, IV, and V; weakly collagens III, IV, and V. Activates procollagenase.
  • Sequence similarities

    Belongs to the peptidase M10A family.
    Contains 4 hemopexin-like domains.
  • Domain

    The conserved cysteine present in the cysteine-switch motif binds the catalytic zinc ion, thus inhibiting the enzyme. The dissociation of the cysteine from the zinc ion upon the activation-peptide release activates the enzyme.
  • Cellular localization

    Secreted > extracellular space > extracellular matrix.
  • Information by UniProt
  • Database links

  • Alternative names

    • Matrix metallopeptidase 10 (stromelysin 2) antibody
    • Matrix metalloprotease 10 antibody
    • Matrix metalloproteinase 10 (stromelysin 2) antibody
    • Matrix metalloproteinase 10 antibody
    • Matrix metalloproteinase-10 antibody
    • MMP 10 antibody
    • MMP-10 antibody
    • Mmp10 antibody
    • MMP10_HUMAN antibody
    • SL 2 antibody
    • SL-2 antibody
    • SL2 antibody
    • STMY 2 antibody
    • STMY2 antibody
    • Stromelysin 2 antibody
    • Stromelysin II antibody
    • Stromelysin-2 antibody
    • Stromelysin2 antibody
    • StromelysinII antibody
    • Transin 2 antibody
    • Transin-2 antibody
    • Transin2 antibody
    see all

Images

  • Anti-MMP10 antibody (ab199688) at 1/1000 dilution + A549 cell lysate at 35 µg

    Secondary
    Goat anti-Rabbit IgG H&L (HRP) at 1/5000 dilution

    Developed using the ECL technique.

    Predicted band size: 54 kDa

  • Immunohistochemical analysis of paraffin embedded human lung carcinoma tissue labeling MMP10 with ab199688 at 1/50 dilution.

  • Immunofluorescence analysis of HepG2 cells labeling MMP10 with ab199688 at 1/100 dilution.

References

This product has been referenced in:

  • Liu X  et al. Heparanase Promotes Tumor Growth and Liver Metastasis of Colorectal Cancer Cells by Activating the p38/MMP1 Axis. Front Oncol 9:216 (2019). Read more (PubMed: 31001480) »
  • Liang Y  et al. LncRNA CASC9 promotes esophageal squamous cell carcinoma metastasis through upregulating LAMC2 expression by interacting with the CREB-binding protein. Cell Death Differ 25:1980-1995 (2018). Read more (PubMed: 29511340) »
See all 3 Publications for this product

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab199688.
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Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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