• Product name

  • Description

    Rabbit polyclonal to MMP10
  • Host species

  • Specificity

    The antibody binds to the hinge region of MMP10. The antibody binds to Stromelysin 2, but does not cross react with the other MMP family members (MMP1, MMP2, MMP3, etc.). The antibody also recognizes the cleaved active enzyme and will also bind to the non-reduced protein.
  • Tested applications

    Suitable for: IHC-P, WB, IHC-Frmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Synthetic peptide based on the hinge region of the human MMP10 sequence.

    Read Abcam's proprietary immunogen policy (Peptide available as ab41075.)



Our Abpromise guarantee covers the use of ab38930 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
  • Application notes
    IHC-P: Use at a concentration of 2 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
    WB: Use at an assay dependent dilution. Predicted molecular weight: 54 kDa. A recommended starting concentration for Western blots is 1/1000 when using colorimetric substrates such as BCIP/NBT, and 1/5000 for chemiluminescent substrates. Although the sequence homology for this portion of MMP10 is well conserved, higher concentrations of antibody may be needed for non-human samples. When used against the reduced protein the antibody identifies bands at 56 kDa (the pro-form), as well as the initial active forms (46 kDa). Dilution optimised using Chromogenic detection. Not yet tested in other applications. Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • Function

      Can degrade fibronectin, gelatins of type I, III, IV, and V; weakly collagens III, IV, and V. Activates procollagenase.
    • Sequence similarities

      Belongs to the peptidase M10A family.
      Contains 4 hemopexin-like domains.
    • Domain

      The conserved cysteine present in the cysteine-switch motif binds the catalytic zinc ion, thus inhibiting the enzyme. The dissociation of the cysteine from the zinc ion upon the activation-peptide release activates the enzyme.
    • Cellular localization

      Secreted > extracellular space > extracellular matrix.
    • Information by UniProt
    • Database links

    • Alternative names

      • Matrix metallopeptidase 10 (stromelysin 2) antibody
      • Matrix metalloprotease 10 antibody
      • Matrix metalloproteinase 10 (stromelysin 2) antibody
      • Matrix metalloproteinase 10 antibody
      • Matrix metalloproteinase-10 antibody
      • MMP 10 antibody
      • MMP-10 antibody
      • Mmp10 antibody
      • MMP10_HUMAN antibody
      • SL 2 antibody
      • SL-2 antibody
      • SL2 antibody
      • STMY 2 antibody
      • STMY2 antibody
      • Stromelysin 2 antibody
      • Stromelysin II antibody
      • Stromelysin-2 antibody
      • Stromelysin2 antibody
      • StromelysinII antibody
      • Transin 2 antibody
      • Transin-2 antibody
      • Transin2 antibody
      see all


    • Ab38930 staining Human normal trachea. Staining is localised to the cytoplasm.
      Left panel: with primary antibody at 2 ug/ml. Right panel: isotype control.
      Sections were stained using an automated system DAKO Autostainer Plus , at room temperature: sections were rehydrated and antigen retrieved with the Dako 3 in 1 AR buffers citrate pH 6.0 in a DAKO PT Link. Slides were peroxidase blocked in 3% H2O2 in methanol for 10 mins. They were then blocked with Dako Protein block for 10 minutes (containing casein 0.25% in PBS) then incubated with primary antibody for 20 min and detected with Dako envision flex amplification kit for 30 minutes. Colorimetric detection was completed with Diaminobenzidine for 5 minutes. Slides were counterstained with Haematoxylin and coverslipped under DePeX. Please note that for manual staining we recommend to optimize the primary antibody concentration and incubation time (overnight incubation), and amplification may be required.


    This product has been referenced in:

    • Brilha S  et al. Early Secretory Antigenic Target-6 Drives Matrix Metalloproteinase-10 Gene Expression and Secretion in Tuberculosis. Am J Respir Cell Mol Biol 56:223-232 (2017). WB ; Human . Read more (PubMed: 27654284) »
    • Ehlken C  et al. Increased expression of angiogenic and inflammatory proteins in the vitreous of patients with ischemic central retinal vein occlusion. PLoS One 10:e0126859 (2015). IHC . Read more (PubMed: 25978399) »
    See all 4 Publications for this product

    Customer reviews and Q&As

    1-2 of 2 Abreviews or Q&A

    Western blot
    Human Cell lysate - whole cell (endothelial cells)
    Loading amount
    5000 cells
    endothelial cells
    Gel Running Conditions
    Reduced Denaturing (10% acrylamide gel)
    Blocking step
    BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 21°C

    Abcam user community

    Verified customer

    Submitted Mar 01 2013

    Immunohistochemistry (Frozen sections)
    Human Tissue lysate - whole (Human Skin)
    Human Skin
    Blocking step
    Serum as blocking agent for 20 minute(s) · Concentration: 0.02

    Abcam user community

    Verified customer

    Submitted Aug 26 2005

    For licensing inquiries, please contact partnerships@abcam.com

    Sign up