Key features and details
- Rabbit polyclonal to MMP10
- Suitable for: IHC-P, WB, IHC-Fr
- Reacts with: Human
- Isotype: IgG
Product nameAnti-MMP10 antibody
See all MMP10 primary antibodies
DescriptionRabbit polyclonal to MMP10
SpecificityThe antibody binds to the hinge region of MMP10. The antibody binds to Stromelysin 2, but does not cross react with the other MMP family members (MMP1, MMP2, MMP3, etc.). The antibody also recognizes the cleaved active enzyme and will also bind to the non-reduced protein.
Tested applicationsSuitable for: IHC-P, WB, IHC-Frmore details
Species reactivityReacts with: Human
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage bufferPreservative: 0.05% Sodium azide
Constituent: 50% Glycerol
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab38930 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
WB: Use at an assay dependent dilution. Predicted molecular weight: 54 kDa. A recommended starting concentration for Western blots is 1/1000 when using colorimetric substrates such as BCIP/NBT, and 1/5000 for chemiluminescent substrates. Although the sequence homology for this portion of MMP10 is well conserved, higher concentrations of antibody may be needed for non-human samples. When used against the reduced protein the antibody identifies bands at 56 kDa (the pro-form), as well as the initial active forms (46 kDa). Dilution optimised using Chromogenic detection. Not yet tested in other applications. Optimal dilutions/concentrations should be determined by the end user.
FunctionCan degrade fibronectin, gelatins of type I, III, IV, and V; weakly collagens III, IV, and V. Activates procollagenase.
Sequence similaritiesBelongs to the peptidase M10A family.
Contains 4 hemopexin-like domains.
DomainThe conserved cysteine present in the cysteine-switch motif binds the catalytic zinc ion, thus inhibiting the enzyme. The dissociation of the cysteine from the zinc ion upon the activation-peptide release activates the enzyme.
Cellular localizationSecreted > extracellular space > extracellular matrix.
- Information by UniProt
- Matrix metallopeptidase 10 (stromelysin 2) antibody
- Matrix metalloprotease 10 antibody
- Matrix metalloproteinase 10 (stromelysin 2) antibody
Ab38930 staining Human normal trachea. Staining is localised to the cytoplasm.
Left panel: with primary antibody at 2 ug/ml. Right panel: isotype control.
Sections were stained using an automated system DAKO Autostainer Plus , at room temperature: sections were rehydrated and antigen retrieved with the Dako 3 in 1 AR buffers citrate pH 6.0 in a DAKO PT Link. Slides were peroxidase blocked in 3% H2O2 in methanol for 10 mins. They were then blocked with Dako Protein block for 10 minutes (containing casein 0.25% in PBS) then incubated with primary antibody for 20 min and detected with Dako envision flex amplification kit for 30 minutes. Colorimetric detection was completed with Diaminobenzidine for 5 minutes. Slides were counterstained with Haematoxylin and coverslipped under DePeX. Please note that for manual staining we recommend to optimize the primary antibody concentration and incubation time (overnight incubation), and amplification may be required.
ab38930 has been referenced in 4 publications.
- Brilha S et al. Early Secretory Antigenic Target-6 Drives Matrix Metalloproteinase-10 Gene Expression and Secretion in Tuberculosis. Am J Respir Cell Mol Biol 56:223-232 (2017). WB ; Human . PubMed: 27654284
- Ehlken C et al. Increased expression of angiogenic and inflammatory proteins in the vitreous of patients with ischemic central retinal vein occlusion. PLoS One 10:e0126859 (2015). IHC . PubMed: 25978399
- Zhang G et al. Validation and clinicopathologic associations of a urine-based bladder cancer biomarker signature. Diagn Pathol 9:200 (2014). IHC ; Human . PubMed: 25387487
- Frederick LA et al. Matrix metalloproteinase-10 is a critical effector of protein kinase Ciota-Par6alpha-mediated lung cancer. Oncogene 27:4841-53 (2008). IHC-P . PubMed: 18427549