Overview

  • Product name

  • Description

    Rabbit polyclonal to MMP10
  • Host species

    Rabbit
  • Specificity

    Detects endogenous levels of total MMP10 protein.
  • Tested applications

    Suitable for: ICC/IF, ELISA, Flow Cyt, IHC-Frmore details
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide (Human) from an internal region of MMP10.

  • Positive control

    • HepG2 cells.

Properties

Applications

Our Abpromise guarantee covers the use of ab59437 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF 1/500 - 1/1000.
ELISA 1/10000.
Flow Cyt Use at an assay dependent concentration. PubMed: 21133852

ab171870 - Rabbit polyclonal IgG, is suitable for use as an isotype control with this antibody.

 

IHC-Fr Use at an assay dependent concentration. PubMed: 21133852

Target

  • Function

    Can degrade fibronectin, gelatins of type I, III, IV, and V; weakly collagens III, IV, and V. Activates procollagenase.
  • Sequence similarities

    Belongs to the peptidase M10A family.
    Contains 4 hemopexin-like domains.
  • Domain

    The conserved cysteine present in the cysteine-switch motif binds the catalytic zinc ion, thus inhibiting the enzyme. The dissociation of the cysteine from the zinc ion upon the activation-peptide release activates the enzyme.
  • Cellular localization

    Secreted > extracellular space > extracellular matrix.
  • Information by UniProt
  • Database links

  • Alternative names

    • Matrix metallopeptidase 10 (stromelysin 2) antibody
    • Matrix metalloprotease 10 antibody
    • Matrix metalloproteinase 10 (stromelysin 2) antibody
    • Matrix metalloproteinase 10 antibody
    • Matrix metalloproteinase-10 antibody
    • MMP 10 antibody
    • MMP-10 antibody
    • Mmp10 antibody
    • MMP10_HUMAN antibody
    • SL 2 antibody
    • SL-2 antibody
    • SL2 antibody
    • STMY 2 antibody
    • STMY2 antibody
    • Stromelysin 2 antibody
    • Stromelysin II antibody
    • Stromelysin-2 antibody
    • Stromelysin2 antibody
    • StromelysinII antibody
    • Transin 2 antibody
    • Transin-2 antibody
    • Transin2 antibody
    see all

Images

  • Immunofluorescent analysis of MMP10 in HepG2 cells with ab59437 (1/500 dilution); in the presence or absence of immunizing peptide.

References

This product has been referenced in:

  • Rowbotham SP  et al. H3K9 methyltransferases and demethylases control lung tumor-propagating cells and lung cancer progression. Nat Commun 9:4559 (2018). Read more (PubMed: 30455465) »
  • Clark RT  et al. T cell production of matrix metalloproteases and inhibition of parasite clearance by TIMP-1 during chronic Toxoplasma infection in the brain. ASN Neuro : (2010). Flow Cyt, IHC-Fr ; Mouse . Read more (PubMed: 21133852) »
See all 2 Publications for this product

Customer reviews and Q&As

1-2 of 2 Abreviews or Q&A

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Human Tissue sections (First trimester decidua)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Sodium citrate
Permeabilization
Yes - 0.5% Triton
Specification
First trimester decidua
Blocking step
BSA as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 22°C
Fixative
Formaldehyde

Ruhul Choudhury

Verified customer

Submitted Jan 25 2017

Answer

Thank you very much for your interest in Abcam and our products..

To our knowledge,the following antibodies you listed have not been tested in IHC-P. Tested applications and species are listed on the individual datasheets. These are updated as soon as we receive any new information.

ab25644
ab24941
ab86900
ab41467,
ab60077,
ab25175,
ab24985,
ab59437,
ab116223

Therefore, I can offer a discount off a future purchase if you buythese untested antibodies now, test in IHC-P and submit feedback to us in the form of an Abreview. It doesn't matter whether the Abreview is positive or negative, we would just really like to receive your feedback.One discount code would be provided for each individualantibody thatwould be to the value of 1 free primary antibody.

If you are interested in this offer, please follow these steps:

1. Reply to this e-mail to let me know that you would like to proceed.If you could let me know whichparticular antibodies youwant to test. I will then send a discount code for each of these antibody. This code must be issued before purchasing the antibodiesso please wait for my reply before ordering.

2. Purchase the antibodies either by phone, fax, or online (www.abcam.com).

3. Test it in IHC-P.

4. Let us know the results, positive or negative, using our Abreview system (this will take about 10 minutes and images are great if you have them!). To find out how to submit an Abreview, please visit: https://www.abcam.com/abreviews.

5. After the review is submitted to us, the discount code becomes active. Simply place your new order by phone, fax, or on the web and mention the discount code. The discount can be redeemed for any primary antibody ordered and the discount code is valid for 4 months after issue.

We are always pleased to obtain feedback about our products and any information is greatly appreciated! Even if the any of the antibodiesturns out to be unsuitable for IHC-P, you will still receive the discount on your next purchase after your Abreview has been submitted.

Please let me know if you have any questions about this offer and I would be happy to help you further.

The Terms and Conditions of this offer can be found at: www.abcam.com/collaborationdiscount.

The following antibodies you listed have already been tested in IHC-P, and so we are not able to provide a testing discount for these on this occasion. As they have been tested in IHC-P, we can guarantee them for fluorescent studies on paraffin embedded tissue as well.

ab112470
ab61219
ab6564

We do not charge individual shipping for each item. We would charge one shipping fee for eachorder. Extra shipping charges are applied if there is a bulky item such as a kit, or a dry ice item on the order.

With regards to discounts for new laboratories, I have spoken to our marketing department and believe thatone ofyour colleagues, Dr. ##### has already been in touch with us. A 25% discount has been arranged for orders from his laboratory for the next 1 month. Could you confirm that you are working in the same laboratories? I hope this discount will prove helpful in setting up the new laboratory. As we have already arranged these 2 types of discount, I am sorry we are not able to offer a further bulk discount or shipping discount on this occasion.

I hope this will be helpful. If you have any further questions, please do not hesitate to contact us.

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