Key features and details
- Assay type: Enzyme activity
- Detection method: Fluorescent
- Platform: Microplate reader
- Sample type: Inhibitor compounds
Product nameMMP10 Inhibitor Screening Assay Kit (Fluorometric)
See all MMP10 kits
Sample typeInhibitor compounds
Assay typeEnzyme activity
Abcam MMP10 Inhibitor Screening Assay Kit (Fluorometric) (ab139457) is a complete assay system designed to screen MMP10 inhibitors using a quenched fluorogenic peptide: MMP Fluorogenic Substrate Mca-Pro-Leu-Gly-Leu-Dpa-Ala-Arg-NH2 [Mca=(7-methoxycoumarin-4-yl)-acetyl; Dpa=N-3-(2,4-dinitrophenyl)-L-α-β-diaminopropionyl]. Mca fluorescence is quenched by the Dpa group until cleavage by MMPs at the Gly-Leu bond separates the two moieties. The assays are performed in a convenient 96-well microplate format.
This kit is useful to screen inhibitors of MMP10, a potential therapeutic target. The MMP inhibitor NNGH is also included as a prototypic control inhibitor.
Storage instructionsPlease refer to protocols.
Components 1 x 96 tests 96-well White Microplate 1/2 Volume 1 unit Fluorometric Assay Buffer 1 x 20ml MMP Calibration Standard 1 x 50µl MMP Fluorogenic Substrate 1 x 200µl MMP Inhibitor 1 x 50µl MMP10 Enzyme (Human, Recombinant) 1 x 30µl
FunctionCan degrade fibronectin, gelatins of type I, III, IV, and V; weakly collagens III, IV, and V. Activates procollagenase.
Sequence similaritiesBelongs to the peptidase M10A family.
Contains 4 hemopexin-like domains.
DomainThe conserved cysteine present in the cysteine-switch motif binds the catalytic zinc ion, thus inhibiting the enzyme. The dissociation of the cysteine from the zinc ion upon the activation-peptide release activates the enzyme.
Cellular localizationSecreted > extracellular space > extracellular matrix.
- Information by UniProt
- Matrix metallopeptidase 10 (stromelysin 2)
- Matrix metalloprotease 10
- Matrix metalloproteinase 10
ab139457 has not yet been referenced specifically in any publications.