Product nameAnti-MMP11 antibody
See all MMP11 primary antibodies
DescriptionRabbit polyclonal to MMP11
Specificityab53143 detects endogenous levels of total MMP11 protein.
Tested applicationsSuitable for: IHC-P, WB, ELISAmore details
Species reactivityReacts with: Human
Predicted to work with: Mouse, Rat
Synthesized peptide derived from human MMP11.
- WB: A549 cell extract
Storage instructionsShipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
Storage bufferpH: 7.40
Preservative: 0.02% Sodium azide
Constituents: 50% Glycerol, 0.87% Sodium chloride, PBS
Without Mg+2 and Ca+2
Concentration information loading...
PurityImmunogen affinity purified
Purification notesThe antibody was affinity purified from rabbit antiserum by affinity chromatography using epitope specific immunogen.
Our Abpromise guarantee covers the use of ab53143 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||Use a concentration of 2 µg/ml.|
|WB||1/500 - 1/1000. Detects a band of approximately 62 kDa (predicted molecular weight: 55 kDa).|
FunctionMay play an important role in the progression of epithelial malignancies.
Tissue specificitySpecifically expressed in stromal cells of breast carcinomas.
Sequence similaritiesBelongs to the peptidase M10A family.
Contains 4 hemopexin-like domains.
DomainThe conserved cysteine present in the cysteine-switch motif binds the catalytic zinc ion, thus inhibiting the enzyme. The dissociation of the cysteine from the zinc ion upon the activation-peptide release activates the enzyme.
modificationsThe precursor is cleaved by a furin endopeptidase.
Cellular localizationSecreted > extracellular space > extracellular matrix.
- Information by UniProt
- Matrix Metalloproteinase 11 antibody
- Matrix metalloproteinase-11 antibody
- MMP-11 antibody
All lanes : Anti-MMP11 antibody (ab53143) at 1/500 dilution
Lane 1 : A549 cell extract with no peptide
Lane 2 : A549 cell extract with peptide
Predicted band size: 55 kDa
Observed band size: 62 kDa why is the actual band size different from the predicted?
Ab53143 staining Human normal placenta. Staining is localised to the extracellular matrix (secreted).
Left panel: with primary antibody at 2 ug/ml. Right panel: isotype control.
Sections were stained using an automated system DAKO Autostainer Plus , at room temperature. Sections were rehydrated and antigen retrieved with the Dako 3-in-1 AR buffers EDTA pH 9.0 in a DAKO PT Link. Slides were peroxidase blocked in 3% H2O2 in methanol for 10 minutes. They were then blocked with Dako Protein block for 10 minutes (containing casein 0.25% in PBS) then incubated with primary antibody for 20 minutes and detected with Dako Envision Flex amplification kit for 30 minutes. Colorimetric detection was completed with diaminobenzidine for 5 minutes. Slides were counterstained with Haematoxylin and coverslipped under DePeX. Please note that for manual staining we recommend to optimize the primary antibody concentration and incubation time (overnight incubation), and amplification may be require
This product has been referenced in:
- Niwa T et al. The dynamics of TGF-ß in dental pulp, odontoblasts and dentin. Sci Rep 8:4450 (2018). WB . Read more (PubMed: 29535349) »
- Jiang R et al. Mixomics analysis of breast cancer: Long non-coding RNA linc01561 acts as ceRNA involved in the progression of breast cancer. Int J Biochem Cell Biol 102:1-9 (2018). WB . Read more (PubMed: 29890225) »