• Product name

  • Description

    Rabbit polyclonal to MMP11
  • Host species

  • Specificity

    ab53143 detects endogenous levels of total MMP11 protein.
  • Tested applications

    Suitable for: IHC-P, WB, ELISAmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse, Rat
  • Immunogen

    Synthesized peptide derived from human MMP11.

  • Positive control

    • WB: A549 cell extract



Our Abpromise guarantee covers the use of ab53143 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P Use a concentration of 2 µg/ml.
WB 1/500 - 1/1000. Detects a band of approximately 62 kDa (predicted molecular weight: 55 kDa).
ELISA 1/10000.


  • Function

    May play an important role in the progression of epithelial malignancies.
  • Tissue specificity

    Specifically expressed in stromal cells of breast carcinomas.
  • Sequence similarities

    Belongs to the peptidase M10A family.
    Contains 4 hemopexin-like domains.
  • Domain

    The conserved cysteine present in the cysteine-switch motif binds the catalytic zinc ion, thus inhibiting the enzyme. The dissociation of the cysteine from the zinc ion upon the activation-peptide release activates the enzyme.
  • Post-translational

    The precursor is cleaved by a furin endopeptidase.
  • Cellular localization

    Secreted > extracellular space > extracellular matrix.
  • Information by UniProt
  • Database links

  • Alternative names

    • Matrix Metalloproteinase 11 antibody
    • Matrix metalloproteinase-11 antibody
    • MMP-11 antibody
    • Mmp11 antibody
    • MMP11_HUMAN antibody
    • SL 3 antibody
    • SL-3 antibody
    • SL3 antibody
    • ST3 antibody
    • STMY3 antibody
    • Stromelysin 3 antibody
    • Stromelysin III antibody
    • Stromelysin-3 antibody
    see all


  • All lanes : Anti-MMP11 antibody (ab53143) at 1/500 dilution

    Lane 1 : A549 cell extract with no peptide
    Lane 2 : A549 cell extract with peptide

    Predicted band size: 55 kDa
    Observed band size: 62 kDa
    why is the actual band size different from the predicted?

  • Ab53143 staining Human normal placenta. Staining is localised to the extracellular matrix (secreted).
    Left panel: with primary antibody at 2 ug/ml. Right panel: isotype control.
    Sections were stained using an automated system DAKO Autostainer Plus , at room temperature. Sections were rehydrated and antigen retrieved with the Dako 3-in-1 AR buffers EDTA pH 9.0 in a DAKO PT Link. Slides were peroxidase blocked in 3% H2O2 in methanol for 10 minutes. They were then blocked with Dako Protein block for 10 minutes (containing casein 0.25% in PBS) then incubated with primary antibody for 20 minutes and detected with Dako Envision Flex amplification kit for 30 minutes. Colorimetric detection was completed with diaminobenzidine for 5 minutes. Slides were counterstained with Haematoxylin and coverslipped under DePeX. Please note that for manual staining we recommend to optimize the primary antibody concentration and incubation time (overnight incubation), and amplification may be require


This product has been referenced in:

  • Niwa T  et al. The dynamics of TGF-ß in dental pulp, odontoblasts and dentin. Sci Rep 8:4450 (2018). WB . Read more (PubMed: 29535349) »
  • Jiang R  et al. Mixomics analysis of breast cancer: Long non-coding RNA linc01561 acts as ceRNA involved in the progression of breast cancer. Int J Biochem Cell Biol 102:1-9 (2018). WB . Read more (PubMed: 29890225) »
See all 3 Publications for this product

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