Anti-MMP11 antibody [OTI3E10] (ab236510)

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Overview

  • Product name

    Anti-MMP11 antibody [OTI3E10]
    See all MMP11 primary antibodies

  • Description

    Mouse monoclonal [OTI3E10] to MMP11

  • Host species

    Mouse

  • Tested applications

    Suitable for: WBmore details

  • Species reactivity

    Reacts with: Human

  • Immunogen

    Recombinant fragment corresponding to Human MMP11 aa 239-488. (NP_005931) produced in E.coli
    Sequence:

    FRYPLSLSPDDCRGVQHLYGQPWPTVTSRTPALGPQAGIDTNEIAPLEPD APPDACEASFDAVSTIRGELFFFKAGFVWRLRGGQLQPGYPALASRHWQG LPSPVDAAFEDAQGHIWFFQGAQYWVYDGEKPVLGPAPLTELGLVRFPVH AALVWGPEKNKIYFFRGRDYWRFHPSTRRVDSPVPRRATDWRGVPSEIDA AFQDADGYAYFLRGRLYWKFDPVKVKALEGFPRLVGPDFFGCAEPANTFL


    Database link: P24347

    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI

  • Positive control

    • WB: HEK-293T transfected with pCMV6-ENTRY MMP11; MDA-MB-231 and T-47D whole cell lysates.

Properties

Applications

Our Abpromise guarantee covers the use of ab236510 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500. Predicted molecular weight: 55 kDa.

Target

  • Function

    May play an important role in the progression of epithelial malignancies.

  • Tissue specificity

    Specifically expressed in stromal cells of breast carcinomas.

  • Sequence similarities

    Belongs to the peptidase M10A family.
    Contains 4 hemopexin-like domains.

  • Domain

    The conserved cysteine present in the cysteine-switch motif binds the catalytic zinc ion, thus inhibiting the enzyme. The dissociation of the cysteine from the zinc ion upon the activation-peptide release activates the enzyme.

  • Post-translational
    modifications

    The precursor is cleaved by a furin endopeptidase.

  • Cellular localization

    Secreted > extracellular space > extracellular matrix.
  • Information by UniProt

  • Database links

    • Alternative names

      • Matrix Metalloproteinase 11 antibody
      • Matrix metalloproteinase-11 antibody
      • MMP-11 antibody
      • Mmp11 antibody
      • MMP11_HUMAN antibody
      • SL 3 antibody
      • SL-3 antibody
      • SL3 antibody
      • ST3 antibody
      • STMY3 antibody
      • Stromelysin 3 antibody
      • Stromelysin III antibody
      • Stromelysin-3 antibody
      see all

    Images

    • Western blot - Anti-MMP11 antibody [OTI3E10] (ab236510)
      Western blot - Anti-MMP11 antibody [OTI3E10] (ab236510)
      All lanes : Anti-MMP11 antibody [OTI3E10] (ab236510) at 1/500 dilution

      Lane 1 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) transfected with pCMV6-ENTRY control
      Lane 2 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) transfected with pCMV6-ENTRY MMP11

      Lysates/proteins at 5 µg per lane.

      Predicted band size: 55 kDa

    • Western blot - Anti-MMP11 antibody [OTI3E10] (ab236510)
      Western blot - Anti-MMP11 antibody [OTI3E10] (ab236510)
      All lanes : Anti-MMP11 antibody [OTI3E10] (ab236510) at 1/500 dilution

      Lane 1 : MDA-MB-231 (human breast adenocarcinoma cell line) whole cell lysate
      Lane 2 : T-47D (human ductal breast epithelial tumor cell line) whole cell lysate

      Lysates/proteins at 35 µg per lane.

      Predicted band size: 55 kDa

    References

    ab236510 has not yet been referenced specifically in any publications.

    Publishing research using ab236510? Please let us know so that we can cite the reference in this datasheet.

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