Anti-MMP12 antibody (ab128030)
Key features and details
- Rabbit polyclonal to MMP12
- Suitable for: IHC-P
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-MMP12 antibody
See all MMP12 primary antibodies -
Description
Rabbit polyclonal to MMP12 -
Host species
Rabbit -
Tested applications
Suitable for: IHC-Pmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse
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Immunogen
Synthetic peptide surrounding amino acid 460 of Human MMP12.
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Positive control
- Human lung, macrophages
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. -
Storage buffer
Preservative: 0.02% Thimerosal (merthiolate)
Constituents: 69% PBS, 30% Glycerol -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab128030 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| IHC-P |
Use a concentration of 5 µg/ml.
|
| Notes |
|---|
|
IHC-P
Use a concentration of 5 µg/ml. |
Target
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Function
May be involved in tissue injury and remodeling. Has significant elastolytic activity. Can accept large and small amino acids at the P1' site, but has a preference for leucine. Aromatic or hydrophobic residues are preferred at the P1 site, with small hydrophobic residues (preferably alanine) occupying P3. -
Tissue specificity
Found in alveolar macrophages but not in peripheral blood monocytes. -
Sequence similarities
Belongs to the peptidase M10A family.
Contains 4 hemopexin-like domains. -
Domain
The conserved cysteine present in the cysteine-switch motif binds the catalytic zinc ion, thus inhibiting the enzyme. The dissociation of the cysteine from the zinc ion upon the activation-peptide release activates the enzyme. -
Cellular localization
Secreted > extracellular space > extracellular matrix. - Information by UniProt
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Database links
- Entrez Gene: 4321 Human
- Entrez Gene: 17381 Mouse
- Omim: 601046 Human
- SwissProt: P39900 Human
- SwissProt: P34960 Mouse
- Unigene: 1695 Human
- Unigene: 2055 Mouse
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Form
The presence of a 22kDa truncated active form of MMP-12 in vitro as well as in vivo due to the internal autolytic cleavage of the C-terminus. -
Alternative names
- EC 3.4.24.65 antibody
- HME antibody
- Macrophage elastase antibody
see all
Images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MMP12 antibody (ab128030)ab128030, at a concentration of 5 µg/ml, staining MMP12 in formalin fixed, paraffin embedded Human lung macrophages by Immunohistochemistry.
Datasheets and documents
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SDS download
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Datasheet download
References (1)
ab128030 has been referenced in 1 publication.
- Reed HO et al. Lymphatic impairment leads to pulmonary tertiary lymphoid organ formation and alveolar damage. J Clin Invest 129:2514-2526 (2019). PubMed: 30946031