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Cardiovascular Angiogenesis Adhesion / ECM Matrix Metalloproteinases MMP
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Anti-MMP12 antibody (ab128030)

  • Datasheet
  • SDS
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MMP12 antibody (ab128030)

    Key features and details

    • Rabbit polyclonal to MMP12
    • Suitable for: IHC-P
    • Reacts with: Human
    • Isotype: IgG

    You may also be interested in

    Protein
    Product image
    Recombinant Human MMP12 protein (ab131994)
    Secondary
    Product image
    Goat Anti-Rabbit IgG H&L (HRP) (ab205718)

    View more associated products

    Overview

    • Product name

      Anti-MMP12 antibody
      See all MMP12 primary antibodies
    • Description

      Rabbit polyclonal to MMP12
    • Host species

      Rabbit
    • Tested applications

      Suitable for: IHC-Pmore details
    • Species reactivity

      Reacts with: Human
      Predicted to work with: Mouse
    • Immunogen

      Synthetic peptide surrounding amino acid 460 of Human MMP12.

    • Positive control

      • Human lung, macrophages
    • General notes

      The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

      If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

    Properties

    • Form

      Liquid
    • Storage instructions

      Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term.
    • Storage buffer

      Preservative: 0.02% Thimerosal (merthiolate)
      Constituents: 69% PBS, 30% Glycerol
    • Concentration information loading...
    • Purity

      Protein A purified
    • Clonality

      Polyclonal
    • Isotype

      IgG
    • Research areas

      • Cardiovascular
      • Angiogenesis
      • Adhesion / ECM
      • Matrix Metalloproteinases
      • MMP
      • Signal Transduction
      • Cytoskeleton / ECM
      • Extracellular Matrix
      • ECM Enzymes
      • MMP
      • Cancer
      • Invasion/microenvironment
      • Angiogenesis
      • ECM enzymes
      • MMPs
      • Cancer
      • Tumor biomarkers
      • Enzymes
      • MMPs
      • Cardiovascular
      • Atherosclerosis
      • Thrombosis
      • Other

    Associated products

    • Compatible Secondaries

      • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
      • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
    • Isotype control

      • Rabbit IgG, polyclonal - Isotype Control (ChIP Grade) (ab171870)
    • Recombinant Protein

      • Recombinant Human MMP12 protein (ab131994)

    Applications

    The Abpromise guarantee

    Our Abpromise guarantee covers the use of ab128030 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Application Abreviews Notes
    IHC-P
    Use a concentration of 5 µg/ml.
    Notes
    IHC-P
    Use a concentration of 5 µg/ml.

    Target

    • Function

      May be involved in tissue injury and remodeling. Has significant elastolytic activity. Can accept large and small amino acids at the P1' site, but has a preference for leucine. Aromatic or hydrophobic residues are preferred at the P1 site, with small hydrophobic residues (preferably alanine) occupying P3.
    • Tissue specificity

      Found in alveolar macrophages but not in peripheral blood monocytes.
    • Sequence similarities

      Belongs to the peptidase M10A family.
      Contains 4 hemopexin-like domains.
    • Domain

      The conserved cysteine present in the cysteine-switch motif binds the catalytic zinc ion, thus inhibiting the enzyme. The dissociation of the cysteine from the zinc ion upon the activation-peptide release activates the enzyme.
    • Cellular localization

      Secreted > extracellular space > extracellular matrix.
    • Target information above from: UniProt accession P39900 The UniProt Consortium
      The Universal Protein Resource (UniProt) in 2010
      Nucleic Acids Res. 38:D142-D148 (2010) .

      Information by UniProt
    • Database links

      • Entrez Gene: 4321 Human
      • Entrez Gene: 17381 Mouse
      • Omim: 601046 Human
      • SwissProt: P39900 Human
      • SwissProt: P34960 Mouse
      • Unigene: 1695 Human
      • Unigene: 2055 Mouse
      • Form

        The presence of a 22kDa truncated active form of MMP-12 in vitro as well as in vivo due to the internal autolytic cleavage of the C-terminus.
      • Alternative names

        • EC 3.4.24.65 antibody
        • HME antibody
        • Macrophage elastase antibody
        • Macrophage metalloelastase antibody
        • Macrophage metaloelastase antibody
        • Matrix metallopeptidase 12 (macrophage elastase) antibody
        • Matrix metalloprotease 12 antibody
        • Matrix metalloproteinase-12 antibody
        • ME antibody
        • MGC138506 antibody
        • MME antibody
        • MMP 12 antibody
        • MMP-12 antibody
        • Mmp12 antibody
        • MMP12_HUMAN antibody
        see all

      Images

      • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MMP12 antibody (ab128030)
        Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MMP12 antibody (ab128030)
        ab128030, at a concentration of 5 µg/ml, staining MMP12 in formalin fixed, paraffin embedded Human lung macrophages by Immunohistochemistry.

      Protocols

      • Immunohistochemistry protocols

      Click here to view the general protocols

      Datasheets and documents

      • SDS download

      • Datasheet download

        Download

      References (1)

      Publishing research using ab128030? Please let us know so that we can cite the reference in this datasheet.

      ab128030 has been referenced in 1 publication.

      • Reed HO  et al. Lymphatic impairment leads to pulmonary tertiary lymphoid organ formation and alveolar damage. J Clin Invest 129:2514-2526 (2019). PubMed: 30946031

      Customer reviews and Q&As

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