Anti-MMP12 antibody (ab128030)

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Overview

  • Product name

    Anti-MMP12 antibody
    See all MMP12 primary antibodies

  • Description

    Rabbit polyclonal to MMP12

  • Host species

    Rabbit

  • Tested applications

    Suitable for: WB, IHC-Pmore details

  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse

  • Immunogen

    Synthetic peptide surrounding amino acid 460 of Human MMP12.

  • Positive control

    • Human lung, macrophages

Properties

Applications

Our Abpromise guarantee covers the use of ab128030 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use at an assay dependent concentration. Predicted molecular weight: 54 kDa.
IHC-P Use a concentration of 5 µg/ml.

Target

  • Function

    May be involved in tissue injury and remodeling. Has significant elastolytic activity. Can accept large and small amino acids at the P1' site, but has a preference for leucine. Aromatic or hydrophobic residues are preferred at the P1 site, with small hydrophobic residues (preferably alanine) occupying P3.

  • Tissue specificity

    Found in alveolar macrophages but not in peripheral blood monocytes.

  • Sequence similarities

    Belongs to the peptidase M10A family.
    Contains 4 hemopexin-like domains.

  • Domain

    The conserved cysteine present in the cysteine-switch motif binds the catalytic zinc ion, thus inhibiting the enzyme. The dissociation of the cysteine from the zinc ion upon the activation-peptide release activates the enzyme.

  • Cellular localization

    Secreted > extracellular space > extracellular matrix.
  • Information by UniProt

  • Database links

    • Form

      The presence of a 22kDa truncated active form of MMP-12 in vitro as well as in vivo due to the internal autolytic cleavage of the C-terminus.

    • Alternative names

      • EC 3.4.24.65 antibody
      • HME antibody
      • Macrophage elastase antibody
      • Macrophage metalloelastase antibody
      • Macrophage metaloelastase antibody
      • Matrix metallopeptidase 12 (macrophage elastase) antibody
      • Matrix metalloprotease 12 antibody
      • Matrix metalloproteinase-12 antibody
      • ME antibody
      • MGC138506 antibody
      • MME antibody
      • MMP 12 antibody
      • MMP-12 antibody
      • Mmp12 antibody
      • MMP12_HUMAN antibody
      see all

    Images

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MMP12 antibody (ab128030)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MMP12 antibody (ab128030)
      ab128030, at a concentration of 5 µg/ml, staining MMP12 in formalin fixed, paraffin embedded Human lung macrophages by Immunohistochemistry.

    References

    This product has been referenced in:

    • Reed HO  et al. Lymphatic impairment leads to pulmonary tertiary lymphoid organ formation and alveolar damage. J Clin Invest 129:2514-2526 (2019). Read more (PubMed: 30946031) »
    See 1 Publication for this product

    Publishing research using ab128030? Please let us know so that we can cite the reference in this datasheet.

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