Overview

  • Product name

  • Description

    Rabbit polyclonal to MMP12
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IHC-P, ICC/IFmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Synthetic peptide, corresponding to a region within internal sequence amino acids 213 - 307 of Human MMP12 (NP_002417).

  • Positive control

    • 293T, H1299 and HelaS3 whole cell lysates; DLD1 xenograft tissue; H1299 cells.

Properties

Applications

Our Abpromise guarantee covers the use of ab137443 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/3000. Predicted molecular weight: 54 kDa.
IHC-P 1/100 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
ICC/IF 1/100 - 1/1000.

Target

  • Function

    May be involved in tissue injury and remodeling. Has significant elastolytic activity. Can accept large and small amino acids at the P1' site, but has a preference for leucine. Aromatic or hydrophobic residues are preferred at the P1 site, with small hydrophobic residues (preferably alanine) occupying P3.
  • Tissue specificity

    Found in alveolar macrophages but not in peripheral blood monocytes.
  • Sequence similarities

    Belongs to the peptidase M10A family.
    Contains 4 hemopexin-like domains.
  • Domain

    The conserved cysteine present in the cysteine-switch motif binds the catalytic zinc ion, thus inhibiting the enzyme. The dissociation of the cysteine from the zinc ion upon the activation-peptide release activates the enzyme.
  • Cellular localization

    Secreted > extracellular space > extracellular matrix.
  • Information by UniProt
  • Database links

  • Form

    The presence of a 22kDa truncated active form of MMP-12 in vitro as well as in vivo due to the internal autolytic cleavage of the C-terminus.
  • Alternative names

    • EC 3.4.24.65 antibody
    • HME antibody
    • Macrophage elastase antibody
    • Macrophage metalloelastase antibody
    • Macrophage metaloelastase antibody
    • Matrix metallopeptidase 12 (macrophage elastase) antibody
    • Matrix metalloprotease 12 antibody
    • Matrix metalloproteinase-12 antibody
    • ME antibody
    • MGC138506 antibody
    • MME antibody
    • MMP 12 antibody
    • MMP-12 antibody
    • Mmp12 antibody
    • MMP12_HUMAN antibody
    see all

Images

  • Anti-MMP12 antibody (ab137443) at 1/1000 dilution + 293T whole cell lysate at 30 µg

    Predicted band size: 54 kDa



    10% SDS PAGE
  • Immunohistochemical analysis of parafin-embedded DLD1 xenograft tissue labelling MMP12 with ab137443 at 1/500 dilution.
  • Immunofluorescence analysis of methanol-fixed H1299 cells labelling MMP12 with ab137443 at 1/500 dilution. The image in the lower panel is costained with Hoechst 33342.

References

ab137443 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

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