Overview

  • Product name
    Anti-MMP12 antibody
    See all MMP12 primary antibodies
  • Description
    Chicken polyclonal to MMP12
  • Host species
    Chicken
  • Tested applications
    Suitable for: WBmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Fusion protein, corresponding to amino acids 1-33 of Human MMP12

Properties

Applications

Our Abpromise guarantee covers the use of ab26832 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/5000. Predicted molecular weight: 54 kDa.

Target

  • Function
    May be involved in tissue injury and remodeling. Has significant elastolytic activity. Can accept large and small amino acids at the P1' site, but has a preference for leucine. Aromatic or hydrophobic residues are preferred at the P1 site, with small hydrophobic residues (preferably alanine) occupying P3.
  • Tissue specificity
    Found in alveolar macrophages but not in peripheral blood monocytes.
  • Sequence similarities
    Belongs to the peptidase M10A family.
    Contains 4 hemopexin-like domains.
  • Domain
    The conserved cysteine present in the cysteine-switch motif binds the catalytic zinc ion, thus inhibiting the enzyme. The dissociation of the cysteine from the zinc ion upon the activation-peptide release activates the enzyme.
  • Cellular localization
    Secreted > extracellular space > extracellular matrix.
  • Information by UniProt
  • Database links
    • Form
      The presence of a 22kDa truncated active form of MMP-12 in vitro as well as in vivo due to the internal autolytic cleavage of the C-terminus.
    • Alternative names
      • EC 3.4.24.65 antibody
      • HME antibody
      • Macrophage elastase antibody
      • Macrophage metalloelastase antibody
      • Macrophage metaloelastase antibody
      • Matrix metallopeptidase 12 (macrophage elastase) antibody
      • Matrix metalloprotease 12 antibody
      • Matrix metalloproteinase-12 antibody
      • ME antibody
      • MGC138506 antibody
      • MME antibody
      • MMP 12 antibody
      • MMP-12 antibody
      • Mmp12 antibody
      • MMP12_HUMAN antibody
      see all

    Images

    • Western blot - Anti-MMP12 antibody (ab26832)
      Western blot - Anti-MMP12 antibody (ab26832)
      Anti-MMP12 antibody (ab26832) at 1/5000 dilution + human MMP12 protein 400ng (1-33amino acids)

      Secondary
      goat-anti-IgY/Fc-HRP

      Developed using the ECL technique.

      Predicted band size: 54 kDa
      Observed band size: 15 kDa (why is the actual band size different from the predicted?)



      Different expression length of fusion protein will result in different MW of MMP 12.

    References

    This product has been referenced in:
    • Alarab M  et al. Expression of Extracellular Matrix-Remodeling Proteins Is Altered in Vaginal Tissue of Premenopausal Women With Severe Pelvic Organ Prolapse. Reprod Sci N/A:N/A (2013). WB, IHC-Fr ; Human . Read more (PubMed: 24343133) »

    See 1 Publication for this product

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