Anti-MMP12 antibody (ab26832)
- Datasheet
- References (1)
- Protocols
Overview
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Product nameAnti-MMP12 antibody
See all MMP12 primary antibodies -
DescriptionChicken polyclonal to MMP12
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Host speciesChicken
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Tested applicationsSuitable for: WBmore details
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Species reactivityReacts with: Human
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Immunogen
Fusion protein, corresponding to amino acids 1-33 of Human MMP12
Properties
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FormLiquid
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Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term.
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Storage bufferConstituent: PBS
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Concentration information loading... -
PurityImmunogen affinity purified
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ClonalityPolyclonal
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IsotypeIgY
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Research areas
Associated products
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Isotype control
Applications
Our Abpromise guarantee covers the use of ab26832 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | 1/5000. Predicted molecular weight: 54 kDa. |
Target
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FunctionMay be involved in tissue injury and remodeling. Has significant elastolytic activity. Can accept large and small amino acids at the P1' site, but has a preference for leucine. Aromatic or hydrophobic residues are preferred at the P1 site, with small hydrophobic residues (preferably alanine) occupying P3.
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Tissue specificityFound in alveolar macrophages but not in peripheral blood monocytes.
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Sequence similaritiesBelongs to the peptidase M10A family.
Contains 4 hemopexin-like domains. -
DomainThe conserved cysteine present in the cysteine-switch motif binds the catalytic zinc ion, thus inhibiting the enzyme. The dissociation of the cysteine from the zinc ion upon the activation-peptide release activates the enzyme.
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Cellular localizationSecreted > extracellular space > extracellular matrix.
- Information by UniProt
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Database links
- Entrez Gene: 4321 Human
- Omim: 601046 Human
- SwissProt: P39900 Human
- Unigene: 1695 Human
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FormThe presence of a 22kDa truncated active form of MMP-12 in vitro as well as in vivo due to the internal autolytic cleavage of the C-terminus.
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Alternative names
- EC 3.4.24.65 antibody
- HME antibody
- Macrophage elastase antibody
see all
Images
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Western blot - Anti-MMP12 antibody (ab26832)Anti-MMP12 antibody (ab26832) at 1/5000 dilution + human MMP12 protein 400ng (1-33amino acids)
Secondary
goat-anti-IgY/Fc-HRP
Developed using the ECL technique.
Predicted band size: 54 kDa
Observed band size: 15 kDa why is the actual band size different from the predicted?
Different expression length of fusion protein will result in different MW of MMP 12.
Protocols
Datasheets and documents
References
This product has been referenced in:
- Alarab M et al. Expression of Extracellular Matrix-Remodeling Proteins Is Altered in Vaginal Tissue of Premenopausal Women With Severe Pelvic Organ Prolapse. Reprod Sci N/A:N/A (2013). WB, IHC-Fr ; Human . Read more (PubMed: 24343133) »
