Recombinant Anti-MMP12 antibody [EP1261Y] (ab52897)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EP1261Y] to MMP12
- Suitable for: WB, IP
- Reacts with: Human, Recombinant fragment
Related conjugates and formulations
Overview
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Product name
Anti-MMP12 antibody [EP1261Y]
See all MMP12 primary antibodies -
Description
Rabbit monoclonal [EP1261Y] to MMP12 -
Host species
Rabbit -
Tested applications
Suitable for: WB, IPmore details
Unsuitable for: Flow Cyt or IHC-P -
Species reactivity
Reacts with: Human, Recombinant fragment -
Immunogen
Synthetic peptide within Human MMP12 aa 450 to the C-terminus (C terminal). The exact sequence is proprietary.
Database link: P39900 -
General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Stable for 12 months at -20°C. -
Storage buffer
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol, 0.05% BSA -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EP1261Y -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Assay kits
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab52897 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
1/1000 - 1/10000. Detects a band of approximately 54 kDa (predicted molecular weight: 54 kDa).
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IP |
1/20.
For unpurified use at 1/30. |
Notes |
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WB
1/1000 - 1/10000. Detects a band of approximately 54 kDa (predicted molecular weight: 54 kDa). |
IP
1/20. For unpurified use at 1/30. |
Target
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Function
May be involved in tissue injury and remodeling. Has significant elastolytic activity. Can accept large and small amino acids at the P1' site, but has a preference for leucine. Aromatic or hydrophobic residues are preferred at the P1 site, with small hydrophobic residues (preferably alanine) occupying P3. -
Tissue specificity
Found in alveolar macrophages but not in peripheral blood monocytes. -
Sequence similarities
Belongs to the peptidase M10A family.
Contains 4 hemopexin-like domains. -
Domain
The conserved cysteine present in the cysteine-switch motif binds the catalytic zinc ion, thus inhibiting the enzyme. The dissociation of the cysteine from the zinc ion upon the activation-peptide release activates the enzyme. -
Cellular localization
Secreted > extracellular space > extracellular matrix. - Information by UniProt
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Database links
- Entrez Gene: 4321 Human
- Omim: 601046 Human
- SwissProt: P39900 Human
- Unigene: 1695 Human
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Form
The presence of a 22kDa truncated active form of MMP-12 in vitro as well as in vivo due to the internal autolytic cleavage of the C-terminus. -
Alternative names
- EC 3.4.24.65 antibody
- HME antibody
- Macrophage elastase antibody
see all
Images
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All lanes : Anti-MMP12 antibody [EP1261Y] (ab52897) at 1/2000 dilution (purified)
Lane 1 : HT-1080 cell lysate
Lane 2 : WI-38 cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/1000 dilution (Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated)
Predicted band size: 54 kDaBlocking buffer and concentration: 5% NFDM/TBST.
Diluting buffer and concentration: 5% NFDM /TBST. -
ab52897 (purified) at 1/20 immunoprecipitating Glutamate Receptor 1 in HeLa whole cell lysate. 10 ug of cell lysate was present in the input. For western blotting, a HRP-conjugated Veriblot for IP Detection Reagent (ab131366) (1/1,500) was used for detection. A rabbit monoclonal IgG (ab172730) was used intead of ab128913 as a negative control (Lane 3).
Blocking buffer and concentration: 5% NFDM/TBST.
Diluting buffer and concentration: 5% NFDM /TBST.
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Based on Western blot, unpurified ab52897 recognizes both pro and active MMP. The propeptide is 54 kDa, and 45 kDa and 22 kDa are active forms. The primary was used at 1:100 to detect recombinant MMP12 protein, but can be diluted more to decrease the intensity of the bands.
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
References (34)
ab52897 has been referenced in 34 publications.
- Wu J et al. NPTX1 inhibits pancreatic cancer cell proliferation and migration and enhances chemotherapy sensitivity by targeting RBM10. Oncol Lett 23:154 (2022). PubMed: 35836482
- Li J et al. Interference of PTK6/GAB1 signaling inhibits cell proliferation, invasion, and migration of cervical cancer cells. Mol Med Rep 26:N/A (2022). PubMed: 35894144
- Makise M et al. Overexpression of the nucleoporin Nup88 stimulates migration and invasion of HeLa cells. Histochem Cell Biol 156:409-421 (2021). PubMed: 34331103
- Namba M et al. Creation of X-linked Alport syndrome rat model with Col4a5 deficiency. Sci Rep 11:20836 (2021). PubMed: 34675305
- Huang HQ et al. Autophagy Promotes Cigarette Smoke-Initiated and Elastin-Driven Bronchitis-Like Airway Inflammation in Mice. Front Immunol 12:594330 (2021). PubMed: 33828547