Recombinant
RabMAb

Recombinant Anti-MMP12 antibody [EP1261Y] - BSA and Azide free (ab239851)

Overview

  • Product name

    Anti-MMP12 antibody [EP1261Y] - BSA and Azide free
    See all MMP12 primary antibodies
  • Description

    Rabbit monoclonal [EP1261Y] to MMP12 - BSA and Azide free
  • Host species

    Rabbit
  • Tested applications

    Suitable for: IP, WBmore details
    Unsuitable for: Flow Cyt or IHC-P
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Synthetic peptide within Human MMP12 aa 450 to the C-terminus (C terminal). The exact sequence is proprietary.
    Database link: P39900

  • General notes

    Ab239851 is the carrier-free version of ab52897. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

    ab239851 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab239851 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IP Use at an assay dependent concentration.
WB Use at an assay dependent concentration. Detects a band of approximately 54 kDa (predicted molecular weight: 54 kDa).
  • Application notes
    Is unsuitable for Flow Cyt or IHC-P.
  • Target

    • Function

      May be involved in tissue injury and remodeling. Has significant elastolytic activity. Can accept large and small amino acids at the P1' site, but has a preference for leucine. Aromatic or hydrophobic residues are preferred at the P1 site, with small hydrophobic residues (preferably alanine) occupying P3.
    • Tissue specificity

      Found in alveolar macrophages but not in peripheral blood monocytes.
    • Sequence similarities

      Belongs to the peptidase M10A family.
      Contains 4 hemopexin-like domains.
    • Domain

      The conserved cysteine present in the cysteine-switch motif binds the catalytic zinc ion, thus inhibiting the enzyme. The dissociation of the cysteine from the zinc ion upon the activation-peptide release activates the enzyme.
    • Cellular localization

      Secreted > extracellular space > extracellular matrix.
    • Information by UniProt
    • Database links

    • Form

      The presence of a 22kDa truncated active form of MMP-12 in vitro as well as in vivo due to the internal autolytic cleavage of the C-terminus.
    • Alternative names

      • EC 3.4.24.65 antibody
      • HME antibody
      • Macrophage elastase antibody
      • Macrophage metalloelastase antibody
      • Macrophage metaloelastase antibody
      • Matrix metallopeptidase 12 (macrophage elastase) antibody
      • Matrix metalloprotease 12 antibody
      • Matrix metalloproteinase-12 antibody
      • ME antibody
      • MGC138506 antibody
      • MME antibody
      • MMP 12 antibody
      • MMP-12 antibody
      • Mmp12 antibody
      • MMP12_HUMAN antibody
      see all

    Images

    • ab52897 (purified) at 1/20 immunoprecipitating Glutamate Receptor 1 in HeLa whole cell lysate. 10 ug of cell lysate was present in the input. For western blotting, a HRP-conjugated Veriblot for IP Detection Reagent (ab131366) was used for detection at 1/1,500  dilution. A rabbit monoclonal IgG (ab172730) was used intead of ab128913 as a negative control (Lane 3).

      Blocking buffer and concentration: 5% NFDM/TBST.

      Diluting buffer and concentration: 5% NFDM /TBST.

      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab52897).

    References

    ab239851 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

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    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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